Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,876,099	C→T	L143L (CTG→TTG)	nimT →	2‑nitroimidazole exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,099	0	C	T	100.0%	64.6 / NA	22	L143L (CTG→TTG)	nimT	2‑nitroimidazole exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

CACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCA  >  NC_000913/1875869‑1876339                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 cACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGc                                                                                                                                                                                                                                  <  2:192742/247‑1 (MQ=255)                                  gTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAAAGCGGTTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGGTCGGCTATGGTCGTGCCGCTGGcc                                                                                                                                                                                             >  2:94961/1‑250 (MQ=255)                                                                     aTCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTTCATTTTGCAATGTCTTACTGCCAGGATTTATTAAACGCGATTTCCCTCTTTCCCTCGCCCGACTTCACGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCCCTGGGAGCGGCCCTGGTCTGGCCGCAGGCTTTTAACGGTTTTTGCTGGCACAGCGCGtttgcctc                                                                                                                                                          >  2:111591/1‑245 (MQ=255)                                                                                                                tattatTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGcc                                                                                                                <  1:251009/250‑1 (MQ=255)                                                                                                                  ttattTGGCGGTACAGCGGTCATTGGCGGTGGGCTTGCATTTGGCAATCTCTTACCGGCCGGATTTATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTTCTTGACAATGGGAGCTGCAGCgg                                                                                                                                                                                                                             >  1:259506/1‑139 (MQ=255)                                                                                                                  ttattTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCAGTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCgg                                                                                                                                                                                                                             <  2:259506/139‑1 (MQ=255)                                                                                                                        ggCGGTACAGCGGTCATTGGCGGTGGGGTTGCATTAGGCAATGGCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCCTTCCGTCGCCCGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCCAGGCGCGGTGCTCATGCTGGTGTGTTTTTCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTgggg                                                                                                       >  1:262243/1‑249 (MQ=255)                                                                                                                                    gTCATTGGCGGTGGGATTGCATTAGGCAAGGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGTAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCGGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAacaaca                                                                                           <  2:42452/251‑1 (MQ=255)                                                                                                                                                  gATTGCATTAGGCAATGTCTTACTGGCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGAATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGGCACAGTGGCGAAGTCCACACCATGCAAATTTGAGt                                                                              >  2:82529/1‑250 (MQ=255)                                                                                                                                                  gATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGATTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTAACCAACATCCAAAtttacgt                                                                              >  2:237252/1‑246 (MQ=255)                                                                                                                                                                 tGTCTTACTGCCAGGATTAATTAAACGCGCTTTCCCTCATTACGCCGCCAGACTTACCGGCGCATATTCCTTGAAAATGGGAGCTCCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGATTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCTCGCGCCTTAc                                                              >  2:140218/1‑251 (MQ=255)                                                                                                                                                                  gTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATGCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGTAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACa                                                             <  2:55377/251‑1 (MQ=255)                                                                                                                                                                   tcggaaggaaaGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACAt                                                            >  1:299457/11‑251 (MQ=255)                                                                                                                                                                     ttACTGCCAGGATTAATTAACCGCGCTTTCCCTCCTTCCGTCGCCAGACTTACCGGAGAATATTCCTTGACAATAGGAGCTGCAGCCGCACTGGCATCGGCTATGGTCGTGCCGCTGGATTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCATCTGCTGGCTCTTTTTTTATAGCTGCCACAGTGGCGAAGTCAACCACATGAACATTTGAGTACCTCGCGCGCCTTACATAc                                                          >  2:137893/1‑251 (MQ=255)                                                                                                                                                                              ggATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTAt                                                 >  1:147694/1‑251 (MQ=255)                                                                                                                                                                              ggATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCGCGCGCGCCTTACCTACTCGGGGTAt                                                 >  2:221332/1‑251 (MQ=255)                                                                                                                                                                                          cgcgATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCAcc                                     <  2:190444/251‑1 (MQ=255)                                                                                                                                                                                                ttCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGc                               >  1:108846/1‑251 (MQ=255)                                                                                                                                                                                                ttCCCTCAGTCCGTCGCCAGTCTTACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTg                                <  2:172472/250‑1 (MQ=255)                                                                                                                                                                                                              cGCCAGACTTACCGGCGCAGATTCCTTGACAAGGGGAGCTGCAGCGGCAGTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCCCCGCTTGCCTGGCAGTTCACAt                 <  2:200253/251‑1 (MQ=255)                                                                                                                                                                                                                       tACCGGCGCATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTg        >  2:29414/1‑251 (MQ=255)                                                                                                                                                                                                                             cgcATATTCCTTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGTCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGTGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCa  <  1:147412/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGTAGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCGCCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTCTTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCATATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCTTTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCTCTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGCGCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTTGGGATCA  >  NC_000913/1875869‑1876339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑