Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,906,796 C→T V89M (GTG→ATG)  rlmA ← 23S rRNA m(1)G745 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,906,7960CT95.8% 71.9 / NA 24V89M (GTG→ATG) rlmA23S rRNA m(1)G745 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/13);  minor base G (0/1);  total (10/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCAC  >  NC_000913/1906551‑1907020
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                
gggCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTAGCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTTGCCTGCGGATAGCGTTTCGCGGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCGCAGCCAATATCCAGGAtcgccg                                                                                                                                                                                                                             <  2:114248/251‑1 (MQ=255)
                   aaTTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACAGCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGcc                                                                                                                                                                                                           <  1:54735/250‑1 (MQ=255)
                          ctGCTTTACACGGCGCGTAAATACGTATTATGGCATCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTc                                                                                                                                                                                                   >  1:232996/1‑251 (MQ=255)
                            gCTTTACCCGGCGCTTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTc                                                                                                                                                                                                   <  2:22617/249‑1 (MQ=255)
                                                  gTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGAAACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGc                                                                                                                                                                           >  1:140119/1‑251 (MQ=255)
                                                        tGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGc                                                                                                                                                                     >  1:121136/1‑251 (MQ=255)
                                                                     ggTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCgg                                                                                                                                                        >  1:209181/1‑251 (MQ=255)
                                                                                      aaCGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCATTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATACTGTCCGGCATCTAAGAATGCgcgg                                                                                                                                       >  2:228989/1‑251 (MQ=255)
                                                                                         ggTGGCTGGAAGCAACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACAGCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTGATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGt                                                                                                                                    <  2:215584/251‑1 (MQ=255)
                                                                                          gTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTg                                                                                                                                   <  2:195957/251‑1 (MQ=255)
                                                                                                   aGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCGCCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGcatca                                                                                                                          <  2:209030/251‑1 (MQ=255)
                                                                                                              aaGTGACCTGCGGATAGCTTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTTTGTTTAATACCCTTCACCACAGCCAATATCCAGCAGCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGTAGCGGCTAAGAATGTCCGGCAGCTAAGAATGCGTGGCGTGCGTGGATCATTTTCGCGCTg                                                                                                               <  2:190699/251‑1 (MQ=255)
                                                                                                                                 tttCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGAcc                                                                                            >  1:288108/1‑251 (MQ=255)
                                                                                                                                      ccgccgcTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTa                                                                                       <  1:57754/251‑1 (MQ=255)
                                                                                                                                        gccgcTTGTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATg                                                                                     <  1:274799/251‑1 (MQ=255)
                                                                                                                                                            cGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTg                                                                  >  1:269138/1‑250 (MQ=255)
                                                                                                                                                                               gtggtgATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCat                                              <  2:121136/251‑1 (MQ=255)
                                                                                                                                                                                   tgATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATc                                           >  1:64648/1‑250 (MQ=255)
                                                                                                                                                                                           ggCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCCTCAACCCGTTCCCTCAGTTGGGCGACTATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCCGATTGACATCCCCTTCTTTCGCCATATCAAACTGATg                                  >  1:47741/1‑251 (MQ=255)
                                                                                                                                                                                                cGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCt                             <  1:17245/251‑1 (MQ=255)
                                                                                                                                                                                                      ggCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACCATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGAc                       >  2:275440/1‑251 (MQ=255)
                                                                                                                                                                                                          aaTGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTTGGGACAGat                   <  1:257959/251‑1 (MQ=255)
                                                                                                                                                                                                            ggCGTGTGTGTAATCCCCTTCACCCCAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGatat                 <  2:894/250‑1 (MQ=255)
                                                                                                                                                                                                                     gtAATACCCTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGttt         <  1:229893/250‑1 (MQ=255)
                                                                                                                                                                                                                           cccTTCACCACAGCCAATATCCAGCATCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCAc  <  2:269138/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                
GGGCTTCACTACTCGTGCTAATTCTTCTGCTTTACACGGCGCGTAAATACGTATTATGGCGTCCATACTGGTATCGGAAAACGGCAAACGGTGGCTGGAAGCGACACAAAAAGTGACCTGCGGATAGCGTTTCGCCGCCGCTTTTATCGCTACCTTCGAAACATCCAGACCAAACGTGGTGATTTCGGGCAACGCATCGGCAAATGCGTGTGTGTAATACCCTTCACCACAGCCAATATCCAGCACCGCCGTGGCCTTATCATCAAGCCGTTCCCTCAGTTGGGCGACAATTGCATCACGCAGCGGCTGATAATGTCCGGCATCTAAGAATGCGCGGCGTGCTTGCATCATTTCCGCGCTGTCGCCCGGATCACGAGACCGTTTATGCTGAACGGGCAGCAGATTGACATACCCTTCTTTCGCCATATCAAACTGATGTCGCTGGGGACAGATATAGCTGTTTTTTTCAC  >  NC_000913/1906551‑1907020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑