Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,288	C→T	G161D (GGC→GAC)	recA ←	DNA recombination/repair protein RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,288	0	C	T	100.0%	88.8 / NA	29	G161D (GGC→GAC)	recA	DNA recombination/repair protein RecA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/11);  total (18/11)

CTACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCAC  >  NC_000913/2823048‑2823532                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     cTACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGcc                                                                                                                                                                                                                                            >  1:305378/1‑251 (MQ=255)      caccacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCTTACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCg                                                                                                                                                                                                                                        >  2:211924/1‑250 (MQ=255)                                         gacgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCa                                                                                                                                                                                                    <  1:221279/251‑1 (MQ=255)                                             gATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAacg                                                                                                                                                                                                 >  2:28559/1‑250 (MQ=255)                                                         aaCAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCt                                                                                                                                                                                     >  2:259581/1‑250 (MQ=255)                                                                gCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGcc                                                                                                                                                                             >  1:277473/1‑251 (MQ=255)                                                                  gTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGcc                                                                                                                                                                             >  2:239342/1‑249 (MQ=255)                                                                      aaTTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGATTCAACGACGATAACGTCTACTGCGCCAGAAcg                                                                                                                                                                       <  1:98767/251‑1 (MQ=255)                                                                             gcgcGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAgg                                                                                                                                                                >  2:53942/1‑251 (MQ=255)                                                                                      caccGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTATTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTcac                                                                                                                                                         >  2:148807/1‑249 (MQ=255)                                                                                          ggTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGCTGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGAttt                                                                                                                                                   >  2:262872/1‑251 (MQ=255)                                                                                                                 aCATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTcc                                                                                                                            >  2:69007/1‑251 (MQ=255)                                                                                                                    tCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGc                                                                                                                         >  2:95040/1‑251 (MQ=255)                                                                                                                                       gATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGt                                                                                                      >  2:250563/1‑251 (MQ=255)                                                                                                                                                         aagcagcGGGGTGGACGGGTTCAGGTTACCCGCCAGCGTACGCATCGCCTGGCTCATCAGACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACGGCGCCAGAACGCGCCAGGGCGGCACAGATTGCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACATCAGGTTGTCGATATCGACGCCCAGtt                                                                                    <  2:187274/250‑1 (MQ=255)                                                                                                                                                           gcagcGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTa                                                                                  <  1:148807/251‑1 (MQ=255)                                                                                                                                                            cagcGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTAc                                                                                 >  1:131442/1‑251 (MQ=255)                                                                                                                                                                                       cGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGtt                                                      <  1:69007/251‑1 (MQ=255)                                                                                                                                                                                                aCGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACCGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCg                                              >  2:247798/1‑250 (MQ=255)                                                                                                                                                                                                 cGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGAt                                            >  2:98688/1‑251 (MQ=255)                                                                                                                                                                                                      cGCCGGGCTCATCATACGGGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGAGAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACg                                       <  2:157067/251‑1 (MQ=255)                                                                                                                                                                                                        ccTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCg                                                                                                                                                                                                                           >  2:124012/1‑69 (MQ=255)                                                                                                                                                                                                        ccTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCg                                                                                                                                                                                                                           <  1:124012/69‑1 (MQ=255)                                                                                                                                                                                                          tGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGcac                                    >  1:45114/1‑250 (MQ=255)                                                                                                                                                                                                              tcatcaTACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGttt                                                                                   >  2:212715/1‑199 (MQ=255)                                                                                                                                                                                                              tcatcaTACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGttt                                                                                   <  1:212715/199‑1 (MQ=255)                                                                                                                                                                                                                atcatACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGtttt                             <  1:303536/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGctgcgctgcg          <  1:13665/251‑1 (MQ=255)                                                                                                                                                                                                                                           gagTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCAc  <  1:95040/251‑1 (MQ=255)                                                                                                                                                                                                                                             gtcgtcGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAcgctgcgct             >  2:145374/1‑238 (MQ=255)                                                                                                                                                                                                                                             gtcgtcGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAcgctgcgct             <  1:145374/238‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CTACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCAC  >  NC_000913/2823048‑2823532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑