Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,489,016	C→T	E350K (GAA→AAA)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,489,016	0	C	T	100.0%	135.7 / NA	42	E350K (GAA→AAA)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/21);  total (21/21)

GGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGC  >  NC_000913/4488777‑4489254                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ggTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACCCCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCATTTTTGGTCTCAAACg                                                                                                                                                                                                                                     >  2:87522/1‑251 (MQ=255)    aCTGGCCTGAACGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCACTACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGGGACCAGTTCCTGAATCGCCTTTTTGGGATAAAACGCCg                                                                                                                                                                                                                                  >  2:11124/1‑251 (MQ=255)     cgggCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTAATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTTCTGGCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCgg                                                                                                                                                                                                                                 <  2:116339/249‑1 (MQ=255)      tGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCCTCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTTCCCAGTTCCTTCATCGCCTTTTTGGTATCAAACGCCgg                                                                                                                                                                                                                                 >  2:56418/1‑250 (MQ=255)                gCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCa                                                                                                                                                                                                                      <  2:83562/251‑1 (MQ=255)                  cTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATg                                                                                                                                                                                                                    >  1:126161/1‑251 (MQ=255)                      ttGCAACATCTCATAGGCGGATTCCCGGTCCACCTCCTTCTCATATTTTCCCTACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCCCCGGCCCCTTCCGCGAACACGGCGCGATCACCATCGCCCGCTCCACCA‑CAGAAGGGCTTCCTTTCGCCTCCAGCAAAGAGATCAAAGCCTCTCCGTAGCCCAGTTACTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCgtatctttt                                                                                                                                                                                                                >  2:190871/1‑243 (MQ=255)                       tGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTACCATCCACCGGAGAGTGATTAATCAAGCCATTCCGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCCTCCTTTCGCCTCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTg                                                                                                                                                                                                               >  2:26483/1‑251 (MQ=255)                                 cATAGGCGGACTCCAGATCAACCTCATCCTCCTATTTGCCATACACCGGACAGAGATTAATCACGCCATTACCCTCATCTTCCGCCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCCCCACCA‑CAGCAGGGCTTCCTTTCGCATCCAGAAAAGAGATCCACGCCTCGCCGGTGCCCAGTTCCTTAACCGCCTTTTTGGAATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGcc                                                                                                                                                                                                     >  2:27358/1‑251 (MQ=255)                                  aTAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCt                                                                                                                                                                                                    >  1:56379/1‑251 (MQ=255)                                   caGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACCCCGGAGAGTGAGAAATCAAGCCATTACGCTCATCTTCCGGCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTGCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGGTCCTGAATCGCCTTTTTGGTATCAAACGCGGGAGTGGTCCGCATGGTTTGCGCCGCTGCCtt                                                                                                                                                                                                   <  2:143132/250‑1 (MQ=255)                                                ggTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCAATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGCACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTg                                                                                                                                                                                       >  1:216643/1‑250 (MQ=255)                                                       ctcatcctcatATTTGCCATACACCGGAGAGTGATTAATCCAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTg                                                                                                                                                                               >  1:20625/1‑251 (MQ=255)                                                                           acacCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCAGCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTTCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGc                                                                                                                                                            <  1:115500/250‑1 (MQ=255)                                                                                       aaTTAAGCAAGCCATTACGCTCATCTTCCGTCCCCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGa                                                                                                                                               <  2:130711/250‑1 (MQ=255)                                                                                            aTCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTAcc                                                                                                                                          >  2:189814/1‑251 (MQ=255)                                                                                                   cATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGc                                                                                                                                   >  2:127105/1‑251 (MQ=255)                                                                                                                   cGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCg                                                                                                                   >  1:157468/1‑251 (MQ=255)                                                                                                                          ggCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGGGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGCTCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATc                                                                                                            >  2:172980/1‑251 (MQ=255)                                                                                                                                                            cACGCTC‑ACCAACAGAAGGCCTTACTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACg                                                                          >  1:208932/1‑251 (MQ=255)                                                                                                                                                                cTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCtt                                                                      >  2:85554/1‑251 (MQ=255)                                                                                                                                                                   cacca‑cAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACTGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTg                                                                   <  1:136120/251‑1 (MQ=255)                                                                                                                                                                     cca‑cAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAGCCAGACGCCCACGCCTTTTGAg                                                                 <  2:217456/251‑1 (MQ=255)                                                                                                                                                                      ca‑caGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGc                                                                <  1:79747/251‑1 (MQ=255)                                                                                                                                                                          aGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGCCGCCCACGCCTTTTGAGCGa                                                              >  2:198354/1‑250 (MQ=255)                                                                                                                                                                           gAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGGAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTgctcgaat                                                            <  2:92394/251‑8 (MQ=255)                                                                                                                                                                            aaGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATa                                                           <  2:124248/251‑1 (MQ=255)                                                                                                                                                                             aGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATaa                                                          <  1:159972/251‑1 (MQ=255)                                                                                                                                                                              gggCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAAGCGCCTTGTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTGTGGCGTAAAAGGCCGCAAAGCATGTGTAACGCGATTACCGAGCTGGCCGAGCACATTAGCCGGAAGATCAGACGTGTTTTGGGAAACGAGCCAGACGCCCACGCCTTTTGAGCGAATAAg                                                         <  1:106503/251‑1 (MQ=255)                                                                                                                                                                                   tCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCt                                                      <  2:41053/249‑1 (MQ=255)                                                                                                                                                                                    ccTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTAt                                                   >  2:13032/1‑251 (MQ=255)                                                                                                                                                                                          gCATCCAGAACAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCCCCCTTTTGGTATCAAACGCCTGATTGGCCCGCACTGTTTGCTCCGCTGCCTTCACTGCTTGCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCTGAATATCATACGGTTTTTGCGAAACGAACCAGACGCCGACGCTTTTTGATCGAATAAGCCTCATCACCt                                              <  2:24444/250‑1 (MQ=255)                                                                                                                                                                                              ccAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCa                                         <  1:64337/251‑1 (MQ=255)                                                                                                                                                                                                 gAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATc                                      >  1:172598/1‑251 (MQ=255)                                                                                                                                                                                                 gAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACAAGACGCCCACGCCGTTTGAGCGAATAAGCCTTATCACCTGCACAATc                                      <  2:39097/251‑1 (MQ=255)                                                                                                                                                                                                      gagaTCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATc                                 <  2:188926/251‑1 (MQ=255)                                                                                                                                                                                                         aTCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCcag                              <  1:85554/251‑1 (MQ=255)                                                                                                                                                                                                            aaCGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCTAGCACATTATCCGGAATATCATACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCcagcag                           <  1:132892/251‑1 (MQ=255)                                                                                                                                                                                                            aaCGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCcagcag                           <  2:56379/251‑1 (MQ=255)                                                                                                                                                                                                                                     gAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAcagc  >  2:185650/1‑251 (MQ=255)                                                                                                                                                                                                                                     gAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAca    <  1:61791/249‑1 (MQ=255)                                                                                                                                                                                                                                         cGCCTTTATGGTCTCACACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCATAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAAcagc  >  2:119028/1‑247 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GGTACTGGCCTGAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCA‑CAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGCATCGTTAAACAGC  >  NC_000913/4488777‑4489254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑