Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,938,441	Δ1 bp	coding (215/993 nt)	rbsR →	DNA‑binding transcriptional dual regulator RbsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,938,441	0	C	.	100.0%	120.7 / NA	27	coding (215/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (10/17);  total (10/17)

ACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGC  >  NC_000913/3938198‑3938682                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   aCGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTAtt                                                                                                                                                                                                                                            <  2:218678/251‑1 (MQ=255)           aGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGt                                                                                                                                                                                                                                  >  2:94597/1‑251 (MQ=255)                ggCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTg                                                                                                                                                                                                                             <  2:253374/251‑1 (MQ=255)                        tGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGa                                                                                                                                                                                                                      >  2:275063/1‑250 (MQ=255)                                               ggTTGCCCGCCTGGAGGGCGTTTCTACCTAAACAGTGTCTCACGTTATCAATAAAGATCGCGTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATGAAAGAACACAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAAGCAAACACATACCATGGGCATGGTGATCACTGCCAGTACCAATC‑TGTCTATTCAGAACTGGGGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTta                                                                                                                                                                                              <  2:143953/250‑1 (MQ=255)                                                ggtGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTtat                                                                                                                                                                                             <  1:83473/249‑1 (MQ=255)                                                                                   ttCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAAc                                                                                                                                                           <  1:303845/250‑1 (MQ=255)                                                                                    tctcACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACa                                                                                                                                                          <  2:280541/250‑1 (MQ=255)                                                                                        aCGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACTATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCgg                                                                                                                                                      >  2:212346/1‑250 (MQ=255)                                                                                          gTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATg                                                                                                                                                   >  1:50511/1‑251 (MQ=255)                                                                                                                         cGATTACCGCCAAAGGTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTTAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAAc                                                                                                                    <  2:8755/251‑1 (MQ=255)                                                                                                                           aTTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAAcgc                                                                                                                  >  1:63432/1‑251 (MQ=255)                                                                                                                              aCCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGtt                                                                                                               <  1:183699/251‑1 (MQ=255)                                                                                                                                             gcggcgATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTa                                                                                                <  1:129339/251‑1 (MQ=255)                                                                                                                                                      aaaGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CAcc                                                                                      <  1:238164/251‑1 (MQ=255)                                                                                                                                                             tCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGc                                                                               >  2:78001/1‑251 (MQ=255)                                                                                                                                                               aaTTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCAt                                                                             <  2:16688/251‑1 (MQ=255)                                                                                                                                                                      cgcCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGCTGCGTGACGTTGAACGCCGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGCAGGCGATGAACAGCGGCTGACTCGCAATCTGGCAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCtt                                                                      >  2:160476/1‑251 (MQ=255)                                                                                                                                                                      cgcCATCAGCGCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCAGTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCtt                                                                      <  2:305922/251‑1 (MQ=255)                                                                                                                                                                                  tGGCGCGTAGCATCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATc                                                           >  2:250712/1‑250 (MQ=255)                                                                                                                                                                                          cgCCTCAAACTCAATCAAACACATACCATTGTCTTGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGt                                                  <  2:227192/250‑1 (MQ=255)                                                                                                                                                                                            ccTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTa                                                <  1:228246/251‑1 (MQ=255)                                                                                                                                                                                                                  aCCATTGGCATGTTGATCACTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGtgatg                          >  1:53822/1‑251 (MQ=255)                                                                                                                                                                                                                                    gcTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTGGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTc        <  2:265889/250‑1 (MQ=255)                                                                                                                                                                                                                                    aCTGCCAGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTc        >  1:178738/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCTGCCAGTACCAATC‑TTTCTATTCAGAACGGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTGGCAATACCGAATGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAATGGTGTTGATGGCTTGCGGTTGC‑GTGGCGCCGGAACGGATCAACCTTCTCGGGAAATCATGCAACGTGTGCGGACGGTGTCTACTGTGATGTTGGATTGGGGTGCGTTc        <  2:151545/251‑1 (MQ=255)                                                                                                                                                                                                                                          aGTACCAATC‑TTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCATCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGc  <  2:221823/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ACGCATTTTTAGACAGGCAGAGGTGACGCTTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTTCGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCCTCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGCGTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAATCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTG‑CACCGAAACGCATCAACCTTCGCGTGAAATCATGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGC  >  NC_000913/3938198‑3938682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑