Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,800	G→T	L356F (TTG→TTT)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,800	0	G	T	100.0%	81.0 / NA	26	L356F (TTG→TTT)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/15);  total (11/15)

ATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGA  >  NC_000913/4631559‑4632038                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               aTCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGGTTTATTTCTGGAAcgc                                                                                                                                                                                                                                       >  1:15472/1‑251 (MQ=255)  tCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAGTTATTTCTgggaagcg                                                                                                                                                                                                                                      >  2:3501/1‑245 (MQ=255)   cAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAAcgcg                                                                                                                                                                                                                                      <  2:21348/250‑1 (MQ=255)        tCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGt                                                                                                                                                                                                                                 <  2:133137/250‑1 (MQ=255)            gAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGtgaagc                                                                                                                                                                                                                            <  1:94347/251‑1 (MQ=255)                 gcgcGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGtgaagctga                                                                                                                                                                                                                         <  1:236872/249‑1 (MQ=255)                        atCGTCGCCTGGCGGTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGGCGCCATTAGGCGCGCGCAATCTACGTCGCTTAGTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGGCGCGGCCGGAATTCCTGGCTGGCGCGTCTGCGGATGGAAGCGAGAGAGAAATATGAATGGCGTGACGGGCAGGCGGATGTATTTCTTGAACTCGGACGTGAAGCTGAAGCAAAaga                                                                                                                                                                                                                <  2:238770/251‑1 (MQ=255)                          cgtcgCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAagag                                                                                                                                                                                                               <  1:73855/250‑1 (MQ=255)                                              gCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCTGATTTATTTCTTGAACGCGGACGTGAAGCTGAATCAAAAGAGATTTTGCATCAACTCATGCaa                                                                                                                                                                                          <  2:235889/251‑1 (MQ=255)                                                  cGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGc                                                                                                                                                                                      <  2:90401/251‑1 (MQ=255)                                                           cgacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTTTGGAACGCGGACGTGAAGCTGAAACAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCt                                                                                                                                                                             >  1:193500/1‑251 (MQ=255)                                                           cgacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCt                                                                                                                                                                             >  1:33415/1‑251 (MQ=255)                                                            gacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTa                                                                                                                                                                            <  1:153504/251‑1 (MQ=255)                                                                                  aTGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGGTTTATTTCTGGAACGCCGACGTGAAGCTGCAGCAAAAGAGATTTTGCATTAACTCATGCAAACGCGTGGTTTCTACCCGATGGTTTCATCACCACGc                                                                                                                                                      >  2:35959/1‑251 (MQ=255)                                                                                     aCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATc                                                                                                                                                   <  1:197685/251‑1 (MQ=255)                                                                                      cGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCg                                                                                                                                                  <  1:49046/251‑1 (MQ=255)                                                                                       gCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGTACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCgg                                                                                                                                                 <  1:24851/251‑1 (MQ=255)                                                                                        ccATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGGTTTATTTCTGGAACGCGGGCGTGAAGCTGAAGCCAAAAAGATTTTTCATTAACTCATGCAACAGCGTGGTTTCTACCCGCTGGTTGCAGGACAACCCATCggg                                                                                                                                                >  2:120954/1‑250 (MQ=255)                                                                                             aTGCGCTCGAAATCTACTTCGCATATTGAACGCCGTGTACGAATGGCGCTTGGCCCCGGCGATCGTCGCGGACTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGGTTTATTTTTGCAACGCGGACGTGGAGGTGAAGGAAAAGAGATTTTGCCTCAACTTCTTCAAAAGCGTGGTTTCTACCCCATGGTTGCAGCCAAAAGCATCggggcagg                                                                                                                                           >  2:159464/1‑245 (MQ=255)                                                                                                                                     gAATGGCGCTCGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTTGAACGCGGGCGTGAAGCTGAAGCAAAAGAGATTTTGCCTCAACTCCTGCAACAGCGTGGTTTCTAACCGATGGGTGCAGCAAAACGCATCCGCGAAGAGGATGAGCTGAAGATTGATAAAGAGCCGCAGAATGTTGAc                                                                                                   >  1:194729/1‑251 (MQ=255)                                                                                                                                                          aTCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGGGAAGCTGAAGCAAAAGAGATTTTGCATCCACTCATGCAACAGCGGGGTTTTTACCCGGTGGGTGCAGCACCAAGCATCGGCGCAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCCgggggcg                                                                              >  2:229785/1‑248 (MQ=255)                                                                                                                                                                                                aTGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAtct                                        <  2:181184/251‑1 (MQ=255)                                                                                                                                                                                                         aaagagaaagaTGAATGGCGTTACTGGCAGGCGGAGTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTGCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATAc                               <  1:10110/251‑1 (MQ=255)                                                                                                                                                                                                             agaaagaTGAATGGCGTTACTGGCAGGCGGATTTTTTTCTAGAAAGCGGAAGTGAAGCTGAAGCAAAAAAGATTTTGCATCAACTCATGCAACAGCGTGGGTTCTACCCGATGGTTGCAGCACAAAGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCCGACCCCGGGGCCGGAGATGGCGCGCGTGCGCGCGGTGATGTCATGGGCTCTCGGTAATACcgcg                           >  1:24316/1‑251 (MQ=255)                                                                                                                                                                                                                            gTTACTGGCAGGCGGATTTATTTCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGcc            <  1:40150/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCGGATTTATTTCTGGCACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGa  >  1:85055/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               ATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGATTTATTGCTGGAACGCGGACGTGAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGA  >  NC_000913/4631559‑4632038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑