Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,566	G→A	W268* (TGG→TAG)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,566	0	G	A	100.0%	95.5 / NA	30	W268* (TGG→TAG)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/21);  total (9/21)

CGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCT  >  NC_000913/3009322‑3009802                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             cGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCg                                                                                                                                                                                                                                         <  1:270807/250‑1 (MQ=255)            tCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCAAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGGATTCCTCGGCAACGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGaa                                                                                                                                                                                                                              <  2:212806/250‑1 (MQ=255)                               gtTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCACCGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCGGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcg                                                                                                                                                                                                          <  2:257152/251‑1 (MQ=255)                                            gTTCTGCACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCctgcctgc                                                                                                                                                                                              >  1:148357/1‑250 (MQ=255)                                                  cACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAg                                                                                                                                                                                       <  1:56653/251‑1 (MQ=255)                                                       gAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGc                                                                                                                                                                                  <  2:6162/251‑1 (MQ=255)                                                          ggcgGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTTGGTTATGACGAATTCCTCGGCAAAGGCACCGTCACCTTTTCTGAAAGCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACATAAAGCTaa                                                                                                                                                                               <  2:305482/250‑1 (MQ=255)                                                                 aaaacgccattctgacgaTGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCACCGTCTGGGTTATGACGAATTCCTCGGCAAAGGCCCCCTCACCGTTTCTGAAATCTTCTTAACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCACCAGGCATGCAGTACAGAAAGCTCACGCGgtt                                                                                                                                                                        >  2:283343/18‑251 (MQ=255)                                                                 aTAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgtt                                                                                                                                                                        <  1:220952/251‑1 (MQ=255)                                                                   aaCGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttgt                                                                                                                                                                      <  1:288872/251‑1 (MQ=255)                                                                   aaCGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                                                                                                                                                                       <  1:6345/250‑1 (MQ=255)                                                                                aaaTGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAAc                                                                                                                                                         >  1:52168/1‑251 (MQ=255)                                                                                aaaTGGGGCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGGCTGGGTTAGGACGAAGTCCTCGGCAAAGGCACCCTAACCGGTTCTGAAATCTTCTTCGCGTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCTGTTGTTGATATGTACAAc                                                                                                                                                         <  2:273732/251‑1 (MQ=255)                                                                                    gggTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg                                                                                                                                                     >  2:178899/1‑251 (MQ=255)                                                                                      gTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGAc                                                                                                                                                   <  1:178899/251‑1 (MQ=255)                                                                                                                     ccAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCACGCCGTTGAGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGACGTCTCACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGAGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCa                                                                                                                     >  1:299889/1‑250 (MQ=255)                                                                                                                                   aTGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTGTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTc                                                                                                      <  1:32398/251‑1 (MQ=255)                                                                                                                                       cGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGa                                                                                                  <  2:252847/251‑1 (MQ=255)                                                                                                                                           ttCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                                                                                                  <  1:239882/231‑1 (MQ=255)                                                                                                                                           ttCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAAcc                                                                                                                  >  2:239882/1‑231 (MQ=255)                                                                                                                                                                         gAAAGCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCAcctt                                                                   <  2:221873/248‑3 (MQ=255)                                                                                                                                                                                     acaTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCAGTGGTGaa                                                    <  2:82917/251‑1 (MQ=255)                                                                                                                                                                                         ccccAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACTCGGTTGTTTCTATGTACTACTGCGACCGTCCGTCCTGGACTGGCCTGGTTGACCCAATCGAATGCGAGTTCCCATCCTGGAAAGTCGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGc                                                 <  1:133134/250‑1 (MQ=255)                                                                                                                                                                                         ccccAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGcc                                                >  1:165973/1‑251 (MQ=255)                                                                                                                                                                                                         tGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTg                                >  1:121101/1‑251 (MQ=255)                                                                                                                                                                                                          gTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTgg                               <  2:28130/251‑1 (MQ=255)                                                                                                                                                                                                                  cAGCTGCGCCGTTTCAATCGACCGTCGTCTGACCTAGGGCGAAGCCTGGGAAGACGAGCTGGATGAAATCCGCGTCCTGCCTGCAGTGCAGAAAGTCAACGCGGTTTTTTCTATGTTCAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCACGGCCTGGAAAGTGGAAGAAGAGCAATTTATCGTTAAAGCACTGGGGAATGCCTTCGAAGGGCTGTTTGGCAAAgcgc                       <  1:252480/251‑1 (MQ=255)                                                                                                                                                                                                                                     gACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTg                                                                                                                                   <  2:177878/124‑1 (MQ=255)                                                                                                                                                                                                                                     gACCGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTg                                                                                                                                   >  1:177878/1‑124 (MQ=255)                                                                                                                                                                                                                                       ccGTCGTCTGACCTAGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCt  <  1:234494/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             CGTATGGAAATTCGTATTGATGTTCAGGGTGTTAGCTGCCACGGTTCTGCACCAGAACGCGGTGATAACGCCATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCT  >  NC_000913/3009322‑3009802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑