Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,203,311	0	T	A	32.0%	92.7 / 17.3	51	*274L (TAA→TTA)	bacA	undecaprenyl pyrophosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (23/11);  new base A (0/16);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 2.70e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGACCCGACGGCGGATTGCGGCGGTAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGC  >  NC_000913/3203065‑3203561                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                           tGACCCGACGGCGGATTGCGGCGGTAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTaaa                                                                                                                                                                                                                                                         >  1:16111/1‑250 (MQ=255)  gACCCGACGGCGGATTGCGGCGGTAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAg                                                                                                                                                                                                                                                       <  1:113241/251‑1 (MQ=255)      cGACGGCGGATTGCGGCGGTAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAaca                                                                                                                                                                                                                                                   <  2:280755/251‑1 (MQ=255)          ggcggATTGCGGCGGTAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGAc                                                                                                                                                                                                                                               >  1:216002/1‑251 (MQ=255)                 tGCGGCGGTAGCCATCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAACCGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATacac                                                                                                                                                                                                                                         <  1:196914/250‑1 (MQ=255)                  gcggcgGTAGCCAGCTGGAAGGAACAGCGTTGCCCAAAGCCTGAATGAGTATTGGTCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCGGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGGGAA‑CGCGTTATTCGGCCTGCACAAGCGTGGACAACGTCAAGCAGCCGACACTATTCCTCAATGAAAAGAACACGGCATACACCg                                                                                                                                                                                                                                       <  2:21664/251‑1 (MQ=37)                         tAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAgccgc                                                                                                                                                                                                                                >  1:81923/1‑251 (MQ=255)                                   gaaggaaCAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATaa                                                                                                                                                                                                                        >  1:150960/1‑249 (MQ=255)                                           aacGGTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACAAACACCGCAGCCGCCACAATAAAGCGATaaa                                                                                                                                                                                                               >  2:92029/1‑250 (MQ=255)                                              gTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGc                                                                                                                                                                                                           >  1:238936/1‑251 (MQ=255)                                                   caaaaGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCTTAACCGCTTTACCCGGCTACATCTCAACGACATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACCCTATTCCTCAATAAAAAGAACACGACATACACCCCAGCCGCCACAATAAAGCGATAAATGGCGAACg                                                                                                                                                                                                      <  2:369782/249‑1 (MQ=38)                                                           ggaaggAGTATTGGGCATTTTGCCGGATGCGGCGAAAACGCCTTACCCGGCTACATGTCAACGACAGTTGTGGGCTTGAAAAGCCCCGCACGCGCCGAATCAGGCGTTGTTTGCCGGATGCGCGGTGAA‑CCCCTTATTCGGCCTACACAATCGTTGACAACACAAAGCACCCGACCCTATTCCTAAATAAAAAGAACACGACATGCCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAAc                                                                                                                                                                                              <  2:9312/246‑1 (MQ=255)                                                           ggAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAACCGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGC‑TGAAACGCCTTATTCGGCCTCCGCAATCGTTGACAACGCCAAGCATCCGACCCTATTCCTCAATAAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAAc                                                                                                                                                                                              <  1:164395/250‑1 (MQ=38)                                                                gattATTGGTCATTTGGCCGGATGGGGCGTAAACGCCTTATCCGTCTACATGTCAACGACATTTTTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGCCCTACGCAATCGTTGACACCCCCAAGCATCCGACACTATTCCTCAATAAAAAGAACACGCCAGACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAAt                                                                                                                                                                                         <  2:321633/248‑1 (MQ=38)                                                                gAGTTTTGGGCATTTGGCCGGATGGGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTGGTAGGCCTGAAAAGCCGCGCAAGCGTCGCATCAGGCGTTGATGGCGGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGAAAACGCCAACCATCCGACACTATTCCTAAATAAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAAt                                                                                                                                                                                         <  2:39522/251‑1 (MQ=255)                                                                       gggCATTTGGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCTTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATAAAAAGAACACGAAATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTa                                                                                                                                                                                  <  1:240820/251‑1 (MQ=255)                                                                        ggCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTa                                                                                                                                                                                  >  1:142545/1‑250 (MQ=255)                                                                                  cGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCACCGCCAGCTGTCGGACTGATAAGACGCGCACGCGTCGCATCACGCGTTGATTGCCGGATGCGGCGTGAA‑CGACTTATTCGGCCTACGCAATCGTTGACACCGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGc                                                                                                                                                                         >  1:213403/1‑249 (MQ=255)                                                                                         ggcgTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGtt                                                                                                                                                                >  1:208434/1‑251 (MQ=255)                                                                                               aacgcttgaTCCGGCTACATGGCAACGACAGTTGTAGGCCGGAGAAGACGGGCAGGCGGTGCATAGGGCTTTGATTGCAGGATGGGGCGTGAT‑TGCTTTATTCGGCCCACGTAACCGTTGACAACGCAAAGCATCCGACACTATTCCCCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGAGCTTAATCAATTGCAGGAAGGTTTTAATc                                                                                                                                                          <  2:305064/243‑1 (MQ=255)                                                                                                      tATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCGGGCGTTTATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTCCGCAATCGTTGACAACGCCAAGCATCCGACTCTATTCCAAAATAAAAAGAACACGACAACCACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTAt                                                                                                                                                     <  1:202726/249‑1 (MQ=255)                                                                                                            gCTACATGCCAACGGCATTTGTGGCCCAGATAAGAGGCGCAGGCTCCGCAGCGGGCTTTGATGGCTGTATGCGCGGTGAA‑CCCCTTATTCGGCATCCGCAATCGTTGACAACCCAAAGCATCCGCCGCTATGCCTAAATAAAAGGACCACGACATACACCGCGGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCTCTGAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGcca                                                                                                                                             <  2:252275/251‑1 (MQ=255)                                                                                                             cTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGccac                                                                                                                                            >  1:264564/1‑251 (MQ=255)                                                                                                             atacagttcaccGACAGTTGGAGGCCTAAAAAGCCGCGCAAGCGTCGCATCAGGCGTTGATTGCGGGAGGCGGCGTTAA‑CGCTTTCTTCTGCTAACTCAATCGTTGACAACGCCAAGCAGCCAACACTATTCCTCAATAAAAAGAACCCAACATACCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGccac                                                                                                                                            <  2:157779/240‑1 (MQ=255)                                                                                                                cATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCcaccac                                                                                                                                         >  1:968/1‑251 (MQ=255)                                                                                                                   gTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGCCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTCCGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTAAATTAAAAGAACACGCCATACCCCCCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACaaa                                                                                                                                      <  1:326216/251‑1 (MQ=255)                                                                                                                     cAACGACAGTTGTAGGCCGAAGAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGAGGGGGCGTAAA‑CGCTTTATTGGGCCTGCGCAATCGTGGACAACGCCAAGCATCCGACACTATTCCTAAATAAAAAAAACAGGACTTACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATGGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACaaaa                                                                                                                                     <  1:156108/250‑1 (MQ=255)                                                                                                                      aaCGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCAAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGc                                                                                                                                   <  1:90186/251‑1 (MQ=255)                                                                                                                                  gggCCTGATAAGACGCGCAAGCGTCGCATCGGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCACCCGACACTATTCCACAATAAAAAGACCACGACATACCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAAccc                                                                                                                       <  2:81923/250‑1 (MQ=255)                                                                                                                                     ccTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATCCACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAAc                                                                                                                    <  1:157089/251‑1 (MQ=255)                                                                                                                                       tGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACgg                                                                                                                  >  2:279591/1‑251 (MQ=255)                                                                                                                                        gagaaGACGCGCAAGCTCCTCATCAGCAGTTGTTGGCCGGATGCGGCTTGAA‑CCCCTTATTCGCCTTACGCAATCTTTACCAACGCCAAGCATCCGACACTTTTCCTCAATTAAAAGAACACTACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACgg                                                                                                                  <  1:269873/247‑1 (MQ=255)                                                                                                                                              aCGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAGCGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACAt                                                                                                           >  2:182964/1‑251 (MQ=255)                                                                                                                                                gcgcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAC‑CGCCTTATTCGGCCTACGCAATCGTGGACAACCCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACAGAAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCg                                                                                                         <  2:82871/251‑1 (MQ=255)                                                                                                                                                       cGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAAAATCGGGATATc                                                                                                  >  1:223972/1‑251 (MQ=255)                                                                                                                                                         tCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATcgc                                                                                                >  1:116781/1‑251 (MQ=255)                                                                                                                                                               cAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTg                                                                                          >  1:162032/1‑251 (MQ=255)                                                                                                                                                               cAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTAAATAAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCtt                                                                                           <  1:279591/250‑1 (MQ=255)                                                                                                                                                                                         gAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTag                                                                   >  1:199188/1‑248 (MQ=255)                                                                                                                                                                                         gAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTag                                                                   >  1:184848/1‑248 (MQ=255)                                                                                                                                                                                           a‑CGCCTTATTCGGCCTAAGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACAACATCCACCGCAGCCGCCACAATAAAGCGCTAAATGGCGAACGGGCTAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCCGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTag                                                                   >  2:187461/1‑246 (MQ=255)                                                                                                                                                                                              gCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCg                                                             >  2:135368/1‑250 (MQ=255)                                                                                                                                                                                               ccTTATTCGGCCTACGAAATCGTGGAAACCGCCAAGCACCCGACCCTATTCCTAAATAAAAAAAACACGACATCCCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAg                                                           <  2:216002/251‑1 (MQ=255)                                                                                                                                                                                                      cGGCCTACGCAATCGTTGAAAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGAAATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGtt                                                    <  1:159486/251‑1 (MQ=255)                                                                                                                                                                                                       gcccTACGCAATCTTTGACAACCCCAAGCACCCGACACTATTCCTAAATAAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTg                                                   <  2:72099/249‑1 (MQ=255)                                                                                                                                                                                                              gcaaacttatacaccGCCAACCATCCGAAACTATTACACAATAAAAAGAACACGACATCCACCGAAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGTATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTATAGATCGAGCGCCGTTGCGCCcat                                            <  2:366808/237‑1 (MQ=255)                                                                                                                                                                                                                aaTCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCcatc                                           >  2:67346/1‑250 (MQ=255)                                                                                                                                                                                                                       gACAACGCCAAGCACCCGACACTATTCCTAAATAAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCg                                    <  1:113947/250‑1 (MQ=255)                                                                                                                                                                                                                          aaCGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCGGCAc                                >  1:150682/1‑251 (MQ=255)                                                                                                                                                                                                                           ccGCCAAGCACCCGACACTATTCCTCAATTAAAAGAACCCGACATACCCCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCGGCAcc                               <  1:269603/250‑1 (MQ=255)                                                                                                                                                                                                                                          cacTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGCAGGTTTTAATCGCTATCAGAGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGa                 >  1:213560/1‑250 (MQ=255)                                                                                                                                                                                                                                                        tAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAagcagc  >  1:324645/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                           TGACCCGACGGCGGATTGCGGCGGTAGCCAGCTGGAAGGAACAACGTTGCCCAAAGCCTGAATGAGTATTGGGCATTTTGCCGGATGCGGCGTAAACGCCTTATCCGGCTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAA‑CGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCCGACACTATTCCTCAATTAAAAGAACACGACATACACCGCAGCCGCCACAATAAAGCGATAAATGGCGAACGGGATAAACGAAATGCGCTTAATCAATTGCAGGAAGGTTTTAATCGCTATCAGCGCCACCACAAAAGCGGTGATAAACCCAACGGCAAACATCGGGATATCGCCGCTTGTCAGGAAGCCCCAGCTTTTGTAGAGATCGAGCGCCGTTGCGCCCATCATCATCGGCACCGCCAGCAGGAACGAAAACTCGGAAGCAGC  >  NC_000913/3203065‑3203561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑