Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,260	T→C	V326A (GTG→GCG)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,260	0	T	C	100.0%	120.0 / NA	35	V326A (GTG→GCG)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/22);  total (13/22)

GCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACC  >  NC_000913/3089020‑3089504                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     gCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGGGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGgctgct                                                                                                                                                                                                                                            >  1:239231/1‑251 (MQ=255)          gccgcGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGc                                                                                                                                                                                                                                   <  1:62373/251‑1 (MQ=255)                              gTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGcc                                                                                                                                                                                                               >  1:206075/1‑250 (MQ=255)                                      gCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGt                                                                                                                                                                                                        <  1:305488/250‑1 (MQ=255)                                        agttAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAACATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGAACGTGGTTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTTAACCCACGCTCACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTAATCGGTACAATGGTGCCTATCGGTAtt                                                                                                                                                                                                     >  2:271602/4‑251 (MQ=255)                                        aGGTAATGAATCAATTCACCGTGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTTCCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTAtt                                                                                                                                                                                                     <  2:167969/251‑1 (MQ=255)                                                  gcaATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGt                                                                                                                                                                                           >  1:68965/1‑251 (MQ=255)                                                           cGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAgg                                                                                                                                                                                  >  2:124496/1‑250 (MQ=255)                                                                               aTTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGCCGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCAtgctg                                                                                                                                                              <  2:17273/251‑1 (MQ=255)                                                                                    gcgcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTAGCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGGATTTCGCCATCGCCATGCTGCTGAt                                                                                                                                                         <  2:285142/251‑1 (MQ=255)                                                                                      gcCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGt                                                                                                                                                       <  2:102945/251‑1 (MQ=255)                                                                                          aaaaTCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAAGGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTGTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTAt                                                                                                                                                   <  1:183311/251‑1 (MQ=255)                                                                                                             gTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGt                                                                                                                                <  2:195158/251‑1 (MQ=255)                                                                                                                                         ggggACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCt                                                                                                    <  1:144406/251‑1 (MQ=255)                                                                                                                                         ggggACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCt                                                                                                    >  1:101827/1‑251 (MQ=255)                                                                                                                                                  gATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAAtt                                                                                            <  2:273981/250‑1 (MQ=255)                                                                                                                                                                    cGTACTTGCCACCTTTATCGAAATAGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCcgcg                                                                         >  2:58655/1‑251 (MQ=255)                                                                                                                                                                       aCTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGAt                                                                       <  1:124496/250‑1 (MQ=255)                                                                                                                                                                            ccACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCGGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGc                                                                 <  2:20633/251‑1 (MQ=255)                                                                                                                                                                              cccTTGATCGCTATCGGCCTTGTTGACCGGTGGGGACGTAAACCAACGCTAACGCTGGGCTTCGTGGCGATGGCTGCTGGTATGGGCGTACTCGGTACAATTATGCACATCGGTATTCACTCTCCGTCGGCGCAGTATGTCGCCATCGCCATGCTGCTGATGTTTATGGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACGGTGCTCAGAAATTCATCCTCTGAAAGGCCGCGATTTTGGCAt                                                               <  2:271244/250‑1 (MQ=255)                                                                                                                                                                                  ttATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCCTCAcc                                                           >  2:195758/1‑251 (MQ=255)                                                                                                                                                                                  ttATCGCAATCGGCCTTGGTGCCCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTCTTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAc                                                            <  2:225983/250‑1 (MQ=255)                                                                                                                                                                                      cGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCt                                                       >  1:143179/1‑251 (MQ=255)                                                                                                                                                                                       gCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTc                                                      >  1:128408/1‑251 (MQ=255)                                                                                                                                                                                         aaTCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCa                                                    <  1:86046/251‑1 (MQ=255)                                                                                                                                                                                                     tgACCGCTGGGGACGTAAAACAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGAGTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCATCTGCGCCACTGCCACCAAc                                         <  2:3097/250‑1 (MQ=255)                                                                                                                                                                                                                 aCGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACa                            <  1:85724/251‑1 (MQ=255)                                                                                                                                                                                                                  cGTAAACCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACAt                           <  2:38904/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCCAACGCTAACGCTGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATc                       >  2:8704/1‑251 (MQ=255)                                                                                                                                                                                                                      aaCCAACGCTAACGCGTGGCTTCTTGTCGAGGGCGGCTGGAATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTTGGCGCCGTATTTCGGCATCGCCATGCTGCTGATGTTGATTGTCGGTTTTGCCATGAGTGCCGGTCCGCGGATTTGGGTACTGTGCTCCGAAAGTCAGCCGCTGAAAGGCCGCGATTTTGGCATCCCCTGCTCCACTGCCGCCAACTTTTTTGCCAAgatgatt                       <  2:26087/251‑2 (MQ=255)                                                                                                                                                                                                                              ctaacgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGCTTTTGGCATCCCCTGCTCCCCTGCCACCAACTGGATTGCCAACATGATCGTTGgcgc               >  2:261826/1‑251 (MQ=255)                                                                                                                                                                                                                                  cgctGGGCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACg           <  2:53762/251‑1 (MQ=255)                                                                                                                                                                                                                                      gggCTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTcc       <  1:6793/251‑1 (MQ=255)                                                                                                                                                                                                                                         cTTCCTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg     >  2:31247/1‑250 (MQ=255)                                                                                                                                                                                                                                            ccTGGCGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGAcc  <  1:232321/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAACGTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTAACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACC  >  NC_000913/3089020‑3089504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑