Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336,901	0	T	A	33.3%	33.7 / 11.0	30	Y44F (TAT→TTT)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (17/3);  new base A (0/10);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 9.52e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTAT  >  NC_000913/4336654‑4337139                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               ggTTAATACCAATGGATCTTTCAGAATCGTGAAGAACTAACTTAAATATCCTACGCCAATGACCACCATGGCTATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGTGCCAAAGCAGCTGCGGTCGTAAGCGTAAGAACCTCCAGGTCTTTTGTCTAGGAACGACATTTTGTCGTATACCATCTAGAGCCCCAGTGCACCGATAATCTTCACCAACG‑ATCCATaa                                                                                                                                                                                                                                              <  1:73179/250‑1 (MQ=255)              ggATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCCAGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGGAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAAAGACATTTTGGCGTATACCATCGAGAGCCCCCGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGcc                                                                                                                                                                                                                                >  2:179553/1‑251 (MQ=255)                    ttCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGATTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTTGGTCGAGGAACGAACTTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGtcg                                                                                                                                                                                                                           >  1:171998/1‑248 (MQ=255)                                    agTAACTTAAATATCATACGCCAATGATCACCATGGCGATATTGCCGTTCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCACAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCCAGAGCACCAGTGCACCGATAATCGTCACCACCC‑ATCCATAAACGGCAATACCGCCAGTAGAGGCCAGGTTTGccggg                                                                                                                                                                                                          >  2:210052/1‑247 (MQ=255)                                         cTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCCGGTTTGCAGGTAACa                                                                                                                                                                                                      >  2:5296/1‑250 (MQ=255)                                                        ccaaggcccaccgTGGGGATATTGCCGACCCGGCAGGCAAGCCAGAAGGGGACGTTGGTTGGAAAACCAAAAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGGCGAGGAACGAATTTTTGGCGTATACCATCGAGGGCCCCAGTGCCCCGATAACCGTCACCACCC‑ACCAAAAAATGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAccc                                                                                                                                                                                      <  1:46140/244‑1 (MQ=255)                                                              ccccccaTGGCGATATTGCCGCCCCAGCAGGCCAGCCAGGAGAGGACGTTGGTTTGATAACCGAGAAACGGGCAAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGACCCCCAGTGCCCCGATAATCGTCACCAACC‑ACCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCataat                                                                                                                                                                                <  1:104854/247‑1 (MQ=255)                                                                          atatTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAAATGTCACCAACC‑ATCCCTAAATGGCAATCCCGCCAGTTGAGGCCCGGGTTGCAGGTACCAGAAAAACACCTGACCCCCTACTATTcccatcaac                                                                                                                                                                    >  2:52038/1‑245 (MQ=255)                                                                              tGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATc                                                                                                                                                                >  2:107734/1‑251 (MQ=255)                                                                                  gATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAggg                                                                                                                                                            >  1:18112/1‑251 (MQ=255)                                                                                   aTCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATCACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGCCTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGCGAGACCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATACCCCCAGTAGAGGCCAGGTTTGCCGGGAACAGAAAAACAACTGACCCCCTAATATTCCCCGACACCAtacgggg                                                                                                                                                           >  2:24399/1‑245 (MQ=255)                                                                                    tCCAGCAGGCCAGCCAGTAGAGCACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGGAAGCGTAAGAAACACCAGGACTTGGGTCGAGGAACGAAATTTTGGCCTAAACCATCGAGAGCCCCAGGGGACCGATAATCGTCACCAACC‑ATCCATAAAAGGCAATACCCCCAGCAGAGGCGAGGTTTGCAGGGACCAGAAAAACACCTGTCCCCatcatcatccccaccgcgataacattg                                                                                                                                                          >  2:233062/1‑226 (MQ=255)                                                                                                        gggtggttggttgGAAAACCGAAAAACGGGCAAAAGCAGGGGCGGGCGTAAGCGAAAGAACCCCCAGGACTGGGGGCGAGAAACGACATTTGGGCTTATACCACCGAGAGCCCCGGTGCCCCGATAAGCGTCACCAACC‑ACCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACttt                                                                                                                                      <  1:181506/246‑1 (MQ=255)                                                                                                         ggACGTGGGTTTGATAACCGAGAACCGGGCAAAAGCGGCGGCGGGCGTAAGCGAAAGAACCCCAAGGATTTGGGTCGAGGAACGACTTTTTGGCGTATCCCATCGAGACCCCCAGTGCACCGATAATCGCCCCCAACC‑ACCAAAAAATGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtg                                                                                                                                     <  1:107734/251‑1 (MQ=255)                                                                                                                     gaaacacGAGAAGCGGGCAAAAGCAGGGGGGGACGTAAGCGAAAGAACCACCGGGACTGGGGTCGAGGACCGACATTTTGGAGTATACCAGGGGGACCCCCAGGGCCCAAATAACCGTC‑CCAACCCACCCAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                          <  2:276772/244‑1 (MQ=255)                                                                                                                     gATAACCGAGAAGCGGGCCAAAGCAGGGGGGGGCGAAAGCGAAAGACCCACCAGGACTTGGGTCGAGGACCGACATTTTGGCGTATACCACCGAGAGCCCCAGGCCACCGATAACCGTCACCACCC‑ATCCAAAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                                          <  2:2161/250‑1 (MQ=255)                                                                                                                       tAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagcatc                                                                                                                       >  1:322331/1‑251 (MQ=255)                                                                                                                          ccGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCg                                                                                                                     >  2:141122/1‑250 (MQ=255)                                                                                                                                aaggggcaaaaggggcggggGGCGTAAGCGTAAGAACCACCAGGACTGGGGTCGAGGAACGACATTTGGGCGTATCCCATCGAGACCCCCAGTGCCCCGATAATCGTCACCAACC‑ACCCAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACAt                                                                                                              <  1:363932/237‑1 (MQ=255)                                                                                                                                      caaaaGCAGCGGCGGGCGTAAGCGTAAGAACCACAAGAACTGGGGTCGAGGAACGACATTTGGGCGTATCCCATCGAGACCCCCATTGCACCGATAATCGAC‑CCAACCCACCCATAAATGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTaa                                                                                                        <  1:10499/249‑1 (MQ=255)                                                                                                                                               cggcggGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCg                                                                                               >  2:134548/1‑251 (MQ=255)                                                                                                                                                                        aaggacgggggTCGGGGAACAACATTTGGGCGTATCCCATCAAGACCCCCAGTGACCCGATAAGCGCCCCCAACC‑ATCAAAAAAGGGAAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAGTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATGAAACTCCTGCGAAGGCGAGCGTCGTGACAATAAAtt                                                                      <  2:189175/243‑1 (MQ=255)                                                                                                                                                                         aGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAtt                                                                      >  2:264739/1‑250 (MQ=255)                                                                                                                                                                                       gaaaCGACATTTGGGCGTATCCCACCGAGACCCCCAGTGCCCCGATAATGTTCCCCACAC‑ATCCAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACg                                                       <  1:69089/249‑1 (MQ=255)                                                                                                                                                                                         aaCGACATTTTGGCGTATCCCATCGAGACCCCCAGGCCCCCGATAACCGCCACCAACC‑ACCAAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                                     <  1:141122/251‑1 (MQ=255)                                                                                                                                                                                                            ccATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                                  >  1:300645/1‑251 (MQ=255)                                                                                                                                                                                                                   gagCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCg                           >  2:199157/1‑251 (MQ=255)                                                                                                                                                                                                                           gTGCACCGATAATCTCCACCAACT‑ATCAAAAAAGGCCATTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGtt                   <  1:109787/251‑1 (MQ=255)                                                                                                                                                                                                                                 cGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCt             >  1:241883/1‑251 (MQ=255)                                                                                                                                                                                                                                            caccaaatatccatAAATGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCACCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGAGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATCGTTGAATTATTAGCGACCGGGTTATAGCTGATTCCGttc   >  2:97958/9‑250 (MQ=255)                                                                                                                                                                                                                                            accaacc‑aTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGttat  >  2:77850/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GGTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACC‑ATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGATGCCGTTAT  >  NC_000913/4336654‑4337139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑