Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,174,359	IS5 (+) +4 bp	coding (205‑208/285 nt)	gatB ←	galactitol‑specific PTS enzyme IIB component

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2101749 =	NA (NA)	10 (0.350)	9/484	1.4	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2174359 =	0 (0.000)					coding (208/285 nt)	gatB	galactitol‑specific PTS enzyme IIB component
*	?	NC_000913	= 2174362	0 (0.000)	25 (0.860)	21/484	0.4	100%	coding (205/285 nt)	gatB	galactitol‑specific PTS enzyme IIB component
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

GGCCAATGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTATTGTTACAGAGGGATTTATTCGTCTG  >  NC_000913/2174125‑2174607                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      aaacgataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCa                                                                                                                                                                                                                                          <  1:165853‑M2/17‑1 (MQ=255)      ataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACt                                                                                                                                                                                                                                     <  1:233052‑M2/22‑1 (MQ=255)         accaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACg                                                                                                                                                                                                                                  <  1:187035‑M2/25‑1 (MQ=255)          caacaaAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATGTAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctg                                                                                                                                                                                                                                 >  1:177554‑M1/1‑229 (MQ=255)            acaaAGCCAATCCCGATATGCAGACCCGCTATAAATCAATCTCCTGCCTTCATGCCTAATATTTTAGAAAAAATAAGGATTACAATCGGCAGCATCGCCGTAAGGCCGAGCTCGAGAATATAACGCATGACTTCTGAAAACATAGGACACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATAACATAAAGAAAAGGCATTCCGTGAACTAAggaaggtgcgaacaagtccctgat                                                                                                                                                                                                                               <  2:261666‑M1/251‑25 (MQ=255)                        ccGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgt                                                                                                                                                                                                                   >  1:56825‑M1/1‑215 (MQ=255)                                 agaccatctttaaagCAATCGCCGGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGGAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGACAACATAGGTAACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatct                                                                                                                                                                                                          >  2:110816‑M1/8‑206 (MQ=255)                                     ccGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                      <  1:62887‑M1/251‑50 (MQ=255)                                      cGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagc                                                                                                                                                                                                     >  1:239511‑M1/1‑201 (MQ=255)                                         tttAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcgat                                                                                                                                                                                                  >  2:73074‑M1/1‑198 (MQ=255)                                         tttAAAGCAATCGCCTGCCTTCATGCCTAATAGTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                      <  1:73074‑M1/247‑50 (MQ=255)                                                 ggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagttagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACAccatcc                                                                                                                                                                                          <  1:237880‑M2/65‑1 (MQ=255)                                                     gcctgcctTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttc                                                                                                                                                                                      >  1:136836‑M1/1‑186 (MQ=255)                                                                  ccTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatc                                                                                                                                                                         <  2:56825‑M1/251‑79 (MQ=255)                                                                      gtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTa                                                                                                                                                                     <  2:81364‑M2/86‑1 (MQ=255)                                                                          tttAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacc                                                                                                                                                                 >  1:83285‑M1/1‑165 (MQ=255)                                                                                aaaaaGAATGATGACAATCGGCAGCAGAACCGGAGGGCCGAGGTCGAGAATATAACGCAGGACTGCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAAGTTTATTTTGTAAGGCTGCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtgtggcatctggagccatagaacaggggtcatcatgagtcatcaacttacctgcgcc                                                                                                                                                           <  2:89477‑M1/251‑93 (MQ=255)                                                                                         gatgaCAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtga                                                                                                                                                   >  2:129649‑M1/1‑150 (MQ=255)                                                                                          atgaCAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaat                                                                                                                                                 >  1:103867‑M1/1‑149 (MQ=255)                                                                                                           taaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGa                                                                                                                                 <  1:99181‑M2/122‑1 (MQ=255)                                                                                                                                aaTATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttc                                                                                                           <  1:68867‑M1/251‑141 (MQ=255)                                                                                                                                     aaCGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtc                                                                                                      >  2:15804‑M1/1‑106 (MQ=255)                                                                                                                                               ttCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatgg                                                                                              >  2:260213‑M1/1‑96 (MQ=255)                                                                                                                                               aggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCg                                                                                            <  1:59019‑M2/159‑1 (MQ=255)                                                                                                                                                           gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCa                                                                                 >  1:231341‑M2/81‑250 (MQ=255)                                                                                                                                                              aaaaaaacgtctcaaaaataacgaaataaaatgactgagtcatccgagaagaatttccccgcttattcacaccttccCTACCGGAATAACGCCAAAACTACTATCCACTTTTGCAGTGGTGCATATCAAATGCACGCCATCCATATAGGTTTCTACTTCATTAACCCGACACTTGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCTCCATCGgagaggtcttacccg                                                                             <  2:128213‑M2/174‑8 (MQ=255)                                                                                                                                                               cACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggc                                                                            >  1:128350‑M1/1‑80 (MQ=255)                                                                                                                                                                         aGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggt                                                                  <  1:93182‑M1/251‑182 (MQ=255)                                                                                                                                                                         aGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggt                                                                  <  2:239511‑M1/251‑182 (MQ=255)                                                                                                                                                                          caaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAg                                                                 <  1:249983‑M2/186‑1 (MQ=255)                                                                                                                                                                                                     ttCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttacccca                                        >  2:129983‑M1/1‑42 (MQ=255)                                                                                                                                                                                                                       aaTAAAAGGCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatggttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcga                    <  2:177554‑M1/251‑228 (MQ=255)                                                                                                                                                                                                                               gCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatcc            <  1:32751‑M1/251‑236 (MQ=255)                                                                                                                                                                                                                               gCATGCCGTGAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatcc            <  1:91733‑M1/251‑236 (MQ=255)                                                                                                                                                                                                                                         aaCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaacc  >  1:104011‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      GGCCAATGCCAACAAAGCCAATCCCGATATGCAGACCCGCTTTAAAGCAATCGCCTGCCTTCATGCCTAATATTTTAGAAAAAATAATGATGACAATCGGCAGCATCACCGTAGGGCCGAGGTCGAGAATATAACGCATGACTTCTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCCGCAAGCGACAATAATCTTGCGTTTCATAAATAGTCCTTTTTATTGTTACAGAGGGATTTATTCGTCTG  >  NC_000913/2174125‑2174607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.