Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	968,837	G→A	D158N (GAT→AAT)	lpxK →	tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	968,837	0	G	A	100.0%	52.7 / NA	17	D158N (GAT→AAT)	lpxK	tetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/12);  total (5/12)

ATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTA‑CCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTG  >  NC_000913/968611‑969080                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aTCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGGGATGAACCTGTGTTGATTTTTCAACACCCTGATGGGCCTGTTGGGGGTTTTCCCGTTCGTTCTGATGTGGGCACAGCCCCTCTGGCGCA‑CACCCCCGGTGTGGAGATCATCGGAACCAACGACGGTTTACAGCCGTAACGTCTGTGCGCG‑AATGGGGGAATTGTCGTTTTTGATg                                                                                                                                                                                                                              >  2:190992/1‑251 (MQ=255)   cgcgGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATgg                                                                                                                                                                                                                             <  2:223895/250‑1 (MQ=255)                                                         ccACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTgagcga                                                                                                                                                                      <  1:16264/251‑1 (MQ=255)                                                                     aGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGTGCGCTTa                                                                                                                                                          <  2:139430/251‑1 (MQ=255)                                                                           gTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCg                                                                                                                                                    <  1:125562/251‑1 (MQ=255)                                                                                   ccTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCTCTTAAAGTCGGTTGATGc                                                                                                                                            <  1:197281/251‑1 (MQ=255)                                                                                      gtgtTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGGCAATGCGGGGGCGAGCGGGGGGCTTACAGttgggtggtggggg                                                                                                                                         >  2:214444/1‑238 (MQ=255)                                                                                           gATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGCTGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGCAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGGCCATGCGTGAGGGGGCCGGGGGCTTCAAGCCGGTTTGTGCGGttgtcg                                                                                                                                    >  2:172406/1‑246 (MQ=255)                                                                                                                    gTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACTCCCT‑GATGTGCAGATCATCGTAACCGACGACGGGTTACAGCATTACCGTCTG‑GCGCGTAATTTGGAAATTGTCGTTATTGATGGTGTGCGTCGCGTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGGCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCg                                                                                                            <  1:40652/250‑1 (MQ=255)                                                                                                                                                        gCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGGGTCCCTCGCCGCGGTGAAATCCCCCTGCCTCTGCTGCCGGGTCAGGCggg                                                                       >  1:225085/1‑250 (MQ=255)                                                                                                                                                           aTTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCTGTGaa                                                                    <  2:32638/251‑1 (MQ=255)                                                                                                                                                                                 gtgCAGAGCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATGTACGTA‑CCGGTACGCGtt                                               <  2:40959/250‑1 (MQ=255)                                                                                                                                                                                     aGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTA‑CCGGTACGCGTTGTGAc                                          <  1:12590/251‑1 (MQ=255)                                                                                                                                                                                       atcatcGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTTGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCTAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTA‑CCGGTACGCGTTGTGACGt                                        <  1:4001/251‑1 (MQ=255)                                                                                                                                                                                             gTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCCACGGCGGGGTCCCTCACAGCGGGGAAATCCCCCTGCAACTGCTGCCGGGTCAGGGGGGGGAGTTACGTAACCGG‑ACGCGTTGTGTCGTTGCTcc                                  >  1:28987/1‑250 (MQ=255)                                                                                                                                                                                                                             g‑gCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTA‑CCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGAt    <  1:20465/249‑1 (MQ=255)                                                                                                                                                                                                                             g‑gCGCGTAATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGGCAGTCGGTGAATTTACGTA‑CCGGTACGCTTTGTGACGTTTCTCAGCTTGAACATGTAGTCGCGATGTCGGGGATTg  <  2:113352/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGATACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCTGTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCT‑GATGTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTG‑GCGCGTGATGTGGAAATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCAATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTCCCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTA‑CCGGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTG  >  NC_000913/968611‑969080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑