Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,824	C→T	A567T (GCA→ACA)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,824	0	C	T	96.7%	82.2 / NA	30	A567T (GCA→ACA)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (15/14);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACC  >  NC_000913/1948584‑1949067                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGtcatc                                                                                                                                                                                                                                           <  2:284743/251‑1 (MQ=255)            aaGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCt                                                                                                                                                                                                                                  <  1:154319/249‑1 (MQ=255)                tCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGTGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTgccgcc                                                                                                                                                                                                                            >  2:215793/1‑251 (MQ=255)                  gaaccGGGACGCTTATACTCTTTATCCTTCGCCCGGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGGTTCATATCAGTTATTCTCAGCCTTCTTCCCAACCGGAATGCTCAGCTCAGCCAGTGCAGGCGGGTTAGCAGGGCTCTGTGTTTCAGTCTTCAGACATGCGGCCGCCGt                                                                                                                                                                                                                          <  2:308035/247‑1 (MQ=255)                   aaaCGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTg                                                                                                                                                                                                                         >  1:193615/1‑251 (MQ=255)                         gACGCTTATACAATTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCCGTCATCAGACACGCTGCCGCCGTGGTTTTc                                                                                                                                                                                                                   >  1:64007/1‑251 (MQ=255)                                  acacTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATGTCAGTTATTCTCAGCCTTCTTCACACCCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCAGCCGTGGTTTTCGGGAAGGCg                                                                                                                                                                                                          >  2:58599/1‑251 (MQ=255)                                                     gCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTg                                                                                                                                                                                        <  1:40395/250‑1 (MQ=255)                                                          ttcgtcgccTGCCTTCGCTCATACCATTCCCATAGATATATATGCTCATGAAAGCTCACTCAGTTGCCGCCTCGATGCAACGCGACTGATATCGTGTCTTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTg                                                                                                                                                                                  >  2:273663/9‑251 (MQ=255)                                                                          gCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATgg                                                                                                                                                                  >  1:35154/1‑251 (MQ=255)                                                                                      cacaTAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCaa                                                                                                                                                      <  2:99830/251‑1 (MQ=255)                                                                                        caTAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGGCAAGa                                                                                                                                                    <  1:215793/251‑1 (MQ=255)                                                                                                                            ccgccTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTAt                                                                                                                >  1:178661/1‑251 (MQ=255)                                                                                                                                cTCGATGCAACGCGAATGATTTCGTGGATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACCGATATTGTCGGTGCCGGTCCGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTc                                                                                                             >  2:125843/1‑250 (MQ=255)                                                                                                                                        aaCGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGCGCGTCg                                                                                                    >  2:259632/1‑251 (MQ=255)                                                                                                                                         aCGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCg                                                                                                    >  1:2316/1‑250 (MQ=255)                                                                                                                                                  aTTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGc                                                                                          <  1:125843/251‑1 (MQ=255)                                                                                                                                                                         aGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGGTCctct                                                                   >  2:116148/1‑251 (MQ=255)                                                                                                                                                                             aTTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCg                                                                <  1:231697/250‑1 (MQ=255)                                                                                                                                                                                   aGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATAc                                                         <  1:7826/251‑1 (MQ=255)                                                                                                                                                                                           tcACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTCCTCGCGCTGTTCCTCTTCGTTGATACCCAGact                                                  >  2:169341/1‑248 (MQ=255)                                                                                                                                                                                             ccatccTGAATGCTCAGCTCCGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACTCGCTGCCGCCGTGGGTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACGTGCGTGCGGCGGAGTACCGGATTTCAGAGCTTCGAGCAGGAAGCCGAAGTTCTCGCGCTGGTCCTCTTCGTTGATGCCCAGAATAcc                                               <  2:36063/247‑1 (MQ=255)                                                                                                                                                                                              caACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATAcc                                               >  2:80492/1‑250 (MQ=255)                                                                                                                                                                                               aaCCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCaa                                             <  2:2316/251‑1 (MQ=255)                                                                                                                                                                                                   tGAATGCTCCGCTCAGCCAGTGCAGTCGGGTTAGCCACGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGACTGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGGATTTCTCGCGCTGTTCCTCCTCGTTGGTACCCAGAATACCAAaccc                                         >  2:146649/1‑249 (MQ=255)                                                                                                                                                                                                     aaTGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCg                                       >  1:139978/1‑251 (MQ=255)                                                                                                                                                                                                           cagctcagcCAGTGCAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGATCTGCGTGCGGCGGAGTACCGTATTCCAGAGCGTCGAGCAGGAAGCCGAATTTCGCGCGCTGTTCCTCTTCGTTGATACCCAGAAGACCAAACACCGTctgc                                  <  2:153668/250‑1 (MQ=255)                                                                                                                                                                                                                         cAGTCGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTa                   <  2:112255/251‑1 (MQ=255)                                                                                                                                                                                                                             cGGGTTAGCAAAGCTCGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATgg                >  1:67839/1‑250 (MQ=255)                                                                                                                                                                                                                                            cGGTGTTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAacc  <  2:193615/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    AGATGACCACTAAGATCGAAACGGGACGCTTATACACTTTATCCTTCACGCTGCCTCTTCGTTGACTGCCTTCGCTCATCCCATTCACATAGTTATCTATGCTCATGGGAGTTCACTCAGTTGCCGCCTCGATGCAACGCGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACC  >  NC_000913/1948584‑1949067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑