Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,126	G→T	L277L (CTG→CTT)	appB →	cytochrome bd‑II subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,126	0	G	T	100.0%	63.8 / NA	23	L277L (CTG→CTT)	appB	cytochrome bd‑II subunit 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/12);  total (11/12)

GGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTACT  >  NC_000913/1039901‑1040370                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ggCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGGTTTGTAATGCTCGCCAAGGGTGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCCGTGCTACCTGGTGCCTGGGTGAATAATTTTGTCGAGTCGCCCGCTTTGTGGAT‑CTTCCCGCTGCTGGGCTTCTTCTTCCCCTTGCTTACGGTGATGGCGTTTTCTCGGGGTc                                                                                                                                                                                                                               >  1:98214/1‑251 (MQ=255)     aCCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGccc                                                                                                                                                                                                                           <  1:128970/251‑1 (MQ=255)     aCCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGccc                                                                                                                                                                                                                           <  1:194561/251‑1 (MQ=255)          aCGCGGTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTGCTGCCTCTGGGTCGCGATTGGTGGCTTTGTGCTGGTAGCCCAGGGTGCTTAACGG‑CCTTCCAATCCGTTAATGAAACTGGTGCCCGGGATACCTGGGGACCGGGCGGATCATTTTTTCGCGTCGCCCGTTTTGTGGATGTTTCCCGC‑GTTGGGATTCTTATGCCCACTGCTTACGATGACGGCGAATTTCCGTGGACGCCC‑AGGtt                                                                                                                                                                                                                     >  2:141988/1‑251 (MQ=255)             cgcTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTTCTCGCCCAGGATGCT‑AACGGTCCTTCCCATCCGTTTATGAAACTGGTGGCAGTGCTACCTGGGGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGGTTCTTCTGCCCCTTGCTTTCGGTGATGGCGCTTTATCGTGGTCGCCCCGGGTTgg                                                                                                                                                                                                                   >  2:199327/1‑251 (MQ=255)                         ggTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGGGGGTCGGGATTGATGGCTTTGTACTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGGGGCAGGGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATgg                                                                                                                                                                                                      >  1:55450/1‑251 (MQ=255)                                  gtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGGATTGATGGCTTTGTACTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGGGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTTCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGGTTTTTGTTGGCATCATTg                                                                                                                                                                                              >  1:87194/1‑250 (MQ=255)                                                               gggTCGGTATTGATGGCTTTGTGCTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGGCTAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGAGTTTTGAAGGCATCATTGATGCAATTTGGCGTGATGTTCACggcaggc                                                                                                                                                                <  1:27227/251‑1 (MQ=255)                                                                                                                           ttAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGtt                                                                                                     >  1:204551/1‑251 (MQ=255)                                                                                                                                gAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCCGCCTGACGTTGTggg                                                                                                >  2:174614/1‑251 (MQ=255)                                                                                                                                  atatggttgctgTGCTACCTGGTGCCTTGATGAATAATATTGTCTAGTCGCGCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCTATGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGAGGCAATTTGGCGTGATTTTCACGGCAGGCATCGCGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTACTATCTCTAGCAGGACGTTGTgggtc                                                                                              <  2:97670/240‑3 (MQ=255)                                                                                                                                  aaCTGGTGGCAGTGCTGCCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTTCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTGGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGAc                                                                                              <  1:153993/251‑1 (MQ=255)                                                                                                                                          gCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTc                                                                                      >  1:174828/1‑251 (MQ=255)                                                                                                                                                  aCCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGc                                                                              <  1:219071/251‑1 (MQ=255)                                                                                                                                                   ccTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTAGCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTTATGCAATTTGGCGTGATTTTCACGGCAGGCATCACTCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGATAGTACTTCCAGTCAGCt                                                                             <  1:226898/251‑1 (MQ=255)                                                                                                                                                          ccTGGCTGAATAATTTTGTCGCGTCGCTCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCCTTCCGTCCAGCGTGAGTCCGATCTCCAGCCTGACGTTGGGGGACAGTACTTCCAGTCAGCTG‑ACGCTg                                                                     >  2:96045/1‑251 (MQ=255)                                                                                                                                                                     aatTTTGTCGCGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCAGTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGTGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGTCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAG‑TGTACTCTGATCATTATGtt                                                          <  2:95727/251‑1 (MQ=255)                                                                                                                                                                      atTTTGTCGAGTCGCCCGTTTTGTGTAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTg                                                         <  1:101995/251‑1 (MQ=255)                                                                                                                                                                          tGTCGAGTCTCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTTCCGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATGTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGAGCTCCAGCCTGACGTTGTGGTACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGtc                                                      <  2:52152/250‑2 (MQ=255)                                                                                                                                                                                                      cccGCTGCTGGGATTCTGCTGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTgtgt                          <  2:122863/251‑1 (MQ=255)                                                                                                                                                                                                                      ttctGCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCCGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTg          >  1:103708/1‑251 (MQ=255)                                                                                                                                                                                                                          gCCCATTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGc      <  2:75498/251‑1 (MQ=255)                                                                                                                                                                                                                              aTTGCTTACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGctact  >  2:59939/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCT‑AACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGAT‑CTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCC‑GGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGCGTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCGATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTG‑ACGCTGAGCATTATGTTGGTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTACT  >  NC_000913/1039901‑1040370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑