Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,605	G→A	S390N (AGC→AAC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,605	0	G	A	100.0%	32.1 / NA	11	S390N (AGC→AAC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

AGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGTT  >  NC_000913/4476387‑4476849                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     aGATAAGCTCTACGCATTAAATGATCACGGAATTGATCATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATa                                                                                                                                                                                                                       >  1:140494/1‑250 (MQ=255)      aGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAgcat                                                                                                                                                                                                                 <  1:100384/251‑1 (MQ=255)       gCTCTACGCATTAAATGATCACGGACTTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAgcatg                                                                                                                                                                                                                >  2:119425/1‑251 (MQ=255)            aCGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAgcatgca                                                                                                                                                                                                              <  2:212974/248‑1 (MQ=255)                                  tGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAAt                                                                                                                                                                                      <  1:129740/250‑1 (MQ=255)                                               gTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCa                                                                                                                                                                        <  2:103822/251‑1 (MQ=255)                                                 aGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAg                                                                                                                                                                       <  2:204082/250‑1 (MQ=255)                                                                                                                    gCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGATACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGAGTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCAGGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACAATATTTTTCAATCTCGATAAAGACCCCTATAAAAATAGCCCTGAATTACa                                                                                                   <  1:161474/251‑1 (MQ=255)                                                                                                                                                attcgattcTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCAGATTAATCTGCGCAGCGATCAGTCGAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAGCCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGta                                                                       <  1:104051/251‑1 (MQ=255)                                                                                                                                                                                          atgTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAAt                             >  1:136266/1‑251 (MQ=255)                                                                                                                                                                                                                       aaaaACATACAATATTTTGATGATACTAAAACGATAAAGCATGCATGTTCCGTAATAAATCATATTAATCTTCGAAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGTTAAAGAACCCTATAAAAATAGCCATGAATTACAGGGGTTGATTTGGAATAATTTGGTTGTATATGTCAATGAATTTATCTTATGTAATCGAGACAAAACAATTTTACTACAAAGGCTATTTGATAATGtt  >  2:173939/1‑249 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     AGATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGGTTGTATATGTCAATGAATTTAACTTAAGTAATCGAGAAAAAACAAATTTAATACAAAGGCTATTTGATAATGTT  >  NC_000913/4476387‑4476849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑