Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,078,638	G→T	A82D (GCC→GAC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,078,638	0	G	T	100.0%	68.9 / NA	21	A82D (GCC→GAC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/12);  total (9/12)

CGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGGCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCC  >  NC_000913/1078391‑1078864                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   cgcCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCaa                                                                                                                                                                                                                                 <  2:17963/251‑1 (MQ=255)   ccACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAg                                                                                                                                                                                                                               <  1:120797/251‑1 (MQ=255)        ccTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCGGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATGTGCTCGTCAAAGTCGAg                                                                                                                                                                                                                          <  1:50837/251‑1 (MQ=255)            ccTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGaa                                                                                                                                                                                                                       <  2:50932/250‑1 (MQ=255)                    gACAGCGAAAAATCCTGCAATAAACCCTCGAGAAAACCTGCGCGACCCCTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTACGCCTGTTCAGCAACTGCCTGCGGCAGGCGGGGTTGTTCCAGCAGTCTAGAAAACGCTTCGGCTTCCGGGAGGCGCTTGGGCGGGGTGACAGAGCCGCGGGAAACCGACTAGGGCAATATTTGCTCCTCAAAGTGGACGAATGGCTgcgg                                                                                                                                                                                                              >  2:150122/1‑248 (MQ=255)                                tCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGGGGTTCCTCTGc                                                                                                                                                                                                  >  1:72666/1‑251 (MQ=255)                                         aaCCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCCATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGGGGTTCCTCTGCCGGAGTCgg                                                                                                                                                                                         >  1:128503/1‑251 (MQ=255)                                         aaCCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCg                                                                                                                                                                                          <  1:74859/250‑1 (MQ=255)                                         aaCCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATAAGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCg                                                                                                                                                                                          <  1:102442/250‑1 (MQ=255)                                                 gACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCAt                                                                                                                                                                                 <  1:170671/251‑1 (MQ=255)                                                                                                 gtccAGCGGATACTCTAGTTTGGGCGCCTGTGCAGCAACGAGCTGCGGCAGGCGGGCTTGTTCCCACGGCATACAACCCGCATCGGTTTCCGGGTGGCGATAAGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGTTTCCTCTGCCGGAGTCGGTGCTTCATCGGTCTCATTGGCCGCGCCAGAAATCAGCGCAGGTAGCTCCGGCagag                                                                                                                                 <  2:109666/249‑1 (MQ=255)                                                                                                     aGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATc                                                                                                                             <  1:195073/251‑1 (MQ=255)                                                                                                                           ccTGTTCAGCAACTGCCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCCGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCCGGTGCGGTTCCTCTGACGGAGTCGGTGCTTCATCGCCTTCATTTGCCGCGCCAGAAAACAGCGCAGGGAGCTCCGGCAGAGTATCGCTGTTTTCCCGTTGTTCggg                                                                                                        >  2:205759/1‑248 (MQ=255)                                                                                                                               ttCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATaa                                                                                                    <  2:10982/250‑1 (MQ=255)                                                                                                                                                                 agcagcATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAgtgtggtgt                                                                 <  1:200287/251‑1 (MQ=255)                                                                                                                                                                   cagcaTAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGc                                                               >  1:205274/1‑251 (MQ=255)                                                                                                                                                                         aGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGcgatcg                                                          <  1:207633/250‑1 (MQ=255)                                                                                                                                                                            aaCCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCCGAAAGCAGCGCAGGTAGCTCCGGCAGAGTAACGCTGGTTTCCAGTTGTTCTCGATAAGAAAAAATCGCCTGCTTAATTTACCAGTGTGGTGTGCgatagata                                                       >  2:31134/1‑250 (MQ=255)                                                                                                                                                                                                ggcgATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAAt                                   >  2:61237/1‑250 (MQ=255)                                                                                                                                                                                                          cgcgGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCAcgcg                        >  1:28533/1‑251 (MQ=255)                                                                                                                                                                                                                                aCCGACTGGGGCAATATTTGCTCGTCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAAcccc  >  1:220632/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   CGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCTCGGCAAAGTCGAGGAATGGCTGGTGTGGTTCCTCTGCCGGAGTCGGTGCTTCATCGCTCTCATTGGCCGCGCCAGAAAGCAGCGCAGGTAGCTCCGGCAGAGTATCGCTGTTTTCCAGTTGTTCGAGATAAGAAAAAATCGCCTGCTTAATTAACCAGTGTGGTGTGCGATCGATACGTGTCGCGGCAGACTTAATACGCTCACGCGTCGCGTCGTCCAGCTTAACCCC  >  NC_000913/1078391‑1078864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑