Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,027,2330TA51.8% 1.2 / 25.1 27intergenic (+5/+376)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (9/4);  new base A (0/14);  total (9/18)
Fisher's exact test for biased strand distribution p-value = 1.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTC  >  NC_000913/4027009‑4027463
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      
ttggCAAGTGGGGCCGGTGGTTGAACAACCGGAGGCGGGCTGGCGTGGGCGTACTGGCCCCGTTTTAACGGGGTTATTGCAGGATCACGGTACGCTGGCAGAGCAGGATATCTATATGGCCGGACTTTTGG‑AGAGGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCTTGTGG‑GGCGATGTGTTGG‑CATTTATCTGAAATATAAAAAAACCCGCCCCTGACAGGCgg                                                                                                                                                                                                               <  1:272779/247‑1 (MQ=255)
              cGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTTCAGGGTCCCCGTACGCTGGGCGAGCATGATATTTATATTTCCCGGCGTTTTT‑AGGTGGCGAAAATTGACCCCGGTCTTTTTTTTCGTGAGGGTTAAT‑CGCGGGGAGAT‑CTCCTGTTT‑TGCGATGGGTTTT‑CATTTTTTTTCGATTTAAAAAAACCCCCCCCTgcccaggggggggaaacggcaca                                                                                                                                                                                                 >  2:297605/1‑228 (MQ=255)
                gtggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTTCCGGATCCCGGGACGCTGGCCGAGCCTGATATCTATATTTCCGGACGTTTTT‑AGGTGGGGAAAAATGCCCGCGATCTGTTTTTCAGTGGGCGT‑AATGCGCGGGGAGAT‑CGCCCGTTT‑GGGGATGCGTTTG‑CCTTTTTCTTAGGTATAAAAAAACCCGCCCCCGACAGGCGGGGAGAAAGGCaaaaaa                                                                                                                                                                                               >  2:51280/1‑246 (MQ=255)
                ggggtTGAACAACCGGAGGCGGGCTGGCGTGGGCGTACTGGCCCCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTG‑AGATGGCAAAAATTGCCCGCGATCTGTTTGGCAGTGGGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTG‑GGCGATGCGTTTG‑CATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCaa                                                                                                                                                                                                   <  1:51280/244‑1 (MQ=255)
                         caacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGGTCACGGGAAGCTGGCAGAGCATGATATCTATATTTCCGGGCGGTTTG‑AGATGGGGAAAAATGCCCGCGATCTGTTTTGCCGTGAGCGG‑AATGCGCGGGGAGAT‑CGCCTGTTT‑TGGGATGCGGTTG‑CATTTTTCTGAGATATAAAAAAAACCGCCCCTGACAGGCggggaaaaagggaaaaaaacagttattt                                                                                                                                                                                     >  1:249877/1‑226 (MQ=255)
                          aacgggaggggggggggggTGGGCGTACGGGCACCGTGTTAACGGCGGTATTGCAGGAGCACGGTACGCGGGCAGGGCATGATATCTATATTGCGGGACGTTTGG‑AGAGGGGGAAAATTGCCCGCGATCGGTTTTGCGGGGGGGGT‑AAGGCGCGGGAAGAT‑CGCCTGTTG‑GGCGATGCGTTTG‑CATTTATCTGAAAAAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCa                                                                                                                                                                                    <  1:193265/233‑1 (MQ=255)
                                              gggCTTACTGGCACCGTTTTAACGGCGGTTTTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACTTTTGG‑AGATGGCGAAAATTGCCCGCGATCTTTTTTGCAGGGAGCTT‑AATGCGCGGGAAGAT‑CGCTTGTTT‑GGCGATGCGTTTG‑TATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGAc                                                                                                                                                                <  1:290008/251‑1 (MQ=255)
                                              gggCGTACTGGCCCGGTGTTAACGGCGGTATTGCAGGATCACGGTAGGCTGGCAGAGGATAATATCTATATTGCCGGACGTTTGG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGGGT‑AATGGGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGa                                                                                                                                                                 <  1:200572/250‑1 (MQ=255)
                                               ggCGTAGTGGCACCGTGTTAACGGCGGTATTGCAGGATCGAGGTACGGTGGCAGAGCATGATATCTATATTGCCGGACGTTTTG‑AGAGGGAGAAAATCGCCCGCGTTCTGTTTTGCAGTGAGCGT‑AAGGGGCGGAAAGAC‑CGCCTTTTT‑GGCGATGGGTTGG‑AATTTATCTGAAAAAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACg                                                                                                                                                               <  2:152684/251‑1 (MQ=255)
                                                             gggtaaagggcgtTATTGCAGGAACAGGGTACGTGGGCAGAGCATGATATCTATATTGCCGGCCGTTTGG‑AGAGGGAGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGGGGGAAGAT‑CGCCGTTTT‑GGCGAGGCGTTTG‑CATTTATCGGAGAAAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCa                                                                                                                                                 <  1:117949/243‑1 (MQ=255)
                                                                     gggggtatgtcAGGACCACGGTAGGCTGGCAGAGCGTGATATCTATATTGCCGGCCTTTTGG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGAAAGAT‑CGCTTGTTT‑GGCGAGGGGTTTG‑CATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGAt                                                                                                                                         <  2:112606/241‑1 (MQ=255)
                                                                               cAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGt                                                                                                                               >  1:100501/1‑251 (MQ=255)
                                                                                         cgagcctctcccgttctGATATTTATTTTGCCGGACGTTTTG‑AGGTGGCAAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGAAAGAC‑CGC‑TGTTTTGGCGAGGCGTTTG‑CTTTTATCTGAGATTAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTAt                                                                                                                     <  2:97251/235‑1 (MQ=255)
                                                                                                      gattGATATTTATTTTGCCGGA‑GTTTTGGAGATGGACAAAATTGCCCGCGTTCTGTTTTGGAGTGGGGGT‑AAGGGGCGGGAAAAT‑CGCCTGTTT‑GGCGATGCGTTTG‑AATTTATCGGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGGGCCTACAAAt                                                                                                        <  2:298926/248‑1 (MQ=255)
                                                                                                          gATATCTATATTGCCGGACGTTTTG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGGAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATTGTGCCTACAAATAGTc                                                                                                    >  2:148844/1‑251 (MQ=255)
                                                                                                          gATATCTATATTGCCGGACGTTTTG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTAt                                                                                                                     >  1:97251/1‑234 (MQ=255)
                                                                                                            tatatttatggcgggACGTTTTG‑AGAGGGAGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGGGGGAAGAC‑CGCCTTTTT‑GGCGAGGGGTTTG‑CATTTATCTGAAATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc                                                                                                   <  2:107712/237‑1 (MQ=255)
                                                                                                                 atatTGCCGGACTTTTGG‑AGATGGCAAAAATTCCCCGCGATCTTTTTTGGAGGGAGCGA‑AGGGCGAGGGAA‑ATGCGCTTTTTG‑GGCGTGGTTTTTG‑CATTTATCTGATATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAcc                                                                                              <  2:336408/250‑1 (MQ=255)
                                                                                                                       ccGGACGTTTGG‑AGATGGCAAAAATTGCCCGCGATCTTTTTTGCAGTGAGCGT‑AATGCGGGGGAAGAT‑CGCCTTTTG‑GGCGATGCGTTTG‑CTTTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGcc                                                                                       <  1:309186/251‑1 (MQ=255)
                                                                                                                                         gCGAAAATTGACCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAATGGCATTTAAATCTATGAAGTATCTGGCAAAACATGCCCGACGCAACGCTGTCCCGTCTTATCGTGACTACAAAAAGTCCGAACCGGAAGCCggcaaagggctttacgc                                                                      >  2:158146/1‑236 (MQ=255)
                                                                                                                                                  gCCCGCGTTCTGTTTTGCAGTGAGCGT‑AAGGCGCGGAAAGAT‑CGCCTGTTG‑GGCGATGGGTTTG‑CATTTATCTGAGATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                              <  1:250683/250‑1 (MQ=38)
                                                                                                                                                  ccccggattttGTTTTGCAGTGGTCGA‑AAGGCGGGGGAAGAT‑CGCTTGTTG‑GGCGTTG‑GTTTGGCATTTATCGGAGATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                             <  2:326049/241‑1 (MQ=25)
                                                                                                                                                                                          gAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCt                      >  1:9359/1‑251 (MQ=255)
                                                                                                                                                                                              cGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGGAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCTTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCttct                   >  2:76770/1‑249 (MQ=255)
                                                                                                                                                                                              cGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTa                    <  1:113368/250‑1 (MQ=255)
                                                                                                                                                                                                              gCGTTTG‑CATTTATCTGAAATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAg    <  1:278222/251‑1 (MQ=255)
                                                                                                                                                                                                                gTTTG‑AATTTATCTGAAATATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAGATAGTc  <  1:297605/251‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      
TCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTG‑AGATGGCGAAAATTGCCCGCGATCTGTTTTGCAGTGAGCGT‑AATGCGCGGGAAGAT‑CGCCTGTTT‑GGCGATGCGTTTG‑CATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTC  >  NC_000913/4027009‑4027463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑