Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,996	T→G	D70A (GAT→GCT)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,996	0	T	G	100.0%	65.1 / NA	23	D70A (GAT→GCT)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/13);  total (10/13)

GTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCC  >  NC_000913/4193763‑4194239                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    gTTCCAGCCCCTGCGGTGGAGAATGCATCTGTTTGGTTTGCGTCGGGAACACATGCCCCAGCGCGATATAAGTGGGGCGTGCTGCCAGCGTGACGTCGATTTCCATATCGTGGTGTGTGGAAACGCCCAGCCCCTGGCCTGCCTTGCGGATGTCATTGAGGTCGGTGGGTTGCAAATCTTCCTGCCCGAATTTGAGGCGATACGCCTGATCGTTGTGCGCCAGCCGCCAGTAAGCGTTGATAAACAATcg                                                                                                                                                                                                                                     <  1:215255/250‑1 (MQ=255)                           gtcggttgggttggcgcggggAACCCATGTCCCAGCGCGATATAAGAGGGGCGGGCCGCCATCGCGACGTCGATTTCCAGATCGTCATGTGTCAAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAGATGGACGCCATTCGCCTGATGCTTGATCGCCATCCGCCAGTAAGCGTTGATAAACAATCGCTCGTGATAGCGACGGCCCTTCGCAAt                                                                                                                                                                                                          <  2:95310/233‑1 (MQ=255)                                    gttggggtggggaacaaagtacccggcgcAATAAAAGGGGGGGGTGCTGCCGGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACCCCCAGCCGCAGCCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTGGCAAATCTTCCTGCCCCAAATGGCCGCCATCCGCCTGAGGCTTAATCTCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCAc                                                                                                                                                                                                 <  2:136271/226‑1 (MQ=255)                                      ttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATAGCGCGTTATAGCGGCGGCCCAGCGCAAATgccgcccagc                                                                                                                                                                                               >  2:157261/1‑247 (MQ=255)                                                                                     caagcgcgactgtgATTTCAATATCGTCATGTGTCGAACCGCCCAGCCGCAGGCCTGCCGCTCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTgg                                                                                                                                                <  2:87079/238‑1 (MQ=255)                                                                                        gcgcGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTgg                                                                                                                                                >  1:35644/1‑248 (MQ=255)                                                                                          gcgACGACGGTTTCCATATCGTCATGTGTCGAACCGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTACGCGTTGATAAACAATCGCGCGTTATACCGGCGGCCCAGCGCCATTTCCGCCCCGACATCGGCTTTCCCCTCTTCATCGCGCCGATATTTGATGCGGAGCTGGagagg                                                                                                                                           >  2:156951/1‑250 (MQ=255)                                                                                                 ggtgtcccaattcgtcttgggccaaaaCGCCCGGCTGCAGACCTGCCGCGCGGAGGGAATTGAGAGCGGGGGCTTGCAAATTTTCCTGCCCCAAATGGACGCCATACGCCGGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGAAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGtacgtacg                                                                                                                                    <  2:3794/227‑1 (MQ=255)                                                                                                                        gAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTCGCTGGAGAGTACGTACGCCTGCATCCAACATAcgatcgat                                                                                                             >  2:60851/1‑251 (MQ=255)                                                                                                                                                   cgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAAAAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGt                                                                                   >  2:222267/1‑250 (MQ=255)                                                                                                                                                       gggCATTGCGATCGGGGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGTCCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGt                                                                              <  2:53223/250‑1 (MQ=255)                                                                                                                                                                   cGGTGGCTTGCTAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGAACTTTGATGCGTAGCTGGAGAGTCCGTACGCCTGCATCCAACAGACGTTCGCTCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaaa                                                                   >  2:77691/1‑250 (MQ=255)                                                                                                                                                                    ggTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGaaa                                                                   >  1:215211/1‑249 (MQ=255)                                                                                                                                                                              aaaTCTCCCTGCCCCAAAGGGCCGCCATACGCCTGATGATGGAGCGCCGGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACaggagg                                                       <  2:72505/251‑1 (MQ=255)                                                                                                                                                                                          cccAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTg                                           <  1:92785/251‑1 (MQ=255)                                                                                                                                                                                            caattgtaCGCCATACTCCTGATTCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGGACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGAt                                         <  1:142768/244‑1 (MQ=255)                                                                                                                                                                                                         cccGCCTGATGCTTGTCCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGCCCCAGCACAATTGCCGCCCCGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcc                            <  2:41098/249‑1 (MQ=255)                                                                                                                                                                                                                gatcctttatcgccgcccgccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAAGTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTa                      <  2:57458/234‑1 (MQ=255)                                                                                                                                                                                                                gATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGAGCGTTATCGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTAc                     >  1:121402/1‑251 (MQ=255)                                                                                                                                                                                                                  tGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGAAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACg                    <  2:163497/250‑1 (MQ=255)                                                                                                                                                                                                                              aGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtct       >  2:184617/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccAGTAAGCGTTGATAACCAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGcc  <  2:203657/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGcc  >  1:78225/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    GTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCC  >  NC_000913/4193763‑4194239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑