Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,031,775	T→G	intergenic (‑63/‑364)	serT ← / → hyaA	tRNA‑Ser/hydrogenase 1 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,031,775	0	T	G	100.0%	71.4 / NA	21	intergenic (‑63/‑364)	serT/hyaA	tRNA‑Ser/hydrogenase 1 small subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/12);  total (9/12)

GGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCTGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTG  >  NC_000913/1031541‑1032011                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             ggTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAg                                                                                                                                                                                                                              <  1:64492/251‑1 (MQ=255)  gTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTCAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGt                                                                                                                                                                                                                             >  2:71172/1‑251 (MQ=255)        aaGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCAtt                                                                                                                                                                                                                        <  1:83307/250‑1 (MQ=255)                ataACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGGGCATTGATAGCGTg                                                                                                                                                                                                               >  1:200766/1‑251 (MQ=255)                                ttCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGAtt                                                                                                                                                                                                <  1:61149/250‑1 (MQ=255)                                           aaaGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTtcatc                                                                                                                                                                                    <  1:26589/251‑1 (MQ=255)                                                          aGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCTCAGAGATTCGAACTCTGGAACCCTTTCGGGTCTCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTtc                                                                                                                                                                     <  1:230906/251‑1 (MQ=255)                                                                  gCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTAc                                                                                                                                                             <  2:57643/251‑1 (MQ=255)                                                                               cttatTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAAcc                                                                                                                                                >  2:62336/1‑251 (MQ=255)                                                                                  atTTAGCGGAAGCGCAGAGATGCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGCCCGGTGCCTTCAACCGCTCGGCCACACTTCCTGAATTACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTGTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGc                                                                                                                                             <  2:142267/251‑1 (MQ=255)                                                                                         ggAAGCGCAGAGCTTCGAAATCTGGAACAATTCCGCGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCCCACTTCCGGAATGACGCGCACTATAACCATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCCTTTCATCACCATGAATTCCTTCTCTTTTAATTGTTTAGCAACCGGCTAAACAt                                                                                                                                      >  2:121013/1‑251 (MQ=255)                                                                                                              ctGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCaaa                                                                                                                    >  2:215205/1‑248 (MQ=255)                                                                                                                            ggTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACAGCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTCCTCGTTTAGCAACCGGCTAAACATCCCCCCCGCCCGGCCAAAAGAAAAATAGGTCCAtt                                                                                                   <  1:241357/251‑1 (MQ=255)                                                                                                                                gCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCAttttt                                                                                                >  1:19792/1‑250 (MQ=255)                                                                                                                                               cGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATaaa                                                                                <  1:115570/251‑1 (MQ=255)                                                                                                                                                                      cacTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTtg                                                         <  1:63066/251‑1 (MQ=255)                                                                                                                                                                                              aaaCATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTttat                                 <  1:219766/251‑1 (MQ=255)                                                                                                                                                                                                          gcggcgTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGGGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACttt                     >  2:159854/1‑251 (MQ=255)                                                                                                                                                                                                           cggcgTGTAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTc                    >  1:67198/1‑251 (MQ=255)                                                                                                                                                                                                                 gtAAACCCCTAATTTGTTTGTTTGCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCg               >  2:126080/1‑250 (MQ=255)                                                                                                                                                                                                                              ttgtttgtttgCCGGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTg  <  1:208800/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             GGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACCCCTTATCTTATTTGGCGGAAGCGCAGAGATTCGAACTCTGGAACCCTTTCGGGTCGCCGGTTTTCAAGACCGGTGCCTTCAACCGCTCGGCCACACTTCCGGAATGACGCGCACTATAAACATCCCGATGCGGCGTGTAAACCCCTAATTTGTTTGTTTGCCTGAAAAACAGCCAAAAGTGCATTGATAGCGTGAAATAACAGCAGATTGATCATTTCATCACCATGAATTCCTTCTCTTTTACTCGTTTAGCAACCGGCTAAACATCCCCACCGCCCGGCCAAAAGAAAAATAGGTCCATTTTTATCGCTAAAAGATAAATCCACACAGTTTGTATTGTTTTGTGCAAAAGTTTCACTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTG  >  NC_000913/1031541‑1032011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑