Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,789,286	G→A	G101D (GGT→GAT)	glaH →	glutarate dioxygenase GlaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,789,286	0	G	A	100.0%	102.0 / NA	32	G101D (GGT→GAT)	glaH	glutarate dioxygenase GlaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/12);  total (20/12)

TATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGC  >  NC_000913/2789036‑2789525                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                tataGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCggtggg                                                                                                                                                                                                                                                 >  2:269451/1‑251 (MQ=255)        gATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGCCGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCgat                                                                                                                                                                                                                                          >  2:104322/1‑251 (MQ=255)        gATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCgat                                                                                                                                                                                                                                          >  2:143369/1‑251 (MQ=255)                  cacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGaagc                                                                                                                                                                                                                                <  2:118179/251‑1 (MQ=255)                  cacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGGTTTCGTGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGaagc                                                                                                                                                                                                                                <  1:197190/251‑1 (MQ=255)                        gTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCgg                                                                                                                                                                                                                          <  1:143369/251‑1 (MQ=255)                                       gcgTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGc                                                                                                                                                                                                           <  1:220883/251‑1 (MQ=255)                                              ctgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGAc                                                                                                                                                                                                    >  1:137537/1‑251 (MQ=255)                                                                      acgacCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAAGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCg                                                                                                                                                                            >  1:174231/1‑251 (MQ=255)                                                                         acCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATAGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTc                                                                                                                                                                         >  1:33477/1‑251 (MQ=255)                                                                                         aGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTcgatgcgatg                                                                                                                                                         >  2:266009/1‑251 (MQ=255)                                                                                                   cGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGGTCCAATTTCGATGCGATGAGCGGTCAGt                                                                                                                                               >  2:7830/1‑251 (MQ=255)                                                                                                               gCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGAAGCCAAAATTCTTGACGATCTATGTGCGAACAAGCTGCAACCAATGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGtt                                                                                                                                   >  1:189110/1‑251 (MQ=255)                                                                                                                              cAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGCTGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAACAATg                                                                                                                    >  1:295866/1‑251 (MQ=255)                                                                                                                              aaaaTAGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCACCCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGCCGGTGGCGCCTCTGATTTGCCGTTCCAATTTCGATGCGATGAGCGGTCCGTATTACGCGCGTTTTGTCGTGAAAAATg                                                                                                                    >  1:295872/2‑251 (MQ=255)                                                                                                                                    gTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGAt                                                                                                               >  1:102729/1‑250 (MQ=255)                                                                                                                                     tttCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGtt                                                                                                                                   <  1:40281/229‑1 (MQ=255)                                                                                                                                     tttCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGtt                                                                                                                                   >  2:40281/1‑229 (MQ=255)                                                                                                                                       tCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACt                                                                                                           >  2:60733/1‑251 (MQ=255)                                                                                                                                           cGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGa                                                                                                       <  2:273132/251‑1 (MQ=255)                                                                                                                                           cGTTTGCGGGTTGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGa                                                                                                       <  2:15483/251‑1 (MQ=255)                                                                                                                                                 cGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCt                                                                                                  >  1:239769/1‑250 (MQ=255)                                                                                                                                                              ttCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCg                                                                                    >  1:252845/1‑251 (MQ=255)                                                                                                                                                                      gATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGaa                                                                                                                                                                                                             >  1:67797/1‑122 (MQ=255)                                                                                                                                                                      gATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGaa                                                                                                                                                                                                             <  2:67797/122‑1 (MQ=255)                                                                                                                                                                                cGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTa                                                                  <  2:141957/251‑1 (MQ=255)                                                                                                                                                                                                      gTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTGTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCTCCGCGTGATGGAGCTACACAACGACGGCACGTATGTCg                                            <  2:105198/251‑1 (MQ=255)                                                                                                                                                                                                           aGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAAATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAag                                         >  2:149572/1‑249 (MQ=255)                                                                                                                                                                                                            gACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAgaga                                      <  2:272601/251‑1 (MQ=255)                                                                                                                                                                                                                               gAAGGTGCGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTCGCGACCGCGGTGGCTCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCTACAGCCGCACCGCGTGATGCAGCTACACAACGACGGCACGTATGTCGAAGAGATCACCGATTACGttcttat                   >  2:250393/1‑245 (MQ=255)                                                                                                                                                                                                                                      cGTTGTTGATCAATGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGaaa            <  2:145605/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aaTGCGGTGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGCCGAGc  >  2:48229/1‑250 (MQ=255)                                                                                                                                                                                                                                                 aaTGCGGAGGATGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGc  >  2:239560/1‑250 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                TATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGATGTGAAGCAAGCGGATGAGATGGTGAAGCTGGCGACGGCGGTGGCGCATCTGATTGGCCGTTCCAATTTCGATGCGATGAGCGGTCAGTATTACGCGCGTTTTGTAGTGAAAAATGTCGATAACTCAGACAGCTATCTGCGCCAGCCGCACCGCGTGATGGAGCTACACAACGACGGCACGTATGTCGAAGAGATAACCGATTACGTGCTGATGATGAAAATCGACGAGC  >  NC_000913/2789036‑2789525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑