Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,122,908	G→A	I24I (ATC→ATT)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,122,908	0	G	A	100.0%	75.1 / NA	25	I24I (ATC→ATT)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/13);  total (12/13)

CCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCGATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGGAGCGCACTT  >  NC_000913/2122663‑2123154                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ccGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCGATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACAAATAGCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATc                                                                                                                                                                                                                                                   <  2:43089/251‑1 (MQ=255)            cATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGGTGGTGAGGCGGACATCACCGCCTAACAAAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGtt                                                                                                                                                                                                                                        >  2:25426/1‑251 (MQ=255)            cATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGtt                                                                                                                                                                                                                                        <  2:66204/251‑1 (MQ=255)                                                                    tGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGATTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCGGGCAATATCTTCCTTCAGTTCTTCGCCTAATTTCCCGCGCATATCATAGTCTTTACAGCCGGTTAATTTTTTCat                                                                                                                                                                                 >  2:82713/1‑250 (MQ=255)                                                                                           ccGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTGTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATa                                                                                                                                                         <  2:10133/251‑1 (MQ=255)                                                                                              gataTACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGtt                                                                                                                                                      <  1:183805/248‑1 (MQ=255)                                                                                                 aTACCAATATCCAGCACGTCAACGCCCGCATCCTGCAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCCCCGCCTAACCCAATGGTTTTCGGTTTGAGAAATTCGCTATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCCGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTgtc                                                                                                                                                   >  2:210395/1‑249 (MQ=255)                                                                                                                  gTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTAccc                                                                                                                                                                       >  1:101278/1‑214 (MQ=255)                                                                                                                  gTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTAccc                                                                                                                                                                       <  2:101278/214‑1 (MQ=255)                                                                                                                                       aCCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATATGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCGTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCTATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTAttt                                                                                                             <  1:25426/251‑1 (MQ=255)                                                                                                                                                  gcgccagTTTTAAGGTTTCGCTGGTGAGGCGGACATCACAGCCTACAACAATGGCTTTCGGTTTGAGAAATTCGCCATAGGCGAGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGAAATAGTTGGCCATGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGtt                                                                                                  >  1:49227/1‑251 (MQ=255)                                                                                                                                                         ttttAAGGTATCGCTGGTACGGCGTACATCACCGCCTAAACCACTGGTTTTCGGTTTGAGCAATCCTCCACCGGCGCGACCACTTCGCCATGCACTATCTTACTTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTCATTTTTTCCTATCGTTACCCTTTTTCAGGCAATCGCTGGCCCTTCACGAAGCGTCACATTTTATTTTTTCCGTTATTTacacgtcggtatcgtatt                                                                                           >  2:30536/1‑237 (MQ=255)                                                                                                                                                           ttAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGGCGTTATTTACACCGCGGTTTCGcattc                                                                                          <  1:113912/250‑1 (MQ=255)                                                                                                                                                                                               acacAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCcgc                                                                                                                                                                                                                      <  1:31978/90‑1 (MQ=255)                                                                                                                                                                                               acacAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCcgc                                                                                                                                                                                                                      >  2:31978/1‑90 (MQ=255)                                                                                                                                                                                                          ttCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACccg                                                                <  2:123397/229‑1 (MQ=255)                                                                                                                                                                                                          ttCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACccg                                                                >  1:123397/1‑229 (MQ=255)                                                                                                                                                                                                            cGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACGCCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGcac                                        <  1:118858/251‑1 (MQ=255)                                                                                                                                                                                                                           gCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTc                         >  1:89667/1‑251 (MQ=255)                                                                                                                                                                                                                               tagccgcgACCAAGGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCATGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCg                        >  2:85214/5‑248 (MQ=255)                                                                                                                                                                                                                                      gACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGc              <  2:217428/251‑1 (MQ=255)                                                                                                                                                                                                                                      gACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGc              <  1:163612/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGGAgcgccct   >  2:49829/1‑248 (MQ=255)                                                                                                                                                                                                                                                 cAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGGAGCGCACt   >  1:73033/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGGAGCGCACtt  <  1:76161/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCGATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGGAGCGCACTT  >  NC_000913/2122663‑2123154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑