Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,887	G→A	S152N (AGC→AAC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,887	0	G	A	100.0%	105.9 / NA	31	S152N (AGC→AAC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/17);  total (14/17)

AACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGA  >  NC_000913/3104667‑3105128                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  aaCTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGACAAACCg                                                                                                                                                                                                                     >  2:173891/1‑251 (MQ=255)                aCGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCgc                                                                                                                                                                                                        >  1:2464/1‑249 (MQ=255)                     tACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACg                                                                                                                                                                                                 >  1:161494/1‑251 (MQ=255)                      aCTATCGAACCCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACg                                                                                                                                                                                                 <  2:161494/250‑1 (MQ=255)                       cTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACggg                                                                                                                                                                                               >  1:249819/1‑251 (MQ=255)                                              gAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCAc                                                                                                                                                                        <  2:58955/251‑1 (MQ=255)                                                aCCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCAcc                                                                                                                                                                       >  2:9908/1‑250 (MQ=255)                                                      cgcgCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACa                                                                                                                                                                <  2:112988/251‑1 (MQ=255)                                                         gcATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATgg                                                                                                                                                             >  1:203200/1‑251 (MQ=255)                                                         gcATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCATTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATgg                                                                                                                                                             <  1:5327/251‑1 (MQ=255)                                                                       cAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCtt                                                                                                                                               <  1:9908/251‑1 (MQ=255)                                                                        aaTTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTg                                                                                                                                              >  1:107716/1‑251 (MQ=255)                                                                        aaTTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGGTGATGATATTACGATTTCGAAAGAACCTTTCCGTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTGCAGCGTCTCCATTAACATGGTTCCGAACCGCCTTg                                                                                                                                              <  2:204736/251‑1 (MQ=255)                                                                              cAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATGTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAAc                                                                                                                                        <  1:166087/251‑1 (MQ=255)                                                                               aaaaCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACg                                                                                                                                       <  1:195413/251‑1 (MQ=255)                                                                                          gATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGaa                                                                                                                                                                                                                           <  1:258584/156‑1 (MQ=255)                                                                                          gATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGaa                                                                                                                                                                                                                           >  2:258584/1‑156 (MQ=255)                                                                                           aTGGGTGACGCTCCGAGTTCGGCCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGCCAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGCTTATCTGCTGAACAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACCAat                                                                                                                            >  2:274504/1‑250 (MQ=255)                                                                                               gTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGAGGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATAtctc                                                                                                                       <  2:98289/251‑1 (MQ=255)                                                                                                       ccGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGt                                                                                                               <  1:78784/251‑1 (MQ=255)                                                                                                                  cGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGCAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGCGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGt                                                                                                    >  2:294515/1‑251 (MQ=255)                                                                                                                          aTTCCTACGTTGATGATATTACGATTTCGACAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACCCCGGTCAGCCGATTCGCTGGTAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCCTATCATCTACAGCGTCACCATTAACCTGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCTTCACGGaa                                                                                             <  2:235959/250‑1 (MQ=255)                                                                                                                                               cGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTg                                                                       <  1:199688/251‑1 (MQ=255)                                                                                                                                                    tCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTc                                                                   <  1:104062/250‑1 (MQ=255)                                                                                                                                                     cGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTg                                                                 <  1:92756/251‑1 (MQ=255)                                                                                                                                                               tttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTg                                                                                                                                              <  1:52353/164‑1 (MQ=255)                                                                                                                                                               tttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTg                                                                                                                                              >  2:52353/1‑164 (MQ=255)                                                                                                                                                                               gaggtggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCtt                                       >  2:59719/3‑251 (MQ=255)                                                                                                                                                                                                             tGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATgc         >  1:111117/1‑251 (MQ=255)                                                                                                                                                                                                                gAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCt      >  1:194833/1‑251 (MQ=255)                                                                                                                                                                                                                    gTCGCGCAAACAACGTCGCGGACAATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCGCGGCATTGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGa  <  2:264630/251‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  AACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGA  >  NC_000913/3104667‑3105128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑