Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,642,112	G→A	intergenic (+45/‑10)	cspF → / → ynfS	cold shock‑like protein CspF/protein YnfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,642,112	0	G	A	100.0%	57.8 / NA	17	intergenic (+45/‑10)	cspF/ynfS	cold shock‑like protein CspF/protein YnfS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/11);  total (6/11)

AATGACAGGAATTGTCAAAACCTTTGACGGCAAAAGCGGCAAGGGTCTTATCACCCCATCCGATGGTCGTATCGATGTCCAGCTTCATGTTTCAGCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATT  >  NC_000913/1641866‑1642337                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  aaTGACAGGAATTGTCAAACCCTTTGACGGCAAAAGCGTCAAGGGTCTTATCACCCCATCCGATGGTCGTATCGATGTCCAGCTTCATGTTTCAGCGCTCAAGCTCCGCGATGCAGAAGAAATTACCACCTTATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCTTCGTATCATACATGAagag                                                                                                                                                                                                                               <  2:27668/251‑1 (MQ=255)                  aaaCCTTTGACGGCAAAAGCGGCAAGGGTCTTATCACCCCATCCGATGGTCGTATCGATGTCCAGCTTCATGTTTCAGCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAAcccc                                                                                                                                                                                                              >  2:61362/1‑251 (MQ=255)                       tttGACGGCAAAAGCGGCAAGGGTCTTATAACCCCATCCGATGGTCGTATCGATGTCCAGCTTCATGTTTCAGCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCgtctg                                                                                                                                                                                                         >  2:235636/1‑251 (MQ=255)                                                      ccccATCCGATGGTCGTATCGATGTCCAGCTTCATGTTTCAGCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGTCCCATCGGATCACACATGAAGAGTGTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCGTTAAAAGc                                                                                                                                                                          <  2:119006/251‑1 (MQ=255)                                                                                                    cAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATAtaataa                                                                                                                             <  1:95163/250‑1 (MQ=255)                                                                                                          ccGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGa                                                                                                                        <  1:168291/249‑1 (MQ=255)                                                                                                                                              ggAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACa                                                                                   <  2:13485/250‑1 (MQ=255)                                                                                                                                                aaTTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCTGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACAttt                                                                                <  1:11684/251‑1 (MQ=255)                                                                                                                                                 aTTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACAtttt                                                                               >  1:67654/1‑251 (MQ=255)                                                                                                                                                        cGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATc                                                                        <  1:81422/251‑1 (MQ=255)                                                                                                                                                          gATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAg                                                                      <  2:178461/251‑1 (MQ=255)                                                                                                                                                                             ccTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCa                                                   <  2:13815/251‑1 (MQ=255)                                                                                                                                                                                          aTGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAg                                      <  2:203041/251‑1 (MQ=255)                                                                                                                                                                                                              atatTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCgg                  <  1:202585/251‑1 (MQ=255)                                                                                                                                                                                                                     aGCTTTAATTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATg              >  1:155641/1‑248 (MQ=255)                                                                                                                                                                                                                           aaTTTCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAAc     >  2:125748/1‑251 (MQ=255)                                                                                                                                                                                                                              ttCAGGCCCCATCGGATCACACATGAAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATATTAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACAtt  >  2:161893/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  AATGACAGGAATTGTCAAAACCTTTGACGGCAAAAGCGGCAAGGGTCTTATCACCCCATCCGATGGTCGTATCGATGTCCAGCTTCATGTTTCAGCGCTCAATCTCCGCGATGCAGAAGAAATTACCACCGGATTACGCGTGGAATTTTGCCGGATAAATGGTCTGCGTGGCCCTTCAGCTGCCAATGTTTACCTTTCATGAGCTATATTAAAGCTTTAATTTCAGGCCCCATCGGATCACACATGGAGAGTTTTTATGAATAACCCCGTCTGTCTTGATGACTGGTTGATTGGCTTTAAAAGCTTATGCTGTACTTTGGCCGTAATAGCTCTGCTAATAATATAATAAGCAGACTCATTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATT  >  NC_000913/1641866‑1642337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑