Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,620	T→A	V245V (GTT→GTA)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,620	0	T	A	100.0%	40.0 / NA	14	V245V (GTT→GTA)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/6);  total (8/6)

GTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCT  >  NC_000913/1098408‑1098842                                                                                                                                                                                                                     |                                                                                                                                                                                                                               gTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCtt                                                                                                                                                                                          <  1:205983/251‑1 (MQ=255)   gCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTAc                                                                                                                                                                                        <  1:59653/251‑1 (MQ=255)                   cGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGCTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGct                                                                                                                                                                        >  1:145469/1‑251 (MQ=255)                                                          gtGATTAATTGGTTACCGAATACACCTGAAACCGTCGGCATTATTAATCAACAATTTCTCTAAAACTTACCGGATGGCGCTTTTCTCCTCATCCTGGCGCGTGGGGTTCATGTTGTGCAAGATTACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAAAGTTGGATGTCTCTAATCGTGACCCCTTACCGCCTGAGAGTCCGCGCTGGCAACATCCCCGCGGGACGATCGCACCACATGTct                                                                                                                                  <  2:134721/249‑2 (MQ=255)                                                                aaTTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAACGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGAt                                                                                                                           >  1:35664/1‑251 (MQ=255)                                                                     gTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTAccc                                                                                                                      >  1:205082/1‑251 (MQ=255)                                                                               aCCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTg                                                                                                             >  1:32670/1‑250 (MQ=255)                                                                                        aCCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTgg                                                                                                    >  1:195595/1‑250 (MQ=255)                                                                                             cGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTAc                                                                                               <  2:79941/250‑1 (MQ=255)                                                                                                                                                cctcAACCTGGCGCGTGGAGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGAGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAAAACCACATGTCGCCGCGATTACCAGTCCCGCTGAAGCTGTGGCGTACATTTCTCGCACCATTGCACAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCa                                             >  2:150697/1‑249 (MQ=255)                                                                                                                                                      ccTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGAcc                                     <  2:156422/251‑1 (MQ=255)                                                                                                                                                                     tCATGTTGTGCAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAACAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGctact                      >  2:84350/1‑251 (MQ=255)                                                                                                                                                                               gAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCAt            >  1:73105/1‑251 (MQ=255)                                                                                                                                                                                          gCTCGCGGCGCTGGATAGCGGCAAAGTAAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCt  <  2:29991/250‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                               GTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTACCGAATACCCCTGAAACCGTCGGCATTATTAATCAACAATTACTCGAAAAATTACCGGATGGCGCGTATCTCCTCAACCTGGCGCGTGGTGTTCATGTTGTGGAAGATGACCTGCTCGCGGCGCTGGATAGCGGCAAAGTTAAAGGCGCAATGTTGGATGTTTTTAATCGTGAACCCTTACCGCCTGAAAGTCCGCTCTGGCAACATCCACGCGTGACGATAACACCACATGTCGCCGCGATTACCCGTCCCGCTGAAGCTGTGGAGTACATTTCTCGCACCATTGCCCAGCTCGAAAAAGGGGAGAGGGTCTGCGGGCAAGTCGACCGCGCACGCGGCTACTAATAAAGCATCAGGATTCCT  >  NC_000913/1098408‑1098842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑