Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,452,943	C→A	A234S (GCG→TCG)	paaZ ←	fused 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase and oxepin‑CoA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,452,943	0	C	A	100.0%	81.2 / NA	25	A234S (GCG→TCG)	paaZ	fused 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase and oxepin‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/11);  total (14/11)

CAGAGCATCACTGACAGCATTAACCAATGCCTGCGGCACAATAATCCGCCGGATTGCCGTACATTTTTGCCCGGCTTTTGTGGTCATCTCACGCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGCTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCAC  >  NC_000913/1452698‑1453189                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cggggCATCACTGACAGCATGATCCAACGCCTGGTGCTCAATAATCCGCCGGATTGCCCGACATTTGTGCCCGGCTTTTGTGGTCAACTCACGCACAACTTCACGAATAAACAGCGCAAACGCCGGTTGATCCGGGGTGACATCTGCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGAGTTGGCGACGATATTTGGCTGAACGCGCAACAGCTTTCCCGTCGCGGATGAcc                                                                                                                                                                                                                                                   <  2:233569/247‑1 (MQ=255)      cATCACTGCCAGCATTAACCAATGCCTGCGGCACAATAATACGCCGGATTGCCGTACATTTTTGCCCGGCTTTTGTGGTCATCTCACGCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGt                                                                                                                                                                                                                                               >  1:192196/1‑250 (MQ=255)                                        aataatCCGCCGGATTGCCGTACATTTTTGCCCGGCTTTTGTGGTCATCTCACGCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCca                                                                                                                                                                                                            <  1:178323/251‑1 (MQ=255)                                                                 ttttGCCCGGCTTTTGTGGTCATCTCACGCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTTCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTTTCCGGTCGCCGATGACCCCGTGAAATTCACCACATCCTGGCTGGCTAGATGATTCAAGAAGTCGCCATCACTACCGCAGa                                                                                                                                                                                    <  2:222208/250‑1 (MQ=255)                                                                                  ggTCATCTCACGCACAACTTCACGAATAAACCGCGAAAACTCCGGTTGATCCGGGGTGCCATCTTCTCCCAGTACGCAGCAGTTCACGGAATCAGCTTCCATCGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCCGACCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCCGGCTGTCCAGATGATCAAACAAGTCGCCAGCACTACCGCAGATCCGACTACTTGCGCCtt                                                                                                                                                                  >  1:215510/1‑251 (MQ=255)                                                                                          cACGCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGa                                                                                                                                                             <  1:140128/248‑1 (MQ=255)                                                                                             gCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACa                                                                                                                                                          >  1:45818/1‑248 (MQ=255)                                                                                                      cACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCg                                                                                                                                              <  2:187238/251‑1 (MQ=255)                                                                                                               aCAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGAt                                                                                                                                     >  2:83729/1‑251 (MQ=255)                                                                                                                 aGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCCGTTAAGGGAATCAGCTTCCATAGTGACGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTAACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGAttt                                                                                                                                   >  2:72993/1‑251 (MQ=255)                                                                                                                 aGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGAttt                                                                                                                                   <  1:213061/251‑1 (MQ=255)                                                                                                                   cgcAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCa                                                                                                                                 <  1:72472/251‑1 (MQ=255)                                                                                                                         cTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATcc                                                                                                                           >  1:221357/1‑250 (MQ=255)                                                                                                                           caggtagatcctgtgtgccATCGTCGCCCAGTCAGCAGCAGTTCTGGGAATCAGCTGCCAGAGTGAAGGCGATAGAATTCGCGACGAGATTTGGCTTAACTCGCAACACCTGTCCGTCCTCCGATGTCCCCGTGAAACTCACCTCATCCTGGCTGTCCAGATGATCCAACAAGTCACCAGCACCACCACAGATCAGACTAATTGCGCCTTCTGTAACAAGACCACTAACGTAAATTTATTTCCCCATCg                                                                                                                           <  2:118553/231‑1 (MQ=255)                                                                                                                                        ggTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCGCTACCGCAGATCAGACTAATTGCGCCTTCGGGATCAAGACCTCTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTggg                                                                                                            <  1:150837/251‑1 (MQ=255)                                                                                                                                          tGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGcc                                                                                                          >  1:1854/1‑251 (MQ=255)                                                                                                                                                   cGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTa                                                                                                 <  2:62107/251‑1 (MQ=255)                                                                                                                                                         gTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGt                                                                                           >  1:118556/1‑251 (MQ=255)                                                                                                                                                                      cAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGAtggctg                                                                              >  1:236363/1‑251 (MQ=255)                                                                                                                                                                                                               cgaTATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCAtt                                     >  2:183069/1‑251 (MQ=255)                                                                                                                                                                                                                             aCTCGCAGCATCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGa                        >  2:205109/1‑250 (MQ=255)                                                                                                                                                                                                                                  cagcaTCTGTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCCGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCCGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTa                   >  1:231466/1‑250 (MQ=255)                                                                                                                                                                                                                                        cTGTCCGGGCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCAATATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGATAGCTTGTTTGATGATGGCTGGCCTTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCCTTCCCCAGCAGGGGAAGTTAAAGGCGt            >  2:129079/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTCCGGTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTGTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGc              <  2:116086/247‑1 (MQ=255)                                                                                                                                                                                                                                               gTCGCCGATGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCATTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAACGGCGTTAATATg     >  2:231975/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gccgaTGAGCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGCCGCCAGCACTACCGCAGATCAGACTAATTGCGCCATCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCTTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCACAGCTGGGGAAGTTAGAGGCGTTAATATGctc  >  2:188988/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CAGAGCATCACTGACAGCATTAACCAATGCCTGCGGCACAATAATCCGCCGGATTGCCGTACATTTTTGCCCGGCTTTTGTGGTCATCTCACGCACAACTTCACGAATAAACAGCGCAAACTCCGGTTGATCCGGGGTGACATCTTCGCCCAGTACGCAGCAGTTCAGGGAATCAGCTTCCATAGTGAAGGGGATAGATTTGGCGACGATATTTGGCTGAACTCGCAGCATCTGTCCGGTCGCCGCTGACCCCGTGAAAGTCACCACATCCTGGCTGTCCAGATGATCCAACAAGTCGCCAGCACTACCGCAGATCAGACTAATTGCGCCTTCGGGAACAAGACCACTATCGACAATTGATTTCACCATCGCCTGAGTCAGTTGGGCCGTCGCGGTAGCTGGTTTGATGATGGCTGGCATTCCGCCCAGCCACGTTGGTGCCAGCTTTTCCAGCATTCCCCAGCAGGGGAAGTTAAAGGCGTTAATATGCAC  >  NC_000913/1452698‑1453189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑