Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,502	G→T	P226Q (CCG→CAG)	rarD ←	putative transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,502	0	G	T	100.0%	25.8 / NA	10	P226Q (CCG→CAG)	rarD	putative transporter RarD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/6);  total (4/6)

TCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACA  >  NC_000913/4003257‑4003748                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         tCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGctgtacg                                                                                                                                                                                                                                                     >  1:107003/1‑251 (MQ=255)                                      ggACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGCAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGAGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACCGCTGTACGGTAGGTAATAAAACCG‑CGGCGTTCAGCAGTAAATTc                                                                                                                                                                                                                >  1:234361/1‑250 (MQ=255)                                                                                     gcgcCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATa                                                                                                                                                               <  2:45740/251‑1 (MQ=255)                                                                                             cAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGGAAAACACAACATATGTAAGGTAG‑TGACAATACCGGGGGGGATCAACAGTAAATTCAGCGAAATCGGGGTTTTCCCCCA‑CTGGCTTGTTGAGATGTCGGGAATAGccaaacg                                                                                                                                                       >  1:199279/1‑245 (MQ=255)                                                                                                   aaGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAG‑TGACCATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑GTTTGCCCCATATGGCTGGTTGAGCCGTCGGCAATAGCAAACAGGTAAAt                                                                                                                                                 >  1:227499/1‑251 (MQ=255)                                                                                                            gTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAG‑GGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCAc                                                                                                                                        <  2:227499/251‑1 (MQ=255)                                                                                                                     ttATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAAAACAACAGCTGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAACTGTCGGCAATAGCAAAAAGGTAAATTGCCGCCACCGgcagccg                                                                                                                               >  1:73166/1‑249 (MQ=255)                                                                                                                                               aaaCGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCgg                                                                                                     <  1:228924/251‑1 (MQ=255)                                                                                                                                                       cagccagcAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCt                                                                                             <  2:173415/251‑1 (MQ=255)                                                                                                                                                                                gCCAAGGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCTGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCa                                                                    <  2:171234/251‑1 (MQ=255)                                                                                                                                                                                                                                                  caGCTGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCcaca  <  1:262/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         TCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAG‑TGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGG‑TTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATAGCAAACAGGTAAATTGCCGCCACGGGCAGCAGCCACATGGTTTCGATTAACATGCCGGTTTGCGCTTCAACGGCAATCTTCTTGCGTACCAGACCGTAGAAGGCAAAACTAAATGCCAGTCCCAGCGCGATAATAGGTAGCGAACCAAAAGTCCACA  >  NC_000913/4003257‑4003748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑