Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,217,310	A→G	S68G (AGC→GGC)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,217,310	0	A	G	100.0%	54.7 / NA	17	S68G (AGC→GGC)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/11);  total (6/11)

AAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGT  >  NC_000913/4217078‑4217551                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  aaaCCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACATTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAGCTGGGCGCAGCTAAAATGTGGGGTCTGCTGCGCTGTGGGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCactgact                                                                                                                                                                                                                                  <  1:21205/250‑1 (MQ=255)        cacaTAACTATGGAGCATCTGCACATGAAAACCCGTACCCAACAAATTGAAGAATTACCGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCGCGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGta                                                                                                                                                                                                                           >  1:26721/1‑249 (MQ=255)            tAACTATGGAGCATCTGCACATGAAAACCCGTACACAAAAAATTGAAGAATTACAGAAAGAGCGGACTCAACCGCGTTGGGAAGGCATTACTCGCACATACAGTGCGGAAGATGTGGTGAAATTACGCGGTGCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGgcg                                                                                                                                                                                                                      >  1:161339/1‑251 (MQ=255)                                                 acaaATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTTGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACGGGGCGCAGCGAAAATGTGGCGGCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGTCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATc                                                                                                                                                                                 <  1:38057/251‑1 (MQ=255)                                                        gaagaaTTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCggg                                                                                                                                                                          <  1:113195/251‑1 (MQ=255)                                                         aagaaTTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGa                                                                                                                                                                         <  1:164283/251‑1 (MQ=255)                                                              ttACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGc                                                                                                                                                                     >  1:208618/1‑250 (MQ=255)                                                                                   ccGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCt                                                                                                                                               <  2:53150/251‑1 (MQ=255)                                                                                             aaGGCATTACTCGCCCATCCAGTGCGGACGATGTGGTGCAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTAAGGGGCTCCAACAGGCGCAAGCGGGTACTGAAGCAGTCTATCTGTCGGGATGGCAGGGAGCGGCGGGCGCTAACCcggcgggccgc                                                                                                                                     >  2:24311/1‑246 (MQ=255)                                                                                                             aTACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGGATCCGGATCAGt                                                                                                                     >  1:237879/1‑251 (MQ=255)                                                                                                                                        aCGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAg                                                                                          <  1:183613/251‑1 (MQ=255)                                                                                                                                         cgcgGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGc                                                                                         <  1:57972/251‑1 (MQ=255)                                                                                                                                                      aTCCTGCATGCAAGCTGGCGCCACTGCGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAAAGGCCTAGGCGCACTGACTGGCGGACAGGCGCTGCAACAGGCGAAAGCGGGTCTTGAAGCAGACTATCTGTCGGGATGGCCGGGAGCGGCGGGCGCTAACCTGGCGGCCAGCATGGATACGGGTCAGCCGCTCTATCCGGCCAACTCGGTGCCaacctggggggggcgg                                                                            >  2:105528/1‑236 (MQ=255)                                                                                                                                                               gCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATcaacaa                                                                    <  1:141000/250‑1 (MQ=255)                                                                                                                                                                               gggCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCg                                                   <  2:208618/251‑1 (MQ=255)                                                                                                                                                                                                                        aaaaaGGCTACATCAACGGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCcg          <  1:30250/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCATCAACGGCCTCGGCGCACTGACTGGCGGTCAGACGCTGCAACCGGCGAAAGCTGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATTTATCCGGATCAGTCGCTCTATCCGGCAAACTCTGTGCCAGCTGTGGTGGAGCGGATCAACACCACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGt  <  1:41260/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  AAACCACCACATAACTATGGAGCATCTGCACATGAAAACCCGTACACAACAAATTGAAGAATTACAGAAAGAGTGGACTCAACCGCGTTGGGAAGGCATTACTCGCCCATACAGTGCGGAAGATGTGGTGAAATTACGCGGTTCAGTCAATCCTGAATGCACGCTGGCGCAACTGGGCGCAGCGAAAATGTGGCGTCTGCTGCACGGTGAGTCGAAAAAAGGCTACATCAACAGCCTCGGCGCACTGACTGGCGGTCAGGCGCTGCAACAGGCGAAAGCGGGTATTGAAGCAGTCTATCTGTCGGGATGGCAGGTAGCGGCGGACGCTAACCTGGCGGCCAGCATGTATCCGGATCAGTCGCTCTATCCGGCAAACTCGGTGCCAGCTGTGGTGGAGCGGATCAACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGT  >  NC_000913/4217078‑4217551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑