Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	74.6 / NA	24	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

CCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCG‑ACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTC  >  NC_000913/2173199‑2173682                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           ccGAGATAAGGGTGCGAACCAGATTCCCCCGATGC‑CGGCGGACCGCCACCACCACGAAGAAGCCGTGGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAAAAGCAATTAAAAGGGTGAGTGGGAAAAAAACCAGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGc                                                                                                                                                                                                                                         <  2:289449/251‑1 (MQ=255)   gagaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCG‑ACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                                                                                       >  1:270790/1‑251 (MQ=255)            gTGCGGAACAGATTTCCACGATGCACGGCG‑ACCGCCATAGCCACGAAGAAGCCGATGGGGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATggg                                                                                                                                                                                                                              >  1:32997/1‑251 (MQ=255)                                   cACGGCG‑ACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt                                                                                                                                                                                                       >  2:260008/1‑251 (MQ=255)                                                cATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTg                                                                                                                                                                                           >  1:280492/1‑251 (MQ=255)                                                    gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                                                                                                                       >  2:56815/1‑251 (MQ=255)                                                              gccagatgggggcaatacacctaaaC‑AGCAGCACCTGTTTACACGCCACACAAAGAGTAATTAAAACGGGGAAAGGGAAAAAAATACGGCTTGCCAATACCACC‑‑GCCGTATGTCCCAGCACCAACGCCGGATCAAGGCCAATCAGGAACTCCCGACCGCCGAACTTCTCCTGTTAACGACTACGAGCTGGCTTAGCGATGGCCGTTAAACCAGCCATGATGAGTGTAACCACCCGTGGCATTAGCAGCATc                                                                                                                                                                             <  2:123698/229‑1 (MQ=255)                                                                          aaaGATC‑CCAAACCGGCAGCACCTGATTCCCCGGCACACAAACAGAAATAAAAAGGGTGAGGGGGATAAAAACCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtt                                                                                                                                                                 <  1:260008/250‑1 (MQ=255)                                                                                 cccaaaccgccagcaCCTAATTACCCGGCAAACAAACAGCAATTAAAATGGGGAGGGGGATAAAAACCAGGCTGGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAcc                                                                                                                                                           <  2:250071/241‑1 (MQ=255)                                                                                                         ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GACGTATGTCCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGAcc                                                                                                                                   >  1:191653/1‑250 (MQ=255)                                                                                                                  aaaCAGCAATTAACCTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAAAC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGCGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAccc                                                                                                                          >  2:127307/1‑251 (MQ=255)                                                                                                                             aaaaGGGTGAGTGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGcc                                                                                                               <  1:237345/251‑1 (MQ=255)                                                                                                                                aTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                                                                                            <  1:130027/251‑1 (MQ=255)                                                                                                                                 gggggAGTGGGATAAAAATCAGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATa                                                                                                           <  2:204647/247‑1 (MQ=255)                                                                                                                                                           gCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGCTTAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTACACGCCTTCGAGCTTGCTTAGCGATGGGCGAAAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGATTTTACCGCCAGCTGCCATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAACCCCACGGt                                                                                 >  1:289577/1‑251 (MQ=255)                                                                                                                                                            tgccgatccccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACg                                                                                   <  1:33455/241‑1 (MQ=255)                                                                                                                                                                            gtagttCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCATGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAATCATCCATGATGGGTTTAATCCCCCGTGTCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGTCGATAATCAGCCCCATCACAAAACCCACGGTGTCAGGCTCGCCaaa                                                                  <  1:242397/246‑1 (MQ=255)                                                                                                                                                                                           aaCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACGGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGCAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcg                                                   <  2:155168/251‑1 (MQ=255)                                                                                                                                                                                                         cccAATCAGGAACCCCTGCCCGCCGAACTTCGCCGGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGTTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATTCCGATAATCAGCCCCATCACAAAGCCCACGGTGACAGACTCGCCAAAGTTACCAAATGTGCGCTGAATAtcgtcgt                                     <  2:191653/250‑2 (MQ=255)                                                                                                                                                                                                                       ccTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATTACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTaa                         <  2:29092/249‑1 (MQ=255)                                                                                                                                                                                                                            ccgccgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCttt                                                                                                                                       >  2:219902/1‑134 (MQ=255)                                                                                                                                                                                                                            ccgccgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCttt                                                                                                                                       <  1:219902/134‑1 (MQ=255)                                                                                                                                                                                                                             cgccgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGtt                  >  2:26776/1‑250 (MQ=255)                                                                                                                                                                                                                                   aCTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCt           <  1:137894/251‑1 (MQ=255)                                                                                                                                                                                                                                            gTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTtc  <  2:280492/251‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           CCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCG‑ACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAC‑GGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTC  >  NC_000913/2173199‑2173682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑