Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,498	G→A	V385V (GTC→GTT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,498	0	G	A	100.0%	71.9 / NA	25	V385V (GTC→GTT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

GACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATC  >  NC_000913/2134255‑2134722                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 gACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACctgct                                                                                                                                                                                                                           <  2:109322/251‑1 (MQ=255)      tAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGAcc                                                                                                                                                                                                                       >  1:82201/1‑250 (MQ=255)          aCCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGAc                                                                                                                                                                                                                   <  2:85701/250‑1 (MQ=255)                                                           gAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTAGGCAttgc                                                                                                                                                                 >  2:39553/1‑248 (MQ=255)                                                               gcagcagcaCACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGtt                                                                                                                                                             >  1:192385/1‑251 (MQ=255)                                                                 agcagcaCACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAc                                                                                                                                                           <  2:117836/251‑1 (MQ=255)                                                                    agcaCACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTAcgcg                                                                                                                                                        <  2:192385/251‑1 (MQ=255)                                                                                   gAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGc                                                                                                                                         >  1:68496/1‑251 (MQ=255)                                                                                               ccAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCACTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTc                                                                                                                             <  2:207873/251‑1 (MQ=255)                                                                                               ccAAGGATAATCGAGCCGAGGATAATAAGCCCTTTCGTCGGTTTCAGCACGCCAGGCTGAGTTATTGACGGGTCAACAATGCGCACATCGCCGACGGTGCTGCCCTCGGTGATTTTCAGCTCCGGCTCTTTATTCAGCAGTTGCATATAAACCTGATGACCTGACTCGACATCGCGGGCCAGACGGACAATCTCCCGCTGGGTTTTCGGCATCGCCGATACGCGACCGTGAAggatgacttcttcgccgtc                                                                                                                             >  2:158673/1‑233 (MQ=255)                                                                                                                       aTCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCcagc                                                                                                       >  2:185330/1‑249 (MQ=255)                                                                                                                              cTTTCTTCGGTTTCAGCCCGCCAGGCAGAGTGAATGCCGGGTCAACAATGAGCACATCGCCGACGGCGCCGGCCTCGGTGATTTTCAGCTCCTGCTATTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGCCATCTCGGGTCAGACGGACAATATCCTGCTGGGTTTTCGGAATCGCCGCTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTACAGCAGCGTGCgg                                                                                              >  2:294362/1‑251 (MQ=255)                                                                                                                                ttcttcGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGt                                                                                             <  2:85942/250‑1 (MQ=255)                                                                                                                                     cGGTTTCAGCACGCCCGGCTGAGTGATTGCCGGGTCAACACTGCGCACATCACCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATTGCGGGTCAGACGGACAATCCCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCATTAAGATTGTCTTTTTCGTCTTCCAGCGCCTGACTTTTCTCCAACAGCATGCGGTACGCCg                                                                                       >  2:276484/1‑251 (MQ=255)                                                                                                                                                cGCCAGGCTGAGTGAGTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTGAAGTTTGGCGTTTGCGTCATCCAGCGCCTGACGTTTCGCCAGGAGCGTGCGGTACTCCGGGTGAACTTTg                                                                            <  2:30143/251‑1 (MQ=255)                                                                                                                                                         gaggtaTTGCCGGGTCAACTATGCGCACATCGACGACGTGGCTGGCCTCGGCGATTTTCAGCTCCTGCGCTTTATTCAGCAGTGGCATATAAACCTGGTGACCAGACTCGACATCGCGGGTCAGACGGATAATCTCCGGCTGGGGTTTCGGCATCGCCGTTACGCGACCGTTAAGTGTGGCTTTTTCTTCTTCCAGCGCCTGACGTTTCTCGAGCAGCGAGCGTTACTCTGTGTGAACTTTGGTGTACAGc                                                                   <  2:129210/246‑1 (MQ=255)                                                                                                                                                           gTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCATCGTGCGGTACGCCGTTTGAACTTTGGTGTACAGCtt                                                                 <  2:177571/251‑1 (MQ=255)                                                                                                                                                           gTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCtt                                                                 >  1:73115/1‑251 (MQ=255)                                                                                                                                                                agacggctcaaccaagcgcACATCGCCGACGGTGATGACCCCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCCGTTGCATCTAACCCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACACGACCGTTCAGTTTGGCTTTTTCGTCTTCCAGCGCCTGCCGTTTCTCCAGCAGCGTGCGGCACGCCGGATGATCTTTGGTGTACAGCTTGgagc                                                            >  2:293118/16‑250 (MQ=255)                                                                                                                                                                   cGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGAttt                                                         >  1:231498/1‑251 (MQ=255)                                                                                                                                                                     ggTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTcc                                                       >  1:102469/1‑251 (MQ=255)                                                                                                                                                                                         ccgtgggTGCTGGCCTCGGTGATTTTCAGCTCCTGTTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATAGCGGGTCAGACGGACATTCTCCTGCTGGGTTTTCGGCATCGGCGGTACGCGACCGTGAAGTTTGGCTTTTTCGTCTTCCAGCGCCTTACTTTGCTCCGTCAGCGTGCGTTACGCGGGGTGAGCTTTGGGGTACTGCTTTGATATTTCCGCCTCTCTATagttcagttc                                   <  2:45218/246‑1 (MQ=255)                                                                                                                                                                                                            tGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGTTCAGTTCGTTCAACTGCGCATCGATg                >  2:4204/1‑251 (MQ=255)                                                                                                                                                                                                            tGATTTGCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGCACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATg                >  2:50157/1‑251 (MQ=255)                                                                                                                                                                                                                           gCTCGTTATTCAGAAGTTGAATATAAACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCGCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaatc  <  2:250463/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 GACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATC  >  NC_000913/2134255‑2134722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑