Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,085,319	G→A	A69A (GCC→GCT)	tsuA ←	thiosulfate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,085,319	0	G	A	100.0%	25.6 / NA	10	A69A (GCC→GCT)	tsuA	thiosulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

AATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGA  >  NC_000913/2085209‑2085557                                                                                                               |                                                                                                                                                                                                                                               aaTCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACg                                                                                                    >  1:239370/1‑251 (MQ=255)      cccccccccccattACCAGGTCCCGGGGGCAACCCCACCCGCCAGAACAATTCCCAGCCCGAAGATATCCCCCCCTATAACAGTTCCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCAc                                                                                               <  2:81121/238‑1 (MQ=255)            cgcgcgATACCAGGTCCCGGTGGCACATCCACCCGCCAGCACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGACATTGCAATCAGCACGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGc                                                                                          >  1:23660/1‑250 (MQ=255)                                                  gAACAATGCCCATCCCGAAGATATACCCACCTATAACAGTACCAATCCACGGGAACGCCACAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCGTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACAAATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTTCATGACAAATCCCATTAGAGCACCCCAAATTAGCCCGCtt                                                   <  2:21226/251‑1 (MQ=255)                                                            cAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTg                                         >  2:78329/1‑251 (MQ=255)                                                               cccGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGAAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTgca                                       >  2:12339/1‑248 (MQ=255)                                                                                     acattACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGATCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATg                <  1:129997/247‑1 (MQ=255)                                                                                                  aCGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGcac                                                                    <  1:85311/186‑1 (MQ=255)                                                                                                  aCGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGcac                                                                    >  2:85311/1‑186 (MQ=255)                                                                                                    gggAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGCATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGa  >  1:111893/1‑250 (MQ=255)                                                                                                               |                                                                                                                                                                                                                                               AATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATGTTAATGAGGA  >  NC_000913/2085209‑2085557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑