Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,172,897	G→A	intergenic (‑24/+1385)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,172,897	0	G	A	100.0%	43.1 / NA	13	intergenic (‑24/+1385)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

CCGGATCCCACCGCATCAATATAGCCGCTAAATTCATGGCCTAACGTTATTGGATAATAATGTGCACCATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGGATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAG  >  NC_000913/2172662‑2173136                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                ccGGATCCCACCGCATCAATATAGCCGCTAAATTCATGGCCTAACGTTATTGGATAATAATGTGCACCATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCa                                                                                                                                                                                                                                  <  1:6997/251‑1 (MQ=255)   ggATCCCACCGCATCAATATAGCCGCTAAATTCATGGCCTAACGTTATTGGATAATAATGTGCACCATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAgg                                                                                                                                                                                                                                >  1:146147/1‑251 (MQ=255)         cACCGCATCAATATAGCCGCTAAATTCATGGCCTAACGTTATTGGATAATAATGTGCACCATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTaaaa                                                                                                                                                                                                                           >  1:94093/1‑250 (MQ=255)                                                                gCACCATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTTGCAATTTTTACCCGCACCGCATCCTGATGTTTAAGTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTTTTTAATAAAGCCACGCGCGCTACGCCa                                                                                                                                                                    <  2:140138/250‑1 (MQ=255)                                                                    cATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTc                                                                                                                                                                >  1:209850/1‑250 (MQ=255)                                                                                               aaCCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCAc                                                                                                                                    >  2:7238/1‑251 (MQ=255)                                                                                                            cGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGa                                                                                                                        >  2:36198/1‑250 (MQ=255)                                                                                                                                                             aTGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATg                                                                      >  2:61281/1‑251 (MQ=255)                                                                                                                                                                     ttCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAAcc                                                              >  1:67155/1‑251 (MQ=255)                                                                                                                                                                             cgcgCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTACCCAGGTAATGGGAGAACCGCCCTGAt                                                      <  1:38064/251‑1 (MQ=255)                                                                                                                                                                                                                 tCATAAAAACTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCGGATCCAGTGAAGCCACCATACCCCCGGCTTTTAACGcc                   <  2:212679/250‑1 (MQ=255)                                                                                                                                                                                                                      aaaaCTCCTGATTGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCa             <  1:212631/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGTTAAGGGGAATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAg  <  1:72991/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                CCGGATCCCACCGCATCAATATAGCCGCTAAATTCATGGCCTAACGTTATTGGATAATAATGTGCACCATTTTTAAATATCCTGGGTAAATCGGAACCACATAAGCCCGAGCTGGCAATTTTTACCCGCACCTCATCCTGATGTTTAATTTCAGGAATGACGCTTTCTGCAACGCGCACGATACCATCAGTATCATTCACCACTGATTTCATAAAAACTCCTGATTGTTAAGGGGGATAACCTCCCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAG  >  NC_000913/2172662‑2173136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑