Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,770,539	C→T	V281M (GTG→ATG)	yibH ←	inner membrane protein YibH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,770,539	0	C	T	100.0%	82.7 / NA	23	V281M (GTG→ATG)	yibH	inner membrane protein YibH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

GAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGC  >  NC_000913/3770307‑3770777                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               gAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGcc                                                                                                                                                                                                                              >  1:52775/1‑251 (MQ=255)             aaaTGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACg                                                                                                                                                                                                                  <  2:225993/251‑1 (MQ=255)                 ggTCGGAGTAAACCGCCCCCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAAc                                                                                                                                                                                                              <  1:88708/251‑1 (MQ=255)                     ggAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCg                                                                                                                                                                                                          >  2:78652/1‑251 (MQ=255)                                                       ggTAAGGAATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGCCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGttt                                                                                                                                                                        >  2:216553/1‑251 (MQ=255)                                                                 cGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCggg                                                                                                                                                               <  2:147330/250‑1 (MQ=255)                                                                                ggTCCAGTTCCATGGTTCCCAGCACACCCTCCGTGACGGGCACGACCGTTAATGATTGCCATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGCGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCAcc                                                                                                                                               >  2:15945/1‑251 (MQ=255)                                                                                  tCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAAGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCgg                                                                                                                                             >  1:30730/1‑251 (MQ=255)                                                                                             ggTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGc                                                                                                                                   >  2:220774/1‑250 (MQ=255)                                                                                                                   ccGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTgg                                                                                                            <  2:155690/251‑1 (MQ=255)                                                                                                                     gggCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGc                                                                                                          >  1:170155/1‑251 (MQ=255)                                                                                                                                  aaTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATg                                                                                                                   >  1:51669/1‑229 (MQ=255)                                                                                                                                  aaTGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATg                                                                                                                   <  2:51669/229‑1 (MQ=255)                                                                                                                                         gCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACg                                                                                      <  1:45303/251‑1 (MQ=255)                                                                                                                                          cAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGt                                                                                     >  2:7917/1‑251 (MQ=255)                                                                                                                                                  ccTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTg                                                                             >  1:191811/1‑251 (MQ=255)                                                                                                                                                     gcgcCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGc                                                                            <  2:41326/249‑1 (MQ=255)                                                                                                                                                     gcgcCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCt                                                                           <  1:181851/250‑1 (MQ=255)                                                                                                                                                                  aCCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATg                                                              >  1:51423/1‑250 (MQ=255)                                                                                                                                                                                                               cacCTGCCCAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTa                <  2:191679/251‑1 (MQ=255)                                                                                                                                                                                                                      ccAGGTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTcac         <  1:127971/251‑1 (MQ=255)                                                                                                                                                                                                                         ggTAGCGCGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAAt      >  2:51559/1‑251 (MQ=255)                                                                                                                                                                                                                             gcgcGTTAAACATCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGc  <  2:5230/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                               GAAACATGGCTGAAATGGTCGGAGTAAACCGCCACCTGGGCGTAGATGCCGTCGGGTAAGGCATCGATATCATCGTTAGGGTCCAGTTCAATGGTTCCCAGCACACCGTCCGTGCCGGGCACGACCGTTAATGATTGCAATACCCCCTGCGCCTGATAAGAACCGCCTGGCACGACAGGTAAAATACTAGTCAGTTTGCCGTGAAACACCTGCCCAGGTAGCGCGTTAAACACCACTTCCGCATCATCGCCAGGTTTCAGACGTAACAGCGAGTTTTGCCGAAATTGGGCGACAATTTGCCGTTTTTGCTCGGGGATGAAGACCATCACCGGACGCAGCGGCAAGGCAGCTGCGTATGTACCTGGGCGGATCAGTACCTGAGTAACGTAGCCATTGCTCGGCGCGCGAATGACAGTCTGCTCAAGGTTATATTTTGCTTCAGTAAGTTGCGCTCTTAAGCTCACAATCTGC  >  NC_000913/3770307‑3770777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑