Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,337	G→T	K424N (AAG→AAT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,337	0	G	T	100.0%	54.2 / NA	18	K424N (AAG→AAT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/13);  total (5/13)

GTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCG  >  NC_000913/1252140‑1252577                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCa                                                                                                                                                                                             <  1:233296/251‑1 (MQ=255)                    gTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTg                                                                                                                                                                          <  2:98217/251‑1 (MQ=255)                                          cgagcgTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCa                                                                                                                                                     >  1:53251/1‑250 (MQ=255)                                                  ctgcAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTc                                                                                                                                            >  2:183845/1‑251 (MQ=255)                                                      aGGTCCTTATATCGCCGTCACTTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCac                                                                                                                                          >  2:133735/1‑248 (MQ=255)                                                              atatCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGGGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGc                                                                                                                                 >  2:128792/1‑250 (MQ=255)                                                                  cGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGcaacaa                                                                                                                            <  2:228494/251‑1 (MQ=255)                                                                   gCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGTGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCaacaac                                                                                                                           <  2:229781/251‑1 (MQ=255)                                                                                                    cTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGCAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGGTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAAAGACCGCGGACCTCGCAATGCTGGTGGAACaaa                                                                                           >  2:211078/1‑250 (MQ=255)                                                                                                         ggAAGAATTTATTGGTGGCGAACGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTTGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCGGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTGAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAGCATAATCGtt                                                                                     <  2:38657/251‑1 (MQ=255)                                                                                                            agaaTTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTa                                                                                  <  2:24551/251‑1 (MQ=255)                                                                                                                     tggtggCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAg                                                                          <  2:15320/250‑1 (MQ=255)                                                                                                                           ggATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATAGCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTTGATGCGCGGCGTTTAATACCAATTTATGTCAAAGTGATTGCAACAACGATCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTAtt                                                                   <  1:36440/250‑1 (MQ=255)                                                                                                                                                  gCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAAtt                                            <  1:158475/251‑1 (MQ=255)                                                                                                                                                         gaggctgCTGGAATTGGCACACGTCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGATTTGATTCAGGTTATCAAGCAGCGGGTTATCACGCGACTGGATGCGCGGCTTTTAATACCAATTGATTTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCa                                        <  2:210049/242‑1 (MQ=255)                                                                                                                                                           gTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCg                                   <  1:11938/251‑1 (MQ=255)                                                                                                                                                                              cggcggCACGTTGTTTCTTGAAAATATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGTTTATCAAGCAGGGGTTTATCCCGCGACGGGATGCGCGGCTTTTAATACCAATTGATGTAAAATTTATTGCAACACCGACCGCGGACCTCGCATTGCGGTTGGAACAAAATCGTTTTATTCCCCAGCTGTATTCCGCTCTTCATGCATTTGAAATTCCCATCCCGCCTCTGCGTATGCGGCGTg                <  1:176885/251‑1 (MQ=255)                                                                                                                                                                                             tCTTGAAAATATTGAATATCGGGCGGGGGAGTTTCAGTCTGCTTTGCTGCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATCCCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGAAACAAAATCGTTTTAGTCGCCGGCGGTATTACGCGCGGCATGAATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGgcg  <  2:170692/250‑1 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCG  >  NC_000913/1252140‑1252577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑