Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,011,066	A→G	N156S (AAT→AGT)	yedL →	putative acetyltransferase YedL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,011,066	0	A	G	100.0%	64.1 / NA	18	N156S (AAT→AGT)	yedL	putative acetyltransferase YedL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/12);  total (6/12)

GAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGCCCAGGACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACATTTAATATTTAATTATGAGTTCCTCACCATCTATTACATACTTTTTAACCATATCGGAATATTTATCATAATCAGCGGGATTCGTAACAAT  >  NC_000913/2010821‑2011312                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGCCCAGGACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGaa                                                                                                                                                                                                                                                    <  1:213679/250‑1 (MQ=255)                                   cgtGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGCCCAGGACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCAtt                                                                                                                                                                                                                  <  2:198862/250‑1 (MQ=255)                                                            gCCACATTTTGCAGGTCGCCCAGGACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCAc                                                                                                                                                                                        <  1:138943/251‑1 (MQ=255)                                                                                 aGGACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTccc                                                                                                                                                                   >  2:57897/1‑250 (MQ=255)                                                                                                aTCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCca                                                                                                                                                    <  1:214043/251‑1 (MQ=255)                                                                                                         gCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGcc                                                                                                                                            >  1:193506/1‑250 (MQ=255)                                                                                                                    ggTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACctc                                                                                                                                >  2:207996/1‑251 (MQ=255)                                                                                                                            ggCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAg                                                                                                                        <  2:73303/251‑1 (MQ=255)                                                                                                                                    ttACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTCTTCCAGTGAcaca                                                                                                                 >  2:18205/1‑250 (MQ=255)                                                                                                                                      aCGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAAtt                                                                                                              <  2:42160/251‑1 (MQ=255)                                                                                                                                             gCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTCTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCt                                                                                                        <  2:76058/250‑1 (MQ=255)                                                                                                                                               cATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGt                                                                                                                                                                                                            <  1:55570/148‑1 (MQ=255)                                                                                                                                               cATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGt                                                                                                                                                                                                            >  2:55570/1‑148 (MQ=255)                                                                                                                                                         aTTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGCCGCAGCACATTCTTGCATTCGACTTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACTCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACatt                                                                                           <  1:96811/251‑1 (MQ=255)                                                                                                                                                                             gATTGCGAACCGTTTGCTGATTATCGACGTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACATTTAATATTTAATTATGAGTTc                                                                       <  2:58051/251‑1 (MQ=255)                                                                                                                                                                                           tGCTGATTATCGACTTGGTCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATGCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACTCAATTGCGCCTAATTAATTACATTGAATATTTAATTATGAGTTCCTCACCATCTAtt                                                          <  2:92590/250‑1 (MQ=255)                                                                                                                                                                                                                                      aCGCTATGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACATTTAATATTTAATTATGAGTTCCTCACCATCTATTACATACTTTTTAACCATATCGGAATATTTATCATAATCAGCggg              >  1:35326/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTGCGAAAATAGTGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACATTTAATATTTAATTATGAGTTCCTCACCATCTATTACATACTTTTTAACCATATCGGAATATTTATCATAATCAGCGGGATTCg         <  1:18205/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aatagtGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACATTTAATATTTAATTATGAGTTCCTCACCATCTATTACATACTTTTTAACCATATCGGAATATTTATCATAATCAGCGGGATTCGTAACAat  >  1:9679/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GAAATCAATGCGTACCGCGCCAAATTATTTACGTCGTGGTGTCGCCAGTCTGATTTTACGCCACATTTTGCAGGTCGCCCAGGACAGATGCCTTCATCGCCTGAGCTTAGAAACGGGTACACAGGCTGGATTTACGGCCTGCCATCAACTTTATTTGAAGCATGGTTTCGCTGATTGCGAACCGTTTGCTGATTATCGACTTGATCCACACAGTCGATTTTTATCATTGACGCTATGCGAAAATAATGAATTGCCATGAGCCAGACGCAGCACATTCTTGCATTCGACGTGCTGCGCCTTTATTTATCACCAGCAGGAAACGCCTTGTCCATAGACGCCCCTTCCACATGCGCCACAAGAAACCTCTATTCCAGTGACACAATTGCGCCTAATTAATTACATTTAATATTTAATTATGAGTTCCTCACCATCTATTACATACTTTTTAACCATATCGGAATATTTATCATAATCAGCGGGATTCGTAACAAT  >  NC_000913/2010821‑2011312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑