Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC96.0% 56.5 / ‑6.1 25Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (16/8);  minor base A (0/1);  total (16/9)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTG  >  NC_000913/1358619‑1359083
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                
gTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCCGGAAGCTAAACACAACCATGATGGAGATTTGCACCAGTACAAAAAGGGTATTGAAGTTCGCGCCCAGGTTCACGCTCTTCAGATTCGCGGCGGATAAAATGGCCACGAAGGGTTCCACCCACACCCAAGGCGGCACTTCCGGGAAGCGGGCGGAAAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagca                                                                                                                                                                                                                         >  1:38939/1‑250 (MQ=255)
gTGCCAACGCCTTCTCCTTTATGCAGGCCCTGAACGACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagcag                                                                                                                                                                                                                        <  2:201600/251‑1 (MQ=255)
                         gTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGGGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCCTAAATCCg                                                                                                                                                                                               >  1:38714/1‑251 (MQ=255)
                                aaCTACCAGGAAGATAAACACCACCATGAGGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTGCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGcc                                                                                                                                                                                        <  2:856/251‑2 (MQ=255)
                                      cAGGAAGATAAACAAAACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCAACGGCCAAAAGAGACAATCCAACAGCGGTGAACAGCCGACCAAAAAAACGACGTGCGGGTTa                                                                                                                                                                                   >  1:185643/1‑250 (MQ=255)
                                        ggAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATc                                                                                                                                                                                <  2:57967/251‑1 (MQ=255)
                                               aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtttt                                                                                                                                                                         <  1:24881/251‑1 (MQ=255)
                                                                    ttGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACCCCCCCGGCGGCCCCTCCGGGAAGAGGGCGGGGAGAAAAATTTTCGCCAACAAGACGTTAATCACCGGCAAAAAGAGACAAACCAGCAGCGATGACCAGCCGACCATAAAACCCACGGGCGGGTTTATCGACTTTTGCGCGTAgggaatagccgaac                                                                                                                                                    >  2:217893/1‑239 (MQ=255)
                                                                         ccAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGGGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGcc                                                                                                                                               <  2:41657/251‑1 (MQ=255)
                                                                                      ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGc                                                                                                                                  <  1:72804/251‑1 (MQ=255)
                                                                                      ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCCCTTCCGGGGAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCCTAAATCCGACGTGCGGGGTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTggg                                                                                                                                  >  2:175531/1‑250 (MQ=255)
                                                                                       gTATTGAAGTTAGCGACCCGGTTGACGCTCTTCAGATTCGCGGCGGTTGAAATTGCGACGAAGGGTACCACCCACACCCAAGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATGTTCGCCAACCAGACGTTAATCATAGGCAAAAAGAGACAATCCAGCAGCGAGGACCAGCCGACCCAAAATCCCACGTGCGGGTTAATCGACTTTTTCGCGTCGGTATAAGCCGAACCGGCCTCCCGGAACTGGCg                                                                                                                                 >  2:578/1‑251 (MQ=255)
                                                                                             aaGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCCCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGCAcc                                                                                                                             >  1:45742/1‑249 (MQ=255)
                                                                                             aaGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGAAAGATTTTTGCCAACAAGACGTTAATCATCGGCAAAAAAAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGGGCGGGGTAATCGACTTTTGCGCGTAGGGATAGGCCGGACCGGCCCCCGGAAACTGGCGAAcc                                                                                                                             >  1:10775/1‑249 (MQ=255)
                                                                                                ttAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGGAGAAAGGTACCACCCACCCCCCCGGCGGCACTTCCGGGGAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGGTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCCGCCGACCATAAATCCGACGTGCGGGGTAATCGACTTTTGCACGGAGGGATAGGCCGAAACGGCCACCCGAAACTGGGGaaaaaggtt                                                                                                                        >  2:101449/1‑244 (MQ=255)
                                                                                                   gCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCAACCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAAACCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGGAACTGGCGAACCAGTTTGcc                                                                                                                     >  1:190943/1‑251 (MQ=255)
                                                                                                                         gATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGGAGCTGATAGCGGTAAACAGc                                                                                               >  1:85614/1‑251 (MQ=255)
                                                                                                                                            ggCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCgccagcgccagc                                                                             >  1:135814/1‑250 (MQ=255)
                                                                                                                                                   aaGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAgg                                                                      >  2:183340/1‑250 (MQ=255)
                                                                                                                                                            acccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAAc                                                            >  1:116153/1‑251 (MQ=255)
                                                                                                                                                                         gcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCAAACAAGACGTTAATCATCGGCAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTa                                               <  1:175531/251‑1 (MQ=255)
                                                                                                                                                                                       aaGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACaa                                  >  2:162397/1‑250 (MQ=255)
                                                                                                                                                                                        agagGGAGGAAAAATAGATTTTCGCCAACAAGCCGTTAATCATCGGCAAAAAGAGACAACCCAGCAGCGATGCCCAGCCGACCATAAATCCGCCGTGCGGGTTAATCGACTTTTGCGCGTAGTTATAGGCCGACCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATa                                <  1:70068/251‑1 (MQ=255)
                                                                                                                                                                                              cGGAGAGATAGATTTTCGCCAACAAGACGATAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGACGCTAATGCCGGAGACAATACCaaa                           >  2:133083/1‑250 (MQ=255)
                                                                                                                                                                                                                      aGACGTAAATAAGCGGAAAAAAGAGAAAACCCAGCAGCGATGACCAGCCGACCATAAACCCGACGGGGGGTTTAATCGGTTTTTGGGCGTGGGTATAGGCCAACCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGGGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTg  <  2:104218/251‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                
GTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTG  >  NC_000913/1358619‑1359083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑