Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,816,943	T→C	T65A (ACT→GCT)	chbG ←	chitooligosaccharide monodeacetylase ChbG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,816,943	0	T	C	100.0%	63.9 / NA	19	T65A (ACT→GCT)	chbG	chitooligosaccharide monodeacetylase ChbG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/10);  total (9/10)

CCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGG  >  NC_000913/1816699‑1817182                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                cccTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGAc                                                                                                                                                                                                                                           >  2:24196/1‑251 (MQ=255)                                                       acaTGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATg                                                                                                                                                                                     <  2:218485/251‑1 (MQ=255)                                                                           aTGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTc                                                                                                                                                                  >  1:32189/1‑250 (MQ=255)                                                                                                 aGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGa                                                                                                                                            <  2:176498/250‑1 (MQ=255)                                                                                                                                                                         gCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAAc                                                                   >  1:93129/1‑251 (MQ=255)                                                                                                                                                                             aCTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAAt                                                                >  1:70966/1‑250 (MQ=255)                                                                                                                                                                                gCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAg                                                             <  1:201653/250‑1 (MQ=255)                                                                                                                                                                                    gATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGc                                                        <  2:157961/251‑1 (MQ=255)                                                                                                                                                                                                cAGCACACCATCGCGGGTTAACCCCGGCATAGCAGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCACCCCTATGGCCAGACTCGGTTCCTCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATc                                             >  1:107794/1‑250 (MQ=255)                                                                                                                                                                                                 aGCACACCATCGCGTGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCTCCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCAt                                                                       <  1:224972/223‑1 (MQ=255)                                                                                                                                                                                                 aGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGcct                                                                       >  2:224972/1‑221 (MQ=255)                                                                                                                                                                                                 aGCACACCATCGCGGGTTAACCCCGGCAGAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAg                                           <  2:22081/251‑1 (MQ=255)                                                                                                                                                                                                        cATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCAGCGGCATTAACAATCAGTAAGCTTTCCATAATCAGCCTCGGt                                    <  2:138663/251‑1 (MQ=255)                                                                                                                                                                                                              gggTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGACAATAGCCTGCCCATTCACCAGTGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAAtgt                              >  2:161926/1‑251 (MQ=255)                                                                                                                                                                                                                   aaCCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCttt                         >  2:50906/1‑251 (MQ=255)                                                                                                                                                                                                                    aCCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTTACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCtttt                        <  1:112944/251‑1 (MQ=255)                                                                                                                                                                                                                    aCCCCGGCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGAATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCtttt                        <  2:4061/251‑1 (MQ=255)                                                                                                                                                                                                                         ggCATAGCTGTCAGTGGCTTACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCCCGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGTCATTCACAATCAGTAAGCGTTCCATAATCATCCTCGGTTAATGTGTttttttt                     >  2:147573/1‑248 (MQ=255)                                                                                                                                                                                                                                            tACCCATAGCAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCgg  <  1:157460/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAAAGTGCATCCCTATGGCCAGACTCGGTTCATCACGACTCAACTGCACCGCATGGTCAATAGCCTGCCCATTCACCAGCGCCGTCGTCGACGTGACAATCCCATTGCGACAGGCCTCGATAATGCCGTAGTTCTGACCTTTGCTTAAGCCAAAATCATCGGCATTAACAATCAGTAAGCGTTCCATAATCAGCCTCGGTTAATGTGCTTTTTTAAGCTCTGCGATGCAGTCGG  >  NC_000913/1816699‑1817182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑