Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,153	T→G	N51T (AAC→ACC)	truD ←	tRNA pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,153	0	T	G	100.0%	111.3 / NA	30	N51T (AAC→ACC)	truD	tRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/11);  total (19/11)

AAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCACG  >  NC_000913/2870913‑2871397                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aggcctttACTTTTCAGCGCGCCTAACCGCAGTTGGCGCTGGGCCCGCGAATACCCCAGCACTGGGCGGCCTTCCAGTGGAAGGGCGCTCAGATGGGGCTTTCCCTTGCCGGGCACGGGAGCGAATAACCGCTGTCCCGTAACAGCATTTTTGTCTTTTGGCCCAGCGAAGCTGCCTTCACGGGCAGAAATTTGCAGGAATTTCGCCGGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGAt                                                                                                                                                                                                                                            <  2:159047/245‑1 (MQ=255)                                                       tCCAGCACCTGGCAGCCTTCCAGTTGAACGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCGCTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTccac                                                                                                                                                                                      >  2:148059/1‑251 (MQ=255)                                                       tCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCGCTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAACGCCCAAATCTTccac                                                                                                                                                                                      >  2:152683/1‑251 (MQ=255)                                                                                aaaaGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTCCCGTAACAGCATTTTTGTCTTTTTGCCCAGCGAAGCTGACTTCCCGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCt                                                                                                                                                             <  1:90560/250‑1 (MQ=255)                                                                                       gcTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagc                                                                                                                                                       >  1:42172/1‑250 (MQ=255)                                                                                       ccTCAGATCGGGCTTTTCCTGGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagca                                                                                                                                                      <  1:173503/250‑1 (MQ=255)                                                                                                                     gcgAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGCAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTCTTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAACAGCCCGGTGCCTTGCGGTTTACCGTGGAGGt                                                                                                                        >  1:139527/1‑251 (MQ=255)                                                                                                                      ggAGCGCATACCCCCTGTGCCGAAACAGCATGTTTGTTTTTTTGCCCAGCGAAGCTGATTTCACGGGCATGAATTTACGGGAATTTCGCCGGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTa                                                                                                                       <  2:75821/250‑1 (MQ=255)                                                                                                                             aTAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGAtt                                                                                                                                                                                                                                           >  2:149491/1‑128 (MQ=255)                                                                                                                             aTAACCACTGTCCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGAtt                                                                                                                                                                                                                                           <  1:149491/128‑1 (MQ=255)                                                                                                                                        tCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTc                                                                                                     >  1:7325/1‑251 (MQ=255)                                                                                                                                           gTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTCCCCGGGCATGAATTTTCAGGAATTCCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                                  <  1:229773/251‑1 (MQ=255)                                                                                                                                                aGCATGTTTGTCTTTTTCCCCAGCGAAGTTGACTTCCCGGGCATGAATTTTCAGGAATTTCGCCGGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAttt                                                                                             <  1:105577/251‑1 (MQ=255)                                                                                                                                                     gTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTttgttg                                                                                        >  2:254801/1‑251 (MQ=255)                                                                                                                                                           cTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTa                                                                                   >  2:223213/1‑250 (MQ=255)                                                                                                                                                                              gcctacACGGGCATGAGTTTTGAGGAATGGCGCCAGGGCAGCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCATGCTCAAAGCCCAAATCTTCCACCTCCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTTCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTGTGTTGCCTTAATGAGTAGCGCCACCGCTTc                                                               <  1:286003/246‑1 (MQ=255)                                                                                                                                                                                cTTCACGGGCCTGCATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAAAGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTcac                                                             >  2:45676/1‑251 (MQ=255)                                                                                                                                                                                  tCACGGGAAGGAATTTTCAGGATTTTCGCCAGGGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTcaca                                                            <  2:175941/250‑1 (MQ=255)                                                                                                                                                                                   cACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTGACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAgg                                                          >  2:150820/1‑251 (MQ=255)                                                                                                                                                                                          aTGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAgcg                                                                         >  1:168509/1‑229 (MQ=255)                                                                                                                                                                                          aTGAATTTTCAGGAATTTAGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATccc                                                   >  1:50881/1‑251 (MQ=255)                                                                                                                                                                                           tGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCt                                                  >  2:39001/1‑251 (MQ=255)                                                                                                                                                                                                       aaTTTCGCCAGCGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGAAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCg                                      >  2:181086/1‑251 (MQ=255)                                                                                                                                                                                                                           cacaAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                  >  2:177931/1‑251 (MQ=255)                                                                                                                                                                                                                            acaAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                  >  1:65818/1‑250 (MQ=255)                                                                                                                                                                                                                             caAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgtag                <  1:255941/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGc                                                                                       >  2:270548/1‑176 (MQ=255)                                                                                                                                                                                                                                 aCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGc                                                                                       <  1:270548/176‑1 (MQ=255)                                                                                                                                                                                                                                     gaaTTGCAGCCGGGTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGATCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCAACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCt        >  2:27932/3‑251 (MQ=255)                                                                                                                                                                                                                                           cAGCCGGTTTTGAGGATTCTAACCCGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCCCGTCCGGTAACTCCCAGTTTTTCCGTAGTAGTGGCTTTcccg  >  2:193407/1‑248 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     AAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCACG  >  NC_000913/2870913‑2871397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑