Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,786	G→A	T165I (ACT→ATT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,786	0	G	A	100.0%	70.9 / NA	25	T165I (ACT→ATT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

AGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAAT  >  NC_000913/3120554‑3121030                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     agagGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACAAGCCCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGacac                                                                                                                                                                                                                                     >  1:265094/1‑250 (MQ=255)                    aCAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGACCGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCACACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCCTATAACgg                                                                                                                                                                                                                 >  2:225086/1‑251 (MQ=255)                         gAAGCTTCCACCAGCACCAAGCAACGCTTGCCACGTCTCTTTCACCCCTTTCCACCCGTCAACCATTACTACCACCCAAACCGGCACAAGAACCGACAGGAAAGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTGACCTGCCCGGCGACCAGAATCGGCACGCCCAACGCCCCAAACGCAACCCGCGCAATATTGGCCATCAGACACACCCCCACCGCGTCTAACgggtac                                                                                                                                                                                                             >  2:37142/1‑246 (MQ=255)                                cccccAGCAACCAGCGCCGCTGGCCCCGTCTCTTTCCCCCCTTTCCAGCCGTCCATCATGGCTCCCAGCCAGAACGGCACAAGACCCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAg                                                                                                                                                                                                     <  2:43740/251‑1 (MQ=255)                                       cAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGac                                                                                                                                                                                                                                       <  2:98740/210‑1 (MQ=255)                                       cAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGac                                                                                                                                                                                                                                       >  1:98740/1‑210 (MQ=255)                                       cAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCccacc                                                                                                                                                                                              >  1:111576/1‑251 (MQ=255)                                           cAGCGCCGCTGAGCACGACTCATTCACCACTTCCCAGACGTCCATCCTTGCTACCAGCCAGAACGGCACAACAACCGACAGGAACCGTCACTGACGTCCCGCCATTGCACCAATGTGGAACGGATCGATTCCCGTTACCTGACCAGCGACCAGAATCGGCACGACCAACGCACCAACCGCCACCGGCGCCATATTGGCAATCAGACACAGCCCCGCCGCGCATAACGGTTTCACGCCCAGGCCCACcagc                                                                                                                                                                                           >  2:306535/1‑250 (MQ=255)                                              cgccgcTGGCCACGTCTCTTCCACCCCTTTCCAGCCGTCCATCATGGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAgcg                                                                                                                                                                                       <  2:97152/251‑1 (MQ=255)                                                 cgcTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGACAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCAc                                                                                                                                                                                    >  1:220557/1‑251 (MQ=255)                                                     ggCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCCGCAGCGCACCggg                                                                                                                                                                                >  2:199685/1‑250 (MQ=255)                                                              tctTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCAc                                                                                                                                                                       >  2:8295/1‑251 (MQ=255)                                                                 ttCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCgg                                                                                                                                                                    >  1:135679/1‑251 (MQ=255)                                                                                                                  gAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCa                                                                                                                   <  1:104175/251‑1 (MQ=255)                                                                                                                             aaCGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAacac                                                                                                        >  1:41252/1‑251 (MQ=255)                                                                                                                                     cTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAAc                                                                                                >  2:35984/1‑251 (MQ=255)                                                                                                                                               cccATTGCGCCAATGTGGAACGGATCGATTCCCGTTCCCGGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcgtc                                                                                      <  2:41252/250‑1 (MQ=255)                                                                                                                                                     gcgcCAATGTGGAACGGATCGATTCCCGTAACCTGACCGGCAACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGa                                                                                 <  1:145798/250‑1 (MQ=255)                                                                                                                                                                                             cGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTg                                        <  1:44387/251‑1 (MQ=255)                                                                                                                                                                                                ccAGAATAGGCCCGCCCAACGCACCAAACGCCACCGGCGCAATATTGGCAATCAGGCACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCa                                     <  1:227114/251‑1 (MQ=255)                                                                                                                                                                                                ccAGAACCGGCACGCCCACCGCACCAAACGCCAACGGCGCAATATTGGCAAGCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCCCCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCa                                     <  2:91470/251‑1 (MQ=255)                                                                                                                                                                                                       cgccacccccAACGCCCCAAACCCCCCCGGCGCAATATTGGCAACCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTa                              <  2:111576/244‑1 (MQ=255)                                                                                                                                                                                                                 aaCGCACCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCAGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCAAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGGGTTGGAGATAACCGAGCGGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATAc                      >  2:183117/1‑249 (MQ=255)                                                                                                                                                                                                                      aCCAAACGCCACCGGCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAaca               <  1:199685/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCGCAATATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACAACGCCGCGACaat  >  1:244544/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     AGAGGTAAAGAACTGAGTGACAGCGAAGCTTCCCCCAGCAACCAGCGCCGCTGGCCACGTCTCTTTCACCCCTTTCCAGCCGTCCATCATTGCTACCAGCCAGAACGGCACAAGAACCGACAGGAACGGTAACTGACGTCCCGCCATTGCGCCAATGTGGAACGGATCGATTCCCGTTACCTGACCGGCGACCAGAATCGGCACGCCCAACGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAAT  >  NC_000913/3120554‑3121030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑