Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,878,855	C→T	A28V (GCT→GTT)	yoaK →	putative membrane protein YoaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,878,855	0	C	T	100.0%	13.6 / NA	6	A28V (GCT→GTT)	yoaK	putative membrane protein YoaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

TCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAAT  >  NC_000913/1878715‑1879045                                                                                                                                             |                                                                                                                                                                                               tCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTaaaaaa                                                                                   >  1:164006/1‑250 (MQ=255)                                      aTCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGAACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTa                                             <  1:217532/251‑1 (MQ=255)                                            ttGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATTGTTTTTTATTGGCGGCTATGTTGGCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTTTCGTAATCGGCACTGAGCTGGGATGGTGGTAACGTCAACTCTAAAAAATAGCAAAAGCTGCCTGTGTGCAGCCTTTTTGCAAATTAAGCGtt                                       >  2:154151/1‑251 (MQ=255)                                              gATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTTCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGTGGGTAACGTCACCTCTAAAAAATAGCAAAGGCTTCCTGTGTGCAGCCTTTTTGCAATTTAAGCGTTa                                      >  2:104171/1‑250 (MQ=255)                                                         aCATGCGAATCAGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGTTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGGGCAATTTAAGCGTTAACTTGTAATCtt                          <  2:189932/251‑1 (MQ=255)                                                                                 cGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGTTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAAt  <  1:126761/251‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                                                               TCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGATGAAAAAAACAACGATTATTATGATGGGTGTGGCGATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAAT  >  NC_000913/1878715‑1879045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑