Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,449	G→A	L216L (TTG→TTA)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,449	0	G	A	96.9%	100.3 / ‑5.4	32	L216L (TTG→TTA)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/13);  minor base C (0/1);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTT  >  NC_000913/3065207‑3065685                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             ggTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATTGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTCCCCGACg                                                                                                                                                                                                                                       <  2:8770/250‑1 (MQ=255)       gTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGACGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCCGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTCCCCGCTATGTGCAAATCGATCCGCAAAAGATTGTCGCCGTCGTGGAAACCAACCTACCCGACGCCGGTa                                                                                                                                                                                                                                 >  2:263229/1‑250 (MQ=255)                            ggTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGCTTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCCAATCGATCCGAAAAAGATTGTCGCCGTCGTTGAAACCCACTTACCCGACGCCGGTCATATGCTGGATAAGCTAAATcc                                                                                                                                                                                                           >  2:155005/1‑251 (MQ=255)                                                        ggaagaaAGTGATCATTGAACCCAATCACCAACCCGAACCGCGCGTTGCAGAACTTGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGGGTGGCGATCTTCCATGGAATGGATCGGGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTg                                                                                                                                                                               <  2:24301/250‑1 (MQ=255)                                                               aGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGt                                                                                                                                                                        >  2:103615/1‑251 (MQ=255)                                                                tagataaTTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGCTTCCTGGCGCGCCACCGCGGCGCAATACCGTGTCGATCTTCCATGCAATGGATCGCGTGGGTACCCGCTATGTGCAAATCGATCCGAACAAGATTGTCGCCGTCGTGGCAACCCACTTACCCGTCGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCCGCAGATTTCCCAAAACGTGGTCACGtt                                                                                                                                                                       >  2:245412/7‑251 (MQ=255)                                                                 tGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCCAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTc                                                                                                                                                                      >  2:284274/1‑251 (MQ=255)                                                                  gATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTc                                                                                                                                                                      <  2:277075/250‑1 (MQ=255)                                                                    tCATTGAACTCAATCACTATCACGATCCGCGAGTCGAAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGGGTAGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGACGTCGGGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGACGATAACGTGGTCCAGTTCtt                                                                                                                                                                    >  2:115335/1‑250 (MQ=255)                                                                     cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTa                                                                                                                                                                   <  2:43208/250‑1 (MQ=255)                                                                        tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGc                                                                                                                                                               >  1:301784/1‑251 (MQ=255)                                                                        tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTACTGc                                                                                                                                                               >  2:140684/1‑251 (MQ=255)                                                                              cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGGGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaaa                                                                                                                                                         <  1:174662/251‑1 (MQ=255)                                                                                  cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgc                                                                                                                                                     >  2:3408/1‑251 (MQ=255)                                                                                      aTCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATCACGTGGTCACGTTCTTATTGCAGGAAATGgcgcc                                                                                                                                                  >  2:145774/1‑249 (MQ=255)                                                                                      aTCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCa                                                                                                                                                  <  1:127197/250‑1 (MQ=255)                                                                                          cGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGGCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCATATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGc                                                                                                                                             <  1:256060/251‑1 (MQ=255)                                                                                                                         gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCaa                                                                                                                >  1:161383/1‑249 (MQ=255)                                                                                                                                        ccACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAatat                                                                                               <  1:61780/251‑1 (MQ=255)                                                                                                                                         cACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATc                                                                                              <  1:206677/251‑1 (MQ=255)                                                                                                                                                    aaTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGt                                                                                   >  1:229287/1‑251 (MQ=255)                                                                                                                                                                        tGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAAttt                                                                                                                            <  2:169811/190‑1 (MQ=255)                                                                                                                                                                        tGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAAttt                                                                                                                            >  1:169811/1‑190 (MQ=255)                                                                                                                                                                                     gTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGGAAACCCGGTAATtcc                                                  >  2:95539/1‑251 (MQ=255)                                                                                                                                                                                                   tgtgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcag                                                                                                                                                                                                 <  2:228969/94‑1 (MQ=255)                                                                                                                                                                                                   tgtgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcag                                                                                                                                                                                                 >  1:228969/1‑94 (MQ=255)                                                                                                                                                                                                        aaaTCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCg                               <  1:152497/251‑1 (MQ=255)                                                                                                                                                                                                          aTCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGGAAACCCGGTAATTCCTCCGTTTATGATGTATTCgg                              >  2:116150/1‑250 (MQ=255)                                                                                                                                                                                                                      aaGATTGTCGCCGTCGTGGAAACCAACTTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGCTTATGATGTATTCGGAAGTGCTACAgg                  >  2:99805/1‑250 (MQ=255)                                                                                                                                                                                                                         aTTGTCGCCGTCGTGGAAACCAACGTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATc              <  2:148479/251‑1 (MQ=255)                                                                                                                                                                                                                                    agtGGAAACCGACTTACCCGACGCCGGTAATATGATGGAGAAGCAGAATCCCATGTGCCAGCAGAGTGCCGATAACGTGGTCACGTTCTTATGGCAGGAAATGGCGCGTGGGCGTATTCCTCCGGAATTTCGGCCGCTGCAAAGTGGCGGGGGAAATATCAATAATGCGGTAAGGGCGCTGCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGACGTATTGGGAAGTGCTATAGGTATTggtgatgaatt   <  2:197163/250‑8 (MQ=255)                                                                                                                                                                                                                                     gtGGAACCCACCTTACCAGAGGCCGGTAATCTCCTGGATAAGCAAAATCCCATGTGCCAGAAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCACCGTGGCGTGGGCAATATCACTTATGCGGAAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCCTTTATGCTGTCTTCGGACGTGCTACAGGAATAGGTGGTGCCttt  >  2:135672/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             GGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTT  >  NC_000913/3065207‑3065685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑