Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7360TG89.5% 52.0 / NA 19S325A (TCC→GCC) oppFmurein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (8/9);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCT  >  NC_000913/1306495‑1306978
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
aGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGGCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCtt                                                                                                                                                                                                                                           <  1:30936/251‑1 (MQ=255)
                          aCCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCATAGGGCGCCAAAACACGTCCTGTTCTGTAGGGGAGTTTCAGGCACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTaaaa                                                                                                                                                                                                                  <  1:149254/250‑1 (MQ=255)
                                                          ttGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTc                                                                                                                                                                                 >  1:74217/1‑251 (MQ=255)
                                                           tGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTc                                                                                                                                                                                 <  1:115305/250‑1 (MQ=255)
                                                            gATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGCAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCAc                                                                                                                                                                               >  2:136735/1‑251 (MQ=255)
                                                                           cATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCACAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGattat                                                                                                                                                                >  1:115779/1‑251 (MQ=255)
                                                                            aTACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGattatt                                                                                                                                                               <  1:17059/251‑1 (MQ=255)
                                                                                          tCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                                                                     >  1:196034/1‑247 (MQ=255)
                                                                                             ggAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                                                                <  1:140790/249‑1 (MQ=255)
                                                                                                    aaCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCa                                                                                                                                        >  1:175991/1‑250 (MQ=255)
                                                                                                                     ccTGGAAGGTGAATTCCCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTGCCGTACCCGTTGCCGGATTGCCGGTCCAGAGTGCGACAAAACACGTCCTGTTCTGGAGTGGAGTTTCAGACACGCCTTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                                                                                                                       <  2:204727/249‑1 (MQ=255)
                                                                                                                                         cGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTAAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCCTAACAGAATGCggg                                                                                                  >  1:211537/1‑251 (MQ=255)
                                                                                                                                                     cGCCTTGCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGAGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGACTGACAGATGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGTCAAAAAAATCGt                                                                                      >  2:92081/1‑251 (MQ=255)
                                                                                                                                                                                          tggcGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGTAAGTAATTTTCAGACATTCCGTTTCTTGCCTGAAAGGCGATCCGTTTTAAAAACAATAATGGCTGACAGTTGTCAGCCCTTGTTCGCGCTAAAAGCTATTATTTGTTCCCGCCAGATGATGTGGCAAAGTTGGTGATGTGTTTCGCGGCATTTTAGATTTCGGGCTAAAACATCGTTTATTTCACCATCTTCACTGTCCGCCAGACCAATca                                                  <  1:29787/247‑1 (MQ=255)
                                                                                                                                                                                                                  ccGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGGCCGGGtt                         <  2:89036/250‑1 (MQ=255)
                                                                                                                                                                                                                      cctgttagggagggtgtagtcatacacGCCGTTTCTTGCCTGAAAGTCGGTCCGCTTTAAAAACAATAAGGGCGGGCAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGTTATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCAGCTTCACTGTCCGCCAGCCCAATCACCACTTCGGCAAAAAAGTCCGGGTTCaa                      <  2:86901/227‑1 (MQ=255)
                                                                                                                                                                                                                         gttccggcggggAGATTTAGAAACGCCGTTTCTTGACTTAAAGTCGAGCCGCTTAAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTACCGCAAGATGATATGGCAAAGTTTGTGATTTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAACAGCTTCACTGTCCGCCAGACCAAGCACCTCTACGGCAAAAAAGTCCGGGTTCAAATc                   <  2:52622/242‑1 (MQ=255)
                                                                                                                                                                                                                          ttCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCg                  >  1:68239/1‑250 (MQ=255)
                                                                                                                                                                                                                                          cAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTTATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACGGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCt  <  1:13733/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
AGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACATGTTCCT  >  NC_000913/1306495‑1306978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: