Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,057	C→T	G308D (GGC→GAC)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,057	0	C	T	100.0%	54.3 / NA	18	G308D (GGC→GAC)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

AGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTC  >  NC_000913/1852826‑1853301                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     aGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCAGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATCACCGACGCGGAAAATGATCAATAATTAATGCCGCAATAAATATTCATACAGGAGCGTCAATCATAATAACAGCGGt                                                                                                                                                                                                                                   >  2:46569/1‑251 (MQ=255)     gTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCAtt                                                                                                                                                                                                                               <  2:55211/251‑1 (MQ=255)                                          cgACGCGAAGAAAAAATACATGTATAAAAAGAAGCTCATCACCAGTCCATAGATTAAAATCGCCCAATCTGTAGTCTGACTTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACa                                                                                                                                                                                           >  2:49172/1‑250 (MQ=255)                                                          tacatGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATcc                                                                                                                                                                           >  1:75150/1‑250 (MQ=255)                                                                      aagaagATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAGCAAATATTGTCGGTATCCATACGGTGATGGt                                                                                                                                                              <  2:23612/251‑1 (MQ=255)                                                                            atcatcACCAGTACATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATAAAGCCTAACACGGCAATAAGAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGAGCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTTTCCATACGGTTATGGTTTAAAg                                                                                                                                                        <  2:39119/251‑1 (MQ=255)                                                                                        ccATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAACTGTTCAt                                                                                                                                            >  1:241939/1‑251 (MQ=255)                                                                                        ccATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTc                                                                                                                                              >  2:6691/1‑249 (MQ=255)                                                                                        ccATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTc                                                                                                                                              <  1:6691/249‑1 (MQ=255)                                                                                         cATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCAtt                                                                                                                                           <  1:266974/251‑1 (MQ=255)                                                                                                                                  atatAGCCTAAAACGGCAAGAATAATAAGTAAGGGGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCTAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACAt                                                                                                  <  2:261832/251‑1 (MQ=255)                                                                                                                                           aaCACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAAGTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGCCAGTAATATTGATTTATCGACATCAATGCCGGAATTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTACAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCttt                                                                                         <  2:161839/251‑1 (MQ=255)                                                                                                                                            acacGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGGAATCGCGACTAATGTACGTCGTAACATTTCACCTTTa                                                                                        <  1:293404/251‑1 (MQ=255)                                                                                                                                                                             aTAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACttt                                                        >  2:95510/1‑250 (MQ=255)                                                                                                                                                                               aaCCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAAc                                                     <  2:158800/251‑1 (MQ=255)                                                                                                                                                                                  cgacgaGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTaaaa                                                                                                                                 >  1:78416/1‑172 (MQ=255)                                                                                                                                                                                  cgacgaGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGTCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTaaaa                                                                                                                                 <  2:78416/172‑1 (MQ=255)                                                                                                                                                                                                                                  ggAGCGTCAATCATAATAACAGCGGTCCTTAATATTGCTTAATCGACATCAATCCAGGACTTAACAACTATGCTCGATATCCATACGGTGATGGTATAAAGAGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGTAGTAAGCGGGGGTAAACGACTACTCTtctc  >  2:156319/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     AGGCGTAAATGCGTTGGCCAAAGCTCCGGGATATAAACCGCCGACGCGAAGCAAACATACATGTATAAAAAGAAGATCATCACCAGTCCATAGATTAAAATCGCCCACTCTGTAGTCTGAATTGAATAGATATAGCCTAACACGGCAATAATAATAAGTAAGGTGGAGCCAAATAACCGACGAGGAAAATGATCAATAATTAATGCCGCAATAAATATTCCTACCGGAGCGCCAATCATAATAACAGCGGTCATTAATATTGATTTATCGACATCAATGCCGGAGTTAACAAATATGGTCGGTATCCATACGGTGATGGTATAAAGCGAAATGTTCATTGCAATTAAAACAGTAATCGCGACTAATGTACGTCGTAACATTTCACCTTTAAACAGGAGCCAGAAAGTACCCTTGATTACTTTAACCTTGCTGTTGCTCTGATACGAAGTAAGCGGGGGTAAACGAATACTCTTCTC  >  NC_000913/1852826‑1853301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑