Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,240	G→A	L21L (TTG→TTA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,240	0	G	A	100.0%	106.2 / NA	30	L21L (TTG→TTA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

AAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAAC  >  NC_000913/4637993‑4638474                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           aaaGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTGCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAATACTATGTTGAAGTCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAAtt                                                                                                                                                                                                                                         <  1:31310/251‑1 (MQ=255)         ggTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAAt                                                                                                                                                                                                                                 >  2:170132/1‑251 (MQ=255)              ggggTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCt                                                                                                                                                                                                                              <  2:122918/249‑1 (MQ=255)               gggTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGAt                                                                                                                                                                                                                           >  1:141308/1‑251 (MQ=255)               gggTAGGCGTTCGTCAATGAGGTCGCCCGTTTGTTTGACGACGAAGTAAAGCTGCTCAACGTTCATGAAGGGGGCGTGCTGGCCTCGCTTATACTGCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGGAATCCCCCCTGTGCTGGAAAATGACTAGCCTGTTTGGTGCAGGATTGCTGTTGTTAATTCCGATAATGCTGAt                                                                                                                                                                                                                           <  2:70315/251‑1 (MQ=255)                ggTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGAtt                                                                                                                                                                                                                          <  1:159062/251‑1 (MQ=255)                         tCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATGCGGCAGGt                                                                                                                                                                                                                  <  1:209963/250‑1 (MQ=255)                                 gAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGGAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGa                                                                                                                                                                                                         <  2:83263/251‑1 (MQ=255)                                         ccGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTg                                                                                                                                                                                                 <  1:42188/251‑1 (MQ=255)                                               gtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAACTGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTAcc                                                                                                                                                                                           >  2:125810/1‑251 (MQ=255)                                               gtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCTGCAGGTGATTGTCGAACGTGCTGATTAc                                                                                                                                                                                            <  1:196770/250‑1 (MQ=255)                                               gtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTAcc                                                                                                                                                                                           <  1:12833/251‑1 (MQ=255)                                                    aCGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGc                                                                                                                                                                                      >  1:222379/1‑251 (MQ=255)                                                                 gCTGCGCAACGTGCAGGAAGGTGGAGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATCGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATg                                                                                                                                                                         >  2:139455/1‑251 (MQ=255)                                                                                               ggCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCaga                                                                                                                                           <  1:192567/251‑3 (MQ=255)                                                                                                      cTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGt                                                                                                                                    >  2:158153/1‑251 (MQ=255)                                                                                                              tCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGc                                                                                                                            >  1:160565/1‑251 (MQ=255)                                                                                                                aCCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTc                                                                                                                          >  2:35098/1‑251 (MQ=255)                                                                                                                                gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCAGTGCAAAGGAGAAGACTATGTTGAATTCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGTAAGATGCGTTTCGCCAAAGTTCCAGCGGGCCGCAAAAACTCGGTGGGCCGGTCATCGCTATTCCTGTGa                                                                                                          <  2:109950/251‑1 (MQ=255)                                                                                                                                                             aTCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGAt                                                                             <  2:48665/251‑1 (MQ=255)                                                                                                                                                                     gcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGt                                                                     <  2:69914/251‑1 (MQ=255)                                                                                                                                                                           gCAAAGGAGAAGTCTATGGTGAAATCCCCCCTGTTCTGGAAAATGACTTGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCGGATTACCGTAGCGATGTGGAAGATGCGAGTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAGCCGTGTAGCg                                                               <  2:85044/251‑1 (MQ=255)                                                                                                                                                                                  agaagaCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGGGCAGTATTGCTGTTGTTAATTCCTATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTTCCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGa                                                        <  2:30698/251‑1 (MQ=255)                                                                                                                                                                                                  aTCCCCCCTGTGCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCAttttt                                        <  2:178478/251‑1 (MQ=255)                                                                                                                                                                                                            ttCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTgg                              >  2:222806/1‑250 (MQ=255)                                                                                                                                                                                                                gaaaaaTGAATAGCCTGGTGGGTACAGTATTTCTGTGGTTAATTCCGATAATGCTGATTCGGCAGGTGAGTGTCGAACGTGCTGATTACCGTAGCGATGTGGAATATGCGATTCGACAAAGTACCAGCGGGCCTCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACTGTGCATGAAGAGGATAAAACCGTGGAGCGGCAACGAAGTTTTATCCATTTTTGGTTCCCTGAGTCa                          <  2:10955/249‑1 (MQ=255)                                                                                                                                                                                                                        aCTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCAttgatggt                  >  1:89772/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCAGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGTTGGATGGCa          <  1:171156/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATc       <  1:158153/250‑1 (MQ=255)                                                                                                                                                                                                                                        cAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAAc  >  2:4955/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           AAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAAC  >  NC_000913/4637993‑4638474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑