Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	905,735	C→T	Q275* (CAG→TAG)	amiD →	N‑acetylmuramoyl‑L‑alanine amidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	905,735	0	C	T	100.0%	84.9 / NA	27	Q275* (CAG→TAG)	amiD	N‑acetylmuramoyl‑L‑alanine amidase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

CCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCATCGGGTAGGGGACGGAAC  >  NC_000913/905495‑905975                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ccGGATGCGCAGCGGGTGAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGAGACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAATCCTGAGATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGAGGCATTTCCGCCCGACGTTATATAACGTCGAAGCGGATGCAGAAACGCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGTTAGGATTAgcg                                                                                                                                                                                                                                        <  2:213136/251‑1 (MQ=255)      tGCGCAGCGGGTTAACTTTTACCTTGCCGCGCGCGCGCCGCACACACCGGTAGGTACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCAAGATGCATTTCCGCCCGCCGTTATATAACGGCGAAGCGGATGCAGAAACTCCGGCGATTGCAGAAGCATTGCTGGAGAAATACGGGTAGGATTCGCGCGGc                                                                                                                                                                                                                                    >  2:224698/1‑250 (MQ=255)                     cTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTcgc                                                                                                                                                                                                                    >  1:165941/1‑251 (MQ=255)                           ccTTGCCGGGCGCGCGCCGCACACACCGGTAGATACAGCGTCACTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGcc                                                                                                                                                                                                              <  2:227256/251‑1 (MQ=255)                                 cGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg                                                                                                                                                                                                        >  1:169291/1‑251 (MQ=255)                                 cGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg                                                                                                                                                                                                        <  1:8546/251‑1 (MQ=255)                                          gccgcACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCACGTAGCCAGGCGGCAGTTTtct                                                                                                                                                                                               >  1:9121/1‑251 (MQ=255)                                                               cgcgTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgg                                                                                                                                                                          >  2:105037/1‑250 (MQ=255)                                                                                    ggCGTGCTAAGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct                                                                                                                                                     >  2:156856/1‑251 (MQ=255)                                                                                        cgcTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCTTCCAGGGTGATGACCGGCTGATAACCTAACTCTTc                                                                                                                                                 <  1:68675/251‑1 (MQ=255)                                                                                                 tATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAcg                                                                                                                                        >  1:167527/1‑251 (MQ=255)                                                                                                        ttAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGGTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTACCTCTTCCTGCGCACGCGTAat                                                                                                                                  >  2:20318/1‑250 (MQ=255)                                                                                                          aaaCCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATc                                                                                                                               <  2:107651/251‑1 (MQ=255)                                                                                                                  gatgtggccgagcgAGCAGCGGCGCGTGATTATGGCATTCCCGATGCATTTCAGCACGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGCTTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGAAGTGTTGTCGTTACCTTCATCTAGGGCGATGACCGGCTGCTAACCGTAGTCTTCCTGCGCACGCGTAATATCCAGCGTa                                                                                                                        >  2:32823/12‑250 (MQ=255)                                                                                                                       cacCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTc                                                                                                                   >  2:222174/1‑250 (MQ=255)                                                                                                                                   agcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTg                                                                                                      <  2:41296/251‑1 (MQ=255)                                                                                                                                                     cATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTc                                                                                    >  1:190796/1‑251 (MQ=255)                                                                                                                                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                                                                             <  1:105037/251‑1 (MQ=255)                                                                                                                                                                                   ataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCGTTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGAGCACGCGTAATATCCAGCGTAAAGTCAAAATGCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGcc                                                       <  2:46413/250‑1 (MQ=255)                                                                                                                                                                                                ggATGCAGAAACGCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGTAGGAGTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATAGCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGGGGCGGCTCTTGTGCTGACTTGCGGCCTAAACGCTCCAtgc                                         <  2:233327/251‑1 (MQ=255)                                                                                                                                                                                                                   aTTGCCGAAGCATTGCTGGAGAAAGACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGGTGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTGTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGGGATCATATCCAg                      <  2:239617/251‑1 (MQ=255)                                                                                                                                                                                                                         gAAGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTTCGGCCTAAAAGCTCCAATCCGCGGGCGATCCTATCCAGCATCgg                >  2:190686/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCATTGCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCATCGGGt              >  1:8702/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCATCGGGTAgggg         <  1:162842/250‑1 (MQ=255)                                                                                                                                                                                                                                  cTGGAGAAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTTCTGACTTGCGTCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCATCGGGTTGGGGAc       >  2:60904/1‑251 (MQ=255)                                                                                                                                                                                                                                      agaAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCATCGGGTAGGGGACGGaa   <  1:170646/251‑1 (MQ=255)                                                                                                                                                                                                                                       gaAATACGGGTAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCAGCGGGTAGGGGACGGAAc  <  1:173185/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 CCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTGCGGCCTAAACGCTCCATGCTGCGGGCGATCATATCCAGCATCGGGTAGGGGACGGAAC  >  NC_000913/905495‑905975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑