Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,955,010	G→A	D666D (GAC→GAT)	torZ ←	trimethylamine N‑oxide reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,955,010	0	G	A	100.0%	44.9 / NA	15	D666D (GAC→GAT)	torZ	trimethylamine N‑oxide reductase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

TCATGCACGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCA  >  NC_000913/1954765‑1955245                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            tCATGCACGCATACCAGGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATcggc                                                                                                                                                                                                                                        >  2:256718/1‑251 (MQ=255) tCATGCACGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCtcatcg                                                                                                                                                                                                                                          <  2:301787/249‑1 (MQ=255)          cATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCttt                                                                                                                                                                                                                               >  1:225916/1‑251 (MQ=255)                                                    gTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATc                                                                                                                                                                                     <  1:44587/251‑1 (MQ=255)                                                      cAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATcc                                                                                                                                                                                    <  2:54185/250‑1 (MQ=255)                                                                                                                                     aTAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCCATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAg                                                                                                                                                   >  1:177823/1‑204 (MQ=255)                                                                                                                                     aTAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAg                                                                                                                                                   <  2:177823/204‑1 (MQ=255)                                                                                                                                                         ggCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTTATCAGGAGCCAGCCAGGGTGGGTGTGCCGGGCAAGCCTTATAGCCAAATTTTTCCAACCTTTTTGGATAGTTTTCAATTTTGCCGCTTGTCGTACCCCGCGCATTTTTCACAGGATCGGCGCGGAAATCCCCATaac                                                                                >  1:170558/1‑251 (MQ=255)                                                                                                                                                                gCATATTTTTTCCGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTTCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGt                                                                          >  1:223070/1‑250 (MQ=255)                                                                                                                                                                gCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCCTAACGAACGt                                                                          >  1:115760/1‑250 (MQ=255)                                                                                                                                                                                                  gACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCAACCGGAGCCAGCAAGGGTGGGGGTGGCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTTAATTTTTCCGCTTGGGGTACCCAGCGCATTTTTTACCGGATCGGCGCGGAAATCAACATAACGAAAGTACTGTTTGTTCCTTTTGCTGGGGCGCGTTTCggtt                                       >  1:145961/1‑248 (MQ=255)                                                                                                                                                                                                         tAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATTGCCAAATTTTTCCAACGTTTTTTAATAGATTTTAATTTTTCCGCTTGGCGTAACCCGCGCATTTTTCACCGGATCGGCGCGGAAATCACCCTAACGAACCTACTTTTCTTTCTTCTCGCTGTGgcggctttcgctccgttttt                                >  2:116421/1‑234 (MQ=255)                                                                                                                                                                                                                 gtgCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTtgctgc                        <  1:35900/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAGAAGCTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAAc         <  1:224137/250‑1 (MQ=255)                                                                                                                                                                                                                                    gaGCTGGAACCGCTTCTCAGCGGCGGTAACTTTCCATTCCGCAGGAGGCAGCCAGGTTGGGTGTGCCGGGCAATCCGTATAGCCAAAATTTTCCAACGTTTTTGAAGAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCCCCGGATCGGCGCGGAAAGCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTAGCGCATTTCGGTCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTg     <  2:54696/250‑1 (MQ=255)                                                                                                                                                                                                                                       cTGTAACTGCTTCTCATCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGGGCCGGGCAATCCTTTTAACCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGGAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGGGGCGCATTTTGATCAGTTTTTTTTTCTTCCAGAAGGCCTTTAACATTGGcc  >  2:149884/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            TCATGCACGCATACCACGCCTTTTTTGATCCCGTCAGTCACCACCGCGCCTGTCAAAATCTGACCACGTTTGTTCCAGACACGCACCAGATCGCCATTCGCAATACCAAAGCGTGCAGCATCTTCGGTGTGAATAGTGATTGGTTCACGATCGGCGATCGCATATTTTTTACGCAGTTCCGCATAGTTAAGCTGACTGTGTAAACGGTGTGCCGGATGTGCGGTCAGAAGCTGTAACTGCTTCTCGTCGGCGGTACCTTTCCATTCATCAGGAGCCAGCCAGGTTGGGTGTGCCGGGCAATCCTTATAGCCAAATTTTTCCAACGTTTTTGAATAGATTTCAATTTTGCCGCTTGGCGTACCCAGCGCATTTTTCACCGGATCGGCGCGGAAATCACCATAACGAACGTACTGTTCGTTCTTCTCGCTGTGGCGCATTTCGATCAGTTTATTTTGCTGCCAGAAGGCATTAAACATTGGCA  >  NC_000913/1954765‑1955245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑