Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	190.0 / NA	41	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/21);  total (20/21)

TGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCAC  >  NC_000913/3207319‑3207796                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        tgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaa                                                                                                                                                                                                                                          >  1:152315/1‑245 (MQ=255) tgGTTCATGCCGATCTAACCTTTGAAAATCTGTCTCACCCCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGGTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaa                                                                                                                                                                                                                                          <  2:152315/245‑1 (MQ=255)  ggTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGc                                                                                                                                                                                                                                   <  1:295644/251‑1 (MQ=255)            gATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATggg                                                                                                                                                                                                                         >  2:222199/1‑251 (MQ=255)                tcACCTTTGAAAATCTGTCTCACAACCGGAGCGCGTTATGAAACAGATCCTG‑ACAACCCCGATCAAAGCTGAAGCTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATggg                                                                                                                                                                                                                         >  1:190351/1‑247 (MQ=255)                 cACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAt                                                                                                                                                                                                                     <  1:260382/250‑1 (MQ=255)                      ttGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtct                                                                                                                                                                                                                <  1:86846/250‑1 (MQ=255)                      ttGAAAATCTGTCCCCCACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGCCCAGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtc                                                                                                                                                                                                                 <  2:214045/249‑1 (MQ=255)                        gaaaaccggccccacccccGGCGCGCGGTAGGAAAAAGA‑CCTGGACAACCCGGAGCAAAGCTAAAGATCTGAAAGATATTCGCGTCGGCGATGTGATCGCCCTGACCGGTACGCTGGTGCCCTGCCGCGGCGTTTGTCACCGCCGTTTGATCGAACCGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCg                                                                                                                                                                                                             <  2:28944/235‑1 (MQ=255)                                 tctcACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCATGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCgc                                                                                                                                                                                                    >  2:185353/1‑250 (MQ=255)                                           ggAGCGCTTTATGAAAAAGACCCTG‑ACACCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCTTCGGCGATGTGATCTCCCTGACCGGTACTCTGGTGACCTGCCGCGCCGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGTTAGATGGGCTCCGTCGGCCCGACATCCAGTAt                                                                                                                                                                                          <  1:115018/251‑1 (MQ=255)                                              gcgcGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCg                                                                                                                                                                                       <  1:249171/251‑1 (MQ=255)                                                                     aCAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCCAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGAAAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACggga                                                                                                                                                                  >  2:234617/1‑247 (MQ=255)                                                                          cccGAACAAAGCTGAAGATCTGCAAGATATTCGCGGCGGCGATGTTATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTAACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAt                                                                                                                                                            <  2:136389/251‑1 (MQ=255)                                                                              aTCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGAGACCTGACGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTg                                                                                                                                                          >  1:189207/1‑249 (MQ=255)                                                                               tCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                       >  2:167631/1‑251 (MQ=255)                                                                                      tGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCgg                                                                                                                                                >  1:248258/1‑251 (MQ=255)                                                                                      tGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCgg                                                                                                                                                >  1:259673/1‑251 (MQ=255)                                                                                            tCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGa                                                                                                                                           >  1:237424/1‑250 (MQ=255)                                                                                            tCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGa                                                                                                                                           >  2:96555/1‑250 (MQ=255)                                                                                            tCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGa                                                                                                                                           >  2:146650/1‑250 (MQ=255)                                                                                                          gcgTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGc                                                                                                                             >  2:110583/1‑250 (MQ=255)                                                                                                                     gtggcttgccttaCCGGGACGTTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACTGCGACAAATGGGAGATGGGCGCCGTCGTCCCGACATCCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATg                                                                                                                 <  1:153746/239‑1 (MQ=255)                                                                                                                          cTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGcc                                                                                                            >  2:27885/1‑251 (MQ=255)                                                                                                                            aCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGc                                                                                                          >  1:16061/1‑251 (MQ=255)                                                                                                                              cggACCGGTCCGCTGGGGCCCTGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTg                                                                                                        <  2:278424/249‑1 (MQ=255)                                                                                                                               tGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTg                                                                                                        >  1:254111/1‑250 (MQ=255)                                                                                                                                    ggTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaa                                                                                                  <  1:199524/251‑1 (MQ=255)                                                                                                                                     gtacctggtggccctcccGCGACTTTTGCCACCGCCGTTTGATCGAACGGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCCCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTATCCGTCGGGCCGACAACCAGTATGCGTATGGAAAGTTTTGAGCGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaag                                                                                                 <  2:162675/235‑1 (MQ=255)                                                                                                                                     gTACGCTGGTGCCCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaag                                                                                                 <  1:76915/251‑1 (MQ=255)                                                                                                                                     atACGCTGGTGACCTGCCGCGACGTTTGTCCCCGCCGTTTGATCGAACTGAAACCTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAAAGGGCGATGGACTCCGTCGGCCCGACAACCAGTATGCGAATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTGGGCTAAGGTGGTAGGGGTCCGCTGACCga                                                                                                   >  2:40147/2‑249 (MQ=255)                                                                                                                                         gCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAgg                                                                                             >  2:76562/1‑251 (MQ=255)                                                                                                                                             gggcccggCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGc                                                                                         <  2:259673/244‑1 (MQ=255)                                                                                                                                                    gCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCGGACAACCAGTATGCGTATGGAAAGTTTCGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGaa                                                                                    <  1:186988/249‑1 (MQ=255)                                                                                                                                                                gTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCtcc                                                                      >  1:157635/1‑249 (MQ=255)                                                                                                                                                                   cccgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtg                                                                   <  1:17777/250‑1 (MQ=255)                                                                                                                                                                        cgTTTGATCGAACTGTAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGTCCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAtt                                                              <  2:199174/251‑1 (MQ=255)                                                                                                                                                                            tGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTc                                                           <  1:136325/250‑1 (MQ=255)                                                                                                                                                                                cGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATTGGGCCGCTGACCGAAGAAGGCTGCCAGAAAGTCAATGCGCt                                                                        <  1:57989/233‑1 (MQ=255)                                                                                                                                                                                cGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCt                                                                        >  2:57989/1‑233 (MQ=255)                                                                                                                                                                                  aaCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAg                                                    <  1:146650/251‑1 (MQ=255)                                                                                                                                                                                                                                     gctgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCAc  <  1:185353/248‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                        TGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTG‑ACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCAC  >  NC_000913/3207319‑3207796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑