Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,092,247	C→A	A15S (GCT→TCT)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,092,247	0	C	A	100.0%	81.6 / NA	25	A15S (GCT→TCT)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/14);  total (11/14)

TTGCTGATGACGGTAGCGGTTGTAAACGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGAGCAGAGTGTCCATATAAAATCCTTTTCTCGCCCGAAAATC  >  NC_000913/1092005‑1092474                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    ttGCTGATGACGGTAGCGGTTGTAAACGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTgg                                                                                                                                                                                                                             >  1:146122/1‑251 (MQ=255)             gTAGCGGTTGTAAACGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCAcc                                                                                                                                                                                                                 <  2:63494/251‑1 (MQ=255)               aGCGGTTGTAAACGGTAATACCCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTCAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCtt                                                                                                                                                                                                               >  1:182904/1‑251 (MQ=255)                       taaaaggcgataaCCTATTTATCTTGCCCGGCCTAAACGTTAATCTGTACCCAGTTAGCAATTTGGTTATTGCTGAGTGCTCATTTTGGTGCAAACCCTGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATACATCATAGGTGTTATTAACAGCACTCTTCGCCGCAGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCgggacactttttatgct                                                                                                                                                                                                       >  2:11772/10‑237 (MQ=255)                        aaaCGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGtttt                                                                                                                                                                                                                         <  2:156605/232‑1 (MQ=255)                        aaaCGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGtttt                                                                                                                                                                                                                         >  1:156605/1‑232 (MQ=255)                        aaaCGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCt                                                                                                                                                                                                       >  2:62631/1‑250 (MQ=255)                         aaCGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTAc                                                                                                                                                                                                     >  2:47012/1‑251 (MQ=255)                                                 cccGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTTCCCCTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCa                                                                                                                                                                             <  1:197511/251‑1 (MQ=255)                                                   cGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATg                                                                                                                                                                           <  1:27956/251‑1 (MQ=255)                                                              gccaacTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGCTTTTAGTGCAAACCATGACAAAGATGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGAAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTAATTGAATACATCCTGTATTACTCTATGTATTGCCaaa                                                                                                                                                                 >  2:1543/6‑250 (MQ=255)                                                                                      tGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAg                                                                                                                                         <  2:152737/250‑1 (MQ=255)                                                                                              gCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGa                                                                                                                                 >  1:176485/1‑250 (MQ=255)                                                                                                                                    gctgAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTaaa                                                                                          <  1:23201/251‑1 (MQ=255)                                                                                                                                       gAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcgc                                                                                       <  1:121192/251‑1 (MQ=255)                                                                                                                                             tACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAATTGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGa                                                                                    >  2:52702/1‑248 (MQ=255)                                                                                                                                                  ttGCGAGCTTCAATAATCAATGCATCATAGGCGCTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGAc                                                                            >  2:100015/1‑251 (MQ=255)                                                                                                                                                             aataatCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAAttgttg                                                                 <  1:62631/251‑1 (MQ=255)                                                                                                                                                                           catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGagaagaa                                                    <  2:146122/250‑1 (MQ=255)                                                                                                                                                                              aGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCTATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAgaagaagaa                                                 >  2:168312/1‑250 (MQ=255)                                                                                                                                                                                                                 gctgctAAAAATGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGAGCAGAGTGTCCATATAAAATCCTTTtctc             <  2:182904/251‑1 (MQ=255)                                                                                                                                                                                                                          aaTGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGAGCAGAGTGTCCATATAAAATCCTTTTCTCGCCCGaaaa    <  2:11450/251‑1 (MQ=255)                                                                                                                                                                                                                           aTGCGGCAGTAAGAAGTTTCAATGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACAGCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAAATGAAAAAAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATTATGTGAATAAAAAATGATGAGCATAGTGTCCATATAAAATCCTTTTCTCGCCCGAAAAt   <  2:210770/251‑1 (MQ=255)                                                                                                                                                                                                                           aTGCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTGTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGAGCAGAGAGTCCATATAAAATCCTTTTCTCGCCCGAAAAt   <  2:210761/251‑1 (MQ=255)                                                                                                                                                                                                                             gCGGCAGTAAGAAGTTTCAAAGACCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGAGCAGAGTGTCCATATAAAATCCTTTTCTCGCCCTAAAATc  <  1:162230/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    TTGCTGATGACGGTAGCGGTTGTAAACGGTAATAACCTGTTTATCTTGCCCGGCCCATAAGGCAATCTGTAACCAGTCAGCAATTTGGTTATTGCTGAGTGCTGATTTTAGTGCAAACCATGACAAAGCTGGCTGAGTATTACCCTTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAGAAGAAATGATGAGCAGAGTGTCCATATAAAATCCTTTTCTCGCCCGAAAATC  >  NC_000913/1092005‑1092474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑