Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,084,330	C→T	A42T (GCA→ACA)	tsuB ←	putative thiosulfate sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,084,330	0	C	T	100.0%	43.9 / NA	14	A42T (GCA→ACA)	tsuB	putative thiosulfate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

TTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGC  >  NC_000913/2084094‑2084564                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           ttGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCa                                                                                                                                                                                                                               >  2:164832/1‑250 (MQ=255)  tGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCaa                                                                                                                                                                                                                              >  2:227664/1‑250 (MQ=255)                                    agagAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCt                                                                                                                                                                                           <  2:98652/251‑1 (MQ=255)                                                          gCACTTTGGCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAAACGGTGATGGCATGTACTTCTTCTTCCGCCCCCTGTGGGATTGCTTCAGTTGTCTGGGGGCAATCAAACTCAATCACTAACTCATCGCCGCTGACATTTTCTGCCAGAGCTGCTTTTGCTTCt                                                                                                                                                                      >  2:167419/1‑250 (MQ=255)                                                                 ggCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATATTCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTgg                                                                                                                                                              <  2:69273/251‑1 (MQ=255)                                                                           ggAAGAGATTGTCTAATTTGACGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGCACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTTCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGAGGAAACGGAc                                                                                                                                                      >  2:202064/1‑249 (MQ=255)                                                                                          aTTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTcaccac                                                                                                                                      >  1:306144/1‑250 (MQ=255)                                                                                                        ccgcTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAAt                                                                                                                       <  2:302142/251‑1 (MQ=255)                                                                                                                                                 aaCGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGcc                                                                              >  1:199105/1‑251 (MQ=255)                                                                                                                                                          gCTCCACGCTGCATCGCCAATCGGCTGATAATCGGTGATGGCATGTCCTGCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCGTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCAGCTTTACACCGTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGcc                                                                     <  1:135482/251‑1 (MQ=255)                                                                                                                                                             ccACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGCCt                                                                    <  1:45734/249‑1 (MQ=255)                                                                                                                                                                                  ctgATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATacac                                              >  2:297066/1‑250 (MQ=255)                                                                                                                                                                                            gTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACg                                   <  2:306144/251‑1 (MQ=255)                                                                                                                                                                                                                             gggATTGCTTCAGTTGTCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGc  <  1:243229/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           TTGCAGATGCTGGTACAACAATATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAATTTGAAGCCTCCGCCGCTGGTACGGCGGAGGACAATGTTGAGTCAGGCTTTTTGAACGGTGATGCTCCACGCTGCATCGCCAATCTGCTGATAATCGGTGATGGCATGTCCTTCTTCTGCCGCCCACTGTGGGATTGCTTCAGTTGCCTGGGTGCAATCAAACTCAATCACTAACTCATCGCCGCTGACCATTTCTGCCAGAGCTGCTTTTGCTTCTATGAGTGGAAACGGACAGACCTGCGTCACCACATCCAGCTTTTTAATCACCATCTTTACACCTTACTTAATTTGCCGCAGCAGTTGCCAGTCGCGCCTTACGCTGCGGTCGAACATACACAAGCCAGGACGCAGTCCAGACTCCGAGAATCATAAATACAAGGC  >  NC_000913/2084094‑2084564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑