Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,080,405	C→T	G63S (GGT→AGT)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,080,405	0	C	T	100.0%	43.0 / NA	15	G63S (GGT→AGT)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/8);  total (7/8)

CGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCATCT  >  NC_000913/2080190‑2080634                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      cGGGGACCCCAAAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATg                                                                                                                                                                                                     <  1:83750/250‑1 (MQ=255)                          gTCACCCCAATCAATATACCTTGATACGGTTTCTTGCCCCGCGCCAGCAAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAACTCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCgg                                                                                                                                                                           >  2:305209/1‑251 (MQ=255)                                                         tCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGACAAGTg                                                                                                                                            >  2:300810/1‑251 (MQ=255)                                                                                             ccgccgCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGAc                                                                                                                                                                                                >  1:112815/1‑163 (MQ=255)                                                                                             ccgccgCGCACCAGACAAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGAc                                                                                                                                                                                                <  2:112815/163‑1 (MQ=255)                                                                                                      aCCAGACTAACATCAGCGGTACCGAGATTAACTCCAGTGGCAGAGCGATAAGACCTAAAATCGACCCCACCACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGAATGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTaa                                                                                               <  2:215767/251‑1 (MQ=255)                                                                                                                  tCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATAcc                                                                                   >  1:75862/1‑251 (MQ=255)                                                                                                                  ccAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATAcc                                                                                   <  1:103933/250‑1 (MQ=255)                                                                                                                         aaaCGAGAGTAACCCCAGTTGCAGAGGCATAAGACCTAAAATCGAACCCAGCCCCCTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGAttt                                                                            <  2:82190/250‑1 (MQ=255)                                                                                                                            cGAGATTAACTCCAGTTACAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAACGGCGCGAGGGACAACGTTACTCCAGACCGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCAAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGCGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCAACATGCGGTAATGGCGGTGTACCCCGGTTTCa                                                                          >  2:44670/1‑250 (MQ=255)                                                                                                                                            ggCAGAGCGTGAAGACCTACAATCGAACCCAACCCCTTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATTACCGGATTTCAATCGGGCTTAATGACt                                                          <  2:173338/249‑1 (MQ=255)                                                                                                                                                               aaaTCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGaaa                                      <  1:159519/251‑1 (MQ=255)                                                                                                                                                                         cAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAAtt                            >  1:220143/1‑250 (MQ=255)                                                                                                                                                                                  acagCCAATACGCTCAAAGGCGCGAGGGACAACGTTACTCCCGAACGAGATTGGACCCATAATCACCACCATGGTGACCAGCGTCCAGGATCTTTCCGGGATAGGAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAAGGGCGGTAAAACCGGATTTCAAAAGGGCATAATGACATATTGGACCGCACGCAGaaaaaccatcattagattaaa                    >  2:109189/4‑232 (MQ=255)                                                                                                                                                                                                   aGGCGCGAGGGACAACGTTACTCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCATCt  <  2:274421/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      CGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTATCGGCACGCACGCAGAAACTCCGTAATGAGATTAAAAGCGCTCATAACTCATCT  >  NC_000913/2080190‑2080634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑