Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,834	C→T	intergenic (‑201/+178)	mnmG ← / ← mioC	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG/flavoprotein MioC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,834	0	C	T	100.0%	81.4 / NA	25	intergenic (‑201/+178)	mnmG/mioC	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG/flavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

GACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCA  >  NC_000913/3925588‑3926078                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTAGGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAAcc                                                                                                                                                                                                                                                  <  2:123728/251‑1 (MQ=255)            atgatgatgACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATc                                                                                                                                                                                                                                       <  1:71605/251‑1 (MQ=255)             tgatgatgaCGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATc                                                                                                                                                                                                                                       >  1:62374/1‑250 (MQ=255)                                        aaaaCATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTg                                                                                                                                                                                                            <  2:967/250‑1 (MQ=255)                                                  gATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGtata                                                                                                                                                                                                   >  1:47289/1‑249 (MQ=255)                                                     tGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTg                                                                                                                                                                                              >  1:60478/1‑251 (MQ=255)                                                                            aaTGGATTGAAGCCCGGGCCGAGGATTCTACTCACCTTTGTCGGCTTGAGCAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGAACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGTTCAGAATGAATGGTTATac                                                                                                                                                                        >  2:230461/1‑250 (MQ=255)                                                                                   tGAAGTCCGGGCCGTGGATTCTACTCAACTTTGTCGGCGTGAGAAAGACCGGGGATCCTGGGTATTAAAAAGCAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGAACGCACTGCCCTGTGGATAACAAGAATCCAGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCGGGGAGCAGAATGAGCGGTTATCcacagcgc                                                                                                                                                                 <  2:198744/250‑5 (MQ=255)                                                                                                      tCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAgttg                                                                                                                                              <  2:46410/250‑1 (MQ=255)                                                                                                         aCTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCt                                                                                                                                          >  1:204357/1‑251 (MQ=255)                                                                                                            cAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTAGTAGGATCGCACTGCCCTGTGGATAACAAGGATCCTGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGTTGATCCTGGACCGTATAAGCTGGGATCTGAAGGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTg                                                                                                                                       <  1:37878/251‑1 (MQ=255)                                                                                                                tttGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGAt                                                                                                                                    >  1:168370/1‑250 (MQ=255)                                                                                                                       gCTTGAGAAAGACTTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGCGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCg                                                                                                                            >  2:98452/1‑251 (MQ=255)                                                                                                                                                  aTTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGAGATCTCTTATTAGGATCGCACTGCCCTGTGGATAGCAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGtt                                                                                                  >  2:113809/1‑250 (MQ=255)                                                                                                                                                         agaagaTCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTGTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGAGCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTGGATCCAAGCTTCCTGACAGAGTTTGCCACAg                                                                                          <  2:157239/251‑1 (MQ=255)                                                                                                                                                          gaagaTCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGt                                                                                         >  1:94671/1‑251 (MQ=255)                                                                                                                                                                  atttatttAGAGATCTGTTCTATTGTGATCTCTTAGTAGTATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTGTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAAGGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGTGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGAt                                                                                     <  2:199357/247‑1 (MQ=255)                                                                                                                                                                          agagATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCTGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTTGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTATATCGCACGATCTGt                                                                         <  2:148465/251‑1 (MQ=255)                                                                                                                                                                          agagATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGt                                                                         >  1:99588/1‑251 (MQ=255)                                                                                                                                                                           gagaTCTGTTCTATTGTGCTCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTCGATCGCACGATCTGt                                                                         >  2:102215/1‑250 (MQ=255)                                                                                                                                                                                        ttGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATAGCACGATCTGTATACTTATTTGAGt                                                           >  1:128051/1‑251 (MQ=255)                                                                                                                                                                                                 ctTATTAGGATCGCACTCACCAGTGGATAACAACGATCCGGCTTTTAAGATCACTAAAATGGAAAGGACAATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGGTCTACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGATTCCTGACAGAGTTATCCACAGAAGATCGCACGATCTGTATACATATTTGCGTAAATTAAcc                                                  >  2:111237/1‑251 (MQ=255)                                                                                                                                                                                                             gCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTGACCCACGATCCCAGc                                      <  2:233403/251‑1 (MQ=255)                                                                                                                                                                                                                                aaGGATCCGGCTTTTAAGATCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACATTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCtt                   <  1:237215/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aTCAATAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCa  >  1:121969/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GACCCCCGCCAATGATGATGACGTCAAAAGGATCCGGATAAAACATGGTGATTGCCTCGCATAACGCGGTATGAAAATGGATTGAAGCCCGGGCCGTGGATTCTACTCAACTTTGTCGGCTTGAGAAAGACCTGGGATCCTGGGTATTAAAAAGAAGATCTATTTATTTAGAGATCTGTTCTATTGTGATCTCTTATTAGGATCGCACTGCCCTGTGGATAACAAGGATCCGGCTTTTAAGATCAACAACCTGGAAAGGATCATTAACTGTGAATGATCGGTGATCCTGGACCGTATAAGCTGGGATCAGAATGAGGGGTTATACACAACTCAAAAACTGAACAACAGTTGTTCTTTGGATAACTACCGGTTGATCCAAGCTTCCTGACAGAGTTATCCACAGTAGATCGCACGATCTGTATACTTATTTGAGTAAATTAACCCACGATCCCAGCCATTCTTCTGCCGGATCTTCCGGAATGTCGTGATCA  >  NC_000913/3925588‑3926078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑